Thermolysin for easy-cleaning of insect body stains

Patent metadata

Field	Value
Publication number	US-10767141-B2
Application number	US-201615193242-A
Country	US
Kind code	B2
Filing date	Jun 27, 2016
Priority date	Jun 21, 2010
Publication date	Sep 8, 2020
Grant date	Sep 8, 2020

Abstract

A substrate or coating is provided that includes a protease with enzymatic activity toward a component of a biological stain. Also provided is a process for facilitating the removal of a biological stain is provided wherein an inventive substrate or coating including a protease is capable of enzymatically degrading of one or more components of the biological stain to facilitate biological stain removal from the substrate or said coating.

First claim

The invention claimed is:

1. A method of facilitating the removal of a biological stain on a substrate or a coating comprising:
providing a liquid coating material comprising an alcohol selected from the group consisting methanol, ethanol, isopropyl alcohol, propanol and butanol, wherein the concentration of said alcohol in said liquid coating is from 20 to 40% wt. %, wherein the pH of the liquid coating is geater than 5.0, and associating a themolysin-like protease, wherein the protease has an activity in excess of 20,000 U/mg, with said liquid coating material to form a liquid bioactive coating material such that said protease is capable of enzymatically degrading a component of a biological stain.

2. The method of claim 1 wherein the pH of the liquid bioactive coating material is greater than 8.0.

3. The method of claim 1 wherein said protease is in solution in said liquid bioactive coating material.

4. The method of claim 1 further comprising applying said bioactive coating material to a biological stain comprising a protein component, thereby promoting removal of said stain.

5. The method of claim 4 wherein said biological stain is an insect stain.

6. The method of claim 4 wherein said biological stain is present on a substrate.

7. The method of claim 6 wherein said substrate is glass.

8. The method of claim 1 wherein said alcohol is methanol, and said stain is an insect stain.

9. The method of claim 1 wherein, said thermolysin-like protease is an extracellular fragment of a bacterial neutral thermolysin-like-protease from Geobacillus stearothermophilus , and wherein said stain comprises a protein.

10. A composition for facilitating biological stain removal comprising:
a liquid bioactive coating material comprising an alcohol selected from the group consisting of methanol, ethanol, isopropyl alcohol, propanol and butanol, wherein the concentration of said alcohol in said liquid coating is from 20 to 40% wt. %, wherein the pH of the liquid coating is grater than 5.0; and
a thermolysin-like protease, wherein the protease has an activity in excess of 20,000 U/mg, said protease capable of degrading a biological stain component.

11. The composition of claim 10 wherein said thermolysin-like protease is an active extracellular portion of bacterial neutral thermolysin-like-protease from Geobacillus stearothermophilus.

12. The composition of claim 10 wherein the pH of said liquid bioactive coating material is greater than 8.0.

13. The composition of claim 10 wherein said protease is in solution in said liquid bioactive coating material.

14. The method of claim 1 wherein said alcohol is methanol, and said stain is an insect stain.

15. The composition of claim 10 wherein said alcohol is methanol, and said stain is an insect stain.

16. The composition of claim 15 wherein, said thermolysin-like protease is an extracellular fragment of a bacterial neutral thermolysin-like-protease from Geobacillus stearothermophilus , and wherein said liquid coating material has a pH of 8.0 or greater.

17. The composition of claim 10 , wherein said thermolysin-like protease is an extracellular fragment of a bacterial neutral thermolysin-like-protease from Geobacillus stearothermophilus , and wherein said stain comprises a protein.

Assignees

• Toyota Eng & Mfg North America
• Toyota Motor Corp

Inventors

• Wu Songtao
• Jia Hongfei
• Ishii Masahiko
• Zhang Minjuan

Classifications

• C09D189/00

  Coating compositions based on proteins; Coating compositions based on derivatives thereof (foodstuff preparations A23J3/00) · CPC title

• C09D5/1637

  Macromolecular compounds · CPC title

• C12Y304/24027

  Thermolysin (3.4.24.27) · CPC title

• C11D7/04

  Water-soluble compounds · CPC title

• C08L33/00

  Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides or nitriles thereof; Compositions of derivatives of such polymers · CPC title