Device and method for processing target component in tube

Patent metadata

Field	Value
Publication number	US-9517472-B2
Application number	US-201113995270-A
Country	US
Kind code	B2
Filing date	Jul 13, 2011
Priority date	Dec 21, 2010
Publication date	Dec 13, 2016
Grant date	Dec 13, 2016

Abstract

The present invention provides a small and low running-cost device capable of minimizing the generation of contamination sources as much as possible while performing a series of all the desired manipulations. A device for manipulating a target component in a manipulation tube, comprising: a manipulation tube comprising a tube having an optionally-closeable open end for supplying a sample containing a target component at one end and a closed end at the other end, and a manipulation medium accommodated in the tube and having a gel layer and an aqueous liquid layer multilayered in a longitudinal direction of the tube; magnetic particles that should transport the target component; and magnetic field applying means capable of applying a magnetic field to the manipulation tube to move the magnetic particles in the longitudinal direction of the tube.

First claim

The invention claimed is:

1. A device for manipulating a target component in a manipulation tube, comprising:
a manipulation tube comprising a tube having an optionally-closeable open end for supplying a sample containing a target component at one end and a closed end at the other end, and a manipulation medium accommodated in the tube and having a gel layer and an aqueous liquid layer alternately layered in a longitudinal direction of the tube, wherein the gel layer is a gel plug provided on either side of the aqueous liquid layer so that the aqueous liquid layer is sandwiched between the gel plugs in the longitudinal direction of the tube, and is fixed in a predetermined position in the tube;
magnetic particles that should capture and transport the target component; and
a magnetic field applying member capable of applying a magnetic field to the manipulation tube to move the magnetic particles in the longitudinal direction of the manipulation tube through the gel layer in a gel state.

2. The device according to claim 1 , wherein the tube has an approximate inner diameter of 0.1 mm to 5 mm.

3. The device according to claim 1 , wherein the magnetic field applying member can move the magnetic particles in the longitudinal direction of the manipulation tube from an outside position of the manipulation tube.

4. The device according to claim 1 , further comprising a holding member capable of holding the manipulation tube almost vertically in such a manner that the open end faces upward.

5. The device according to claim 4 , wherein the holding member is a holding substrate having a plurality of holding holes capable of holding the closed end of the manipulation tube.

6. The device according to claim 4 , wherein the holding member has a temperature control function.

7. The device according to claim 4 , wherein the holding member has a recess allowing the magnetic field applying member to be moved in the longitudinal direction of the manipulation tube.

8. The device according to claim 4 , wherein the holding member has an optical detection port.

9. The device according to claim 1 , wherein the magnetic field applying member has a system that controls movement of a magnetic field in the longitudinal direction of the manipulation tube and strength of the magnetic field.

10. The device according to claim 1 , wherein the magnetic field applying member has a temperature control function.

11. The device according to claim 9 , wherein the magnetic field applying member comprises a movable substrate capable of moving in the longitudinal direction of the manipulation tube, and a plurality of magnetic sources held in the movable substrate.

12. The device according to claim 11 , wherein the system that controls movement of a magnetic field in the longitudinal direction of the manipulation tube and strength of the magnetic field simultaneously moves the plurality of magnetic sources in the movable substrate.

13. The device according to claim 1 , wherein the magnetic particles have an ability to bind to or adsorb to nucleic acid as the target component, and the manipulation medium includes an aqueous liquid layer composed of a liquid that liberates the nucleic acid to bind or adsorb the nucleic acid to the magnetic particles and/or an aqueous liquid layer composed of a liquid for washing the magnetic particles.

14. The device according to claim 13 , wherein the manipulation medium further includes an aqueous liquid layer composed of a nucleic acid amplification reaction liquid; or an aqueous liquid layer composed of a reverse transcription reaction liquid and an aqueous liquid layer composed of a nucleic acid amplification reaction liquid.

15. The device according to claim 1 , wherein the tube is integrally formed.

16. The device according to claim 1 , wherein
the manipulation tube has a manipulation part A and a recovery part B;
the tube constituting the manipulation tube has a manipulation tube portion a and a recovery tube portion b corresponding to the manipulation part A and the recovery part B, respectively;
the manipulation part A comprises the tube portion a, and the manipulation medium accommodated in the tube portion a; and
the recovery part B comprises the tube portion b, and a recovery medium accommodated in the tube portion b and including at least one of an aqueous liquid layer and a gel layer.

17. The device according to claim 16 , wherein the manipulation tube portion a and the recovery tube portion b are separable from each other.

18. The device according to claim 1 , wherein the tube is made of a material selected from the group consisting of polyethylene, polypropylene, fluorine resin, polyvinyl chloride, polystyrene, polycarbonate, acrylonitrile butadiene styrene copolymer, acrylonitrile styrene copolymer, acrylic resin, polyvinyl acetate, polyethylene terephthalate, cyclic polyolefin, and glass.

19. A manipulation tube for manipulating a target component, comprising:
a tube having an optionally-closeable open end for supplying it sample containing a target component at one end and a closed end at the other end;
a manipulation medium accommodated in the tube and having a gel layer and an aqueous liquid layer alternately layered in a longitudinal direction of the tube, wherein the gel layer is a gel plug provided on either side of the aqueous liquid layer so that the aqueous liquid layer is sandwiched between the gel plugs in the longitudinal direction of the tube, and is fixed in a predetermined position in the tube; and
magnetic, particles that should capture and transport the target component,
wherein the manipulation tube is constituted so that the magnetic particles are moved in the longitudinal direction of the manipulation tube through the gel layer in a gel state by applying a magnetic field to the manipulation tube.

Assignees

• Ohashi Tetsuo
• Shimadzu Corp

Inventors

• Ohashi Tetsuo

Classifications

• B03C1/30

  Combinations with other devices, not otherwise provided for · CPC title

• B03C2201/18

  Magnetic separation whereby the particles are suspended in a liquid · CPC title

• B03C1/01

  by addition of magnetic adjuvants · CPC title

• B03C1/0332

  using permanent magnets · CPC title

• B03C1/288

  disposed at the outer circumference of a recipient · CPC title