Polypeptides

Patent metadata

Field	Value
Publication number	US-10954497-B2
Application number	US-201615766894-A
Country	US
Kind code	B2
Filing date	Oct 7, 2016
Priority date	Oct 7, 2015
Publication date	Mar 23, 2021
Grant date	Mar 23, 2021

Abstract

The present invention relates to polypeptides, nucleotides encoding the polypeptide, as well as methods of producing the polypeptides. The present invention also relates to detergent composition comprising polypeptides, a laundering method and the use of polypeptides.

First claim

The invention claimed is:

1. A composition comprising
(a) at least 0.002 ppm of a polypeptide having DNase activity, wherein the polypeptide comprises the motif HXXP and has at least 95% sequence identity to the polypeptide shown in SEQ ID NO: 21,
(b) one or more polyol(s) selected from the group consisting of glycerol, (mono, di, or tri) propylene glycol, ethylene glycol, polyethylene glycol, sugar alcohols, sorbitol, mannitol, erythritol, dulcitol, inositol, xylitol and adonitol, and
(c) a surfactant,

wherein the composition is formulated as a bar, a homogenous tablet, and a tablet having two or more layers, a pouch having one or more compartments, a regular or compact powder, a granule, a paste, a gel, or a liquid.

2. The composition of claim 1 , wherein the polypeptide having DNase activity has at least 97% sequence identity to the polypeptide shown in SEQ ID NO: 21.

3. The composition of claim 1 , wherein the polypeptide having DNase activity comprises the amino acid sequence of SEQ ID NO: 21.

4. The composition of claim 1 , wherein the polypeptide having DNase activity consists of the amino acid sequence of SEQ ID NO: 21.

5. The composition of claim 1 , wherein the polypeptide having DNase activity is a variant of the polypeptide of SEQ ID NO: 21, wherein the variant has DNase activity and comprises one or more amino acid substitutions, or one or more amino acid deletions, or one or more amino acid insertions or any combination thereof in 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 positions.

6. The composition of claim 1 , wherein the polypeptide having DNase activity comprises one or more of the motifs selected from the group consisting of

(SEQ ID NO 198)

[T/D/S][G/N]PQL,

(SEQ ID NO 199)

[G/T]Y[D/S][R/K/L],

(SEQ ID NO 200)

[E/D/H]H[I/V/L/F/M]X[P/A/S],

(SEQ ID NO: 201)

[F/L/Y/I]A[N/R]D[L/I/P/V]

and

(SEQ ID NO: 202)

C[D/N]T[A/R].

7. The composition of claim 1 , wherein the polypeptide having DNase activity comprises a GYS motif and one or both of the motifs [D/M/L][S/T]GYSR[D/N] (SEQ ID NO: 204) or ASXNRSKG (SEQ ID NO: 205).

8. The composition of claim 1 , further comprising a second enzyme.

9. The composition of claim 8 , wherein the second enzyme is a protease.

10. The composition of claim 8 , wherein the second enzyme is an amylase.

11. The composition of claim 8 , wherein the second enzyme is a lipase.

12. The composition of claim 1 , wherein the surfactant is an anionic or a nonionic surfactant.

13. The composition of claim 1 , further comprising a polymer.

14. The composition of claim 1 , wherein the composition is in the form of a granule comprising a core and a coating that has one or more layer(s) surrounding the core.

15. The composition of claim 1 , wherein the composition is in the form of a liquid.

Assignees

• Novozymes As

Inventors

• Gjermansen Morten
• Gori Klaus
• Geertz-Hansen Henrik Marcus
• Salomon Jesper
• Blicher Thomas Holberg
• Stringer Mary Ann
• Spodsberg Nikolaj
• Sun tianqi

Classifications

• C12Y301/21

  Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21) · CPC title

• C11D3/22

  Carbohydrates or derivatives thereof · CPC title

• C11D3/2065

  Polyhydric alcohols · CPC title

• C11D3/38681

  Chemically modified or immobilised enzymes · CPC title

• C11D3/38636

  containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase · CPC title