Bioactive protein-polymer compositions for stain removal

Patent metadata

Field	Value
Publication number	US-12134751-B2
Application number	US-202318327433-A
Country	US
Kind code	B2
Filing date	Jun 1, 2023
Priority date	Jun 21, 2010
Publication date	Nov 5, 2024
Grant date	Nov 5, 2024

Abstract

Bioactive coatings that include a base and a protein associated with the base for actively promoting the removal of organic stains are provided. In aspects, bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.

First claim

What is claimed is:

1. A method of facilitating removal of an organic stain from a substrate or coating comprising:
providing a substrate or coating formed of an organic crosslinkable polymer resin;
associating a lipase with said substrate or said coating, said lipase capable of enzymatically degrading a component of an organic stain, wherein said lipase is uniformly dispersed in said substrate or said coating in protein aggregates having an average diameter of 10 micrometers or less; and
facilitating removal of an organic stain from said substrate or said coating by rinsing.

2. The method of claim 1 , wherein said lipase-associated substrate or said coating has a surface activity of about 200 Units/gram.

3. The method of claim 1 , wherein said lipase is present at 0.1-50 weight percent of the total weight of said substrate or said coating.

4. The method of claim 1 , wherein said lipase is present at 1-20 weight percent of the total weight of said substrate or said coating.

5. The method of claim 1 , wherein said lipase is present at 2-8 weight percent of the total weight of said substrate or said coating.

6. The method of claim 1 , wherein said lipase is associated with said substrate or said coating by a method comprising mixing an aqueous solution comprising said lipase with an admixture, wherein said aqueous solution is substantially free of surfactant, to produce an emulsion.

7. The method of claim 6 , wherein said admixture comprises a polymer resin, a surfactant, and a non-aqueous organic solvent.

8. The method of claim 1 , wherein said organic stain is a bacterial organic stain.

9. The method of claim 8 , wherein said lipase is entrapped within and crosslinked to said substrate or said coating.

10. The method of claim 8 , wherein said organic crosslinkable polymer resin comprises a functional group of acetoacetate, acid, amine, carboxyl, epoxy, hydroxyl, isocyanate, silane, or vinyl, or a combination thereof.

11. The method of claim 8 , wherein said organic crosslinkable polymer is a hydroxyl-functionalized acrylate resin.

12. The method of claim 1 , wherein said organic crosslinkable polymer resin is an aminoplast, melamine formaldehyde, carbamate, polyurethane, polyacrylate, epoxy, polycarbonate, alkyd, vinyl, polyamide, polyolefin, phenolic resin, polyester, or polysiloxane, or a combination thereof.

Assignees

• Toyota Motor Corp
• Univ Minnesota

Inventors

• Buthe Andreas
• Wang Ping
• Wu Songtao
• Jia Hongfei
• Ishii Masahiko
• Zhang Minjuan

Classifications

• C11D2111/18

  Glass; Plastics · CPC title

• D06M16/003

  with enzymes or microorganisms · CPC title

• C12Y304/24027

  Thermolysin (3.4.24.27) · CPC title

• C11D3/43

  Solvents · CPC title

• C11D3/201

  linear · CPC title