Polypeptides

US12129497B2 · US · B2

Patent metadata
FieldValue
Publication numberUS-12129497-B2
Application numberUS-202218053614-A
CountryUS
Kind codeB2
Filing dateNov 8, 2022
Priority dateOct 7, 2015
Publication dateOct 29, 2024
Grant dateOct 29, 2024

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  1. Title

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  2. Abstract

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  3. Assignees and inventors

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  4. Key dates

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  5. First independent claim

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  6. CPC / IPC classifications

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  7. Citations and related patents

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Abstract

Official abstract text for this publication.

The present invention relates to polypeptides, nucleotides encoding the polypeptide, as well as methods of producing the polypeptides. The present invention also relates to detergent compositions comprising polypeptides, a laundering method and the use of polypeptides.

First claim

Opening claim text (preview).

The invention claimed is: 1. A recombinant microbial host cell transformed with a polynucleotide encoding a polypeptide having DNase activity, wherein (a) the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in the recombinant microbial host cell, and (b) the amino acid sequence of the polypeptide has at least 85% sequence identity to the amino acid sequence of SEQ ID NO: 5. 2. A method of producing a polypeptide having DNase activity, comprising cultivating the recombinant microbial host cell of claim 1 under conditions conducive for production of the polypeptide. 3. The method of claim 2 , wherein the amino acid sequence of the polypeptide has at least 90% sequence identity to SEQ ID NO: 5. 4. The method of claim 2 , wherein the amino acid sequence of the polypeptide has at least 95% sequence identity to SEQ ID NO: 5. 5. The method of claim 2 , wherein the recombinant microbial host cell is a Gram-positive bacterium selected from the group consisting of Bacillus, Clostridium, Enterococcus, Geobacillus, Lactobacillus, Lactococcus, Oceanobacillus, Staphylococcus, Streptococcus , and Streptomyces. 6. The method of claim 2 , wherein the recombinant microbial host cell is a Bacillus host cell. 7. A recombinant microbial host cell comprising a polynucleotide encoding a polypeptide having DNase activity, wherein (a) the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in the recombinant microbial host cell, wherein at least one of the one or more control sequences is heterologous to the polynucleotide, and (b) the amino acid sequence of the polypeptide has at least 85% sequence identity to the amino acid sequence of SEQ ID NO: 5. 8. A method of producing a polypeptide having DNase activity, comprising cultivating the recombinant microbial host cell of claim 7 under conditions conducive for production of the polypeptide. 9. The method of claim 8 , wherein the amino acid sequence of the polypeptide has at least 90% sequence identity to SEQ ID NO: 5. 10. The method of claim 8 , wherein the amino acid sequence of the polypeptide has at least 95% sequence identity to SEQ ID NO: 5. 11. The method of claim 8 , wherein the recombinant microbial host cell is a Gram-positive bacterium selected from the group consisting of Bacillus, Clostridium, Enterococcus, Geobacillus, Lactobacillus, Lactococcus, Oceanobacillus, Staphylococcus, Streptococcus , and Streptomyces. 12. The method of claim 8 , wherein the recombinant microbial host cell is a Bacillus host cell. 13. A recombinant microbial host cell comprising a polynucleotide encoding a polypeptide having DNase activity, wherein (a) the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in the recombinant host cell, wherein the polynucleotide is heterologous to the recombinant microbial host cell, and (b) the amino acid sequence of the polypeptide has at least 85% sequence identity to the amino acid sequence of SEQ ID NO: 5. 14. A method of producing a polypeptide having DNase activity, comprising cultivating the recombinant microbial host cell of claim 13 under conditions conducive for production of the polypeptide. 15. The method of claim 14 , wherein the amino acid sequence of the polypeptide has at least 90% sequence identity to SEQ ID NO: 5. 16. The method of claim 14 , wherein the amino acid sequence of the polypeptide has at least 95% sequence identity to SEQ ID NO: 5. 17. The method of claim 14 , wherein the recombinant microbial host cell is a Gram-positive bacterium selected from the group consisting of Bacillus, Clostridium, Enterococcus, Geobacillus, Lactobacillus, Lactococcus, Oceanobacillus, Staphylococcus, Streptococcus , and Streptomyces. 18. The method of claim 14 , wherein the recombinant microbial host cell is a Bacillus host cell.

Assignees

Inventors

Classifications

  • Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21) · CPC title

  • Soft surfaces, e.g. textile · CPC title

  • containing lipase · CPC title

  • Polyethers, e.g. polyalkyleneoxides · CPC title

  • Carbohydrates or derivatives thereof · CPC title

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Frequently asked questions

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What does patent US12129497B2 cover?
The present invention relates to polypeptides, nucleotides encoding the polypeptide, as well as methods of producing the polypeptides. The present invention also relates to detergent compositions comprising polypeptides, a laundering method and the use of polypeptides.
Who is the assignee on this patent?
Novozymes As
What technology area does this patent fall under?
Primary CPC classification C12N9/22. Mapped technology areas include Chemistry & Metallurgy.
When was this patent published?
Publication date Tue Oct 29 2024 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).