Thermolysin-like protease for cleaning insect body stains

Patent metadata

Field	Value
Publication number	US-9388370-B2
Application number	US-201213567341-A
Country	US
Kind code	B2
Filing date	Aug 6, 2012
Priority date	Jun 21, 2010
Publication date	Jul 12, 2016
Grant date	Jul 12, 2016

Abstract

A substrate or coating is provided that includes a protease with enzymatic activity toward a component of a biological stain. Also provided is a process for facilitating the removal of a biological stain is provided wherein an inventive substrate or coating including a protease is capable of enzymatically degrading of one or more components of the biological stain to facilitate biological stain removal from the substrate or said coating.

First claim

The invention claimed is:

1. A method of facilitating the removal of a biological stain containing a protein component on a substrate or a coating comprising:
providing a liquid coating material;
associating an active extracellular portion of bacterial neutral thermolysin-like protease from Geobacillus stearothermophilus with said liquid coating material to form a liquid bioactive coating material at a pH of 10.0 to 11.0, such that said protease is capable of enzymatically degrading a protein component of a biological stain, wherein said protease has a specific activity in excess of 20,000 U/g in said liquid bioactive coating material.

2. The method of claim 1 wherein said protease is in solution in said liquid bioactive coating material.

3. The method of claim 1 wherein said liquid coating material comprises an alcohol, said alcohol present at less than 0.8% by weight.

4. The method of claim 1 wherein said liquid coating material comprises ammonia, or a derivative of ammonia.

5. The method of claim 1 further comprising applying said liquid bioactive coating material to a biological stain on a coating material or substrate, thereby promoting removal of said stain.

6. The method of claim 5 wherein said biological stain is an insect stain.

7. The method of claim 5 wherein said biological stain is present on a substrate.

8. The method of claim 7 wherein said substrate is glass.

9. A liquid bioactive coating material for facilitating removal of a biological stain containing a protein component comprising:
a liquid coating material; and
an active extracellular portion of bacterial neutral thermolysin-like protease from Geobacillus stearothermophilus , said protease capable of degrading a biological stain containing a protein component wherein said protease is associated with said liquid coating material,
wherein the liquid bioactive coating material has a pH of 10.0 to 11.0, wherein said protease has a specific activity in excess of 20,000 U/g in said liquid bioactive coating material.

10. The liquid bioactive coating material of claim 9 wherein said protease is in solution.

11. The liquid bioactive coating material of claim 9 wherein said liquid bioactive coating material comprises an alcohol, said alcohol present at less than 0.8% by weight.

12. The liquid bioactive coating material of claim 9 wherein said liquid bioactive coating material comprises ammonia, or a derivative of ammonia.

Assignees

• Wu Songtao
• Jia Hongfei
• Ishii Masahiko
• Zhang Minjuan
• Toyota Eng & Mfg North America
• Toyota Motor Corp

Inventors

• Wu Songtao
• Jia Hongfei
• Ishii Masahiko
• Zhang Minjuan

Classifications

• C11D3/2003

  Alcohols; Phenols · CPC title

• C11D3/37

  Polymers {(polymeric surface-active agents C11D1/008; polysaccharides C11D3/222)} · CPC title

• C11D7/261

  Alcohols; Phenols · CPC title

• C11D7/04

  Water-soluble compounds · CPC title

• C11D3/38618

  Protease or amylase in liquid compositions only · CPC title