Polypeptides

Patent metadata

Field	Value
Publication number	US-10844360-B2
Application number	US-201615737169-A
Country	US
Kind code	B2
Filing date	Oct 7, 2016
Priority date	Oct 7, 2015
Publication date	Nov 24, 2020
Grant date	Nov 24, 2020

Abstract

The present invention relates to polypeptides, nucleotides encoding the polypeptide, as well as methods of producing the polypeptides. The present invention also relates to detergent composition comprising polypeptides, a laundering method and the use of polypeptides.

First claim

The invention claimed is:

1. A detergent composition comprising a polypeptide having DNase activity and an effective amount of a surfactant to improve wash performance of the detergent composition as compared to the same detergent composition without the effective amount of the surfactant, wherein
the amino acid sequence of the polypeptide having DNase activity has at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 1,
the composition is a bar, a homogenous tablet, a tablet having two or more layers, a pouch having one or more compartments, a regular or compact powder, a granule, a paste, a gel, or a regular, compact or concentrated liquid, and
wherein the detergent composition reduces soil deposition onto a Brevundiomonas biofilm swatch in a wash assay as compared to the amount of soil deposition by the same detergent composition without said polypeptide having DNase activity.

2. The detergent composition of claim 1 , wherein the amino acid sequence of the polypeptide having DNase activity has at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 1.

3. The detergent composition of claim 1 , wherein the amino acid sequence of the polypeptide having DNase activity has at least 97% sequence identity to the amino acid sequence of SEQ ID NO: 1.

4. The detergent composition of claim 1 , wherein the amino acid sequence of the polypeptide having DNase activity comprises the amino acid sequence of SEQ ID NO: 1.

5. The detergent composition of claim 1 , wherein the polypeptide having DNase activity is a variant of the amino acid sequence of SEQ ID NO: 1, wherein the amino acid sequence of the variant comprises a substitution, deletion, and/or insertion at one or more positions relative to the amino acid sequence of SEQ ID NO: 1.

6. The detergent composition of claim 1 , wherein the polypeptide having DNase activity is a fragment of the amino acid sequence of SEQ ID NO: 1, wherein the fragment has DNase activity.

7. The detergent composition of claim 1 , further comprising a detergent adjunct ingredient selected from the group consisting of builders, flocculating aid, chelating agents, dye transfer inhibitors, enzymes, enzyme stabilizers, enzyme inhibitors, catalytic materials, bleach activators, hydrogen peroxide, sources of hydrogen peroxide, preformed peracids, polymeric dispersing agents, clay soil removal/anti-redeposition agents, brighteners, suds suppressors, dyes, perfumes, structure elasticizing agents, fabric softeners, carriers, hydrotropes, builders, cobuilders, fabric huing agents, anti-foaming agents, dispersants, processing aids, and pigments.

8. The detergent composition of claim 1 , further comprising one or more enzymes selected from the group consisting of amylases, arabinases, carbohydrases, cellulases, cutinases, galactanases, lipases, mannanases, oxidases, pectinases, proteases, and xylanases.

9. The detergent composition of claim 8 , wherein the one or more enzymes comprises a protease.

10. The detergent composition of claim 9 , wherein the protease is a serine protease.

11. A method for laundering a textile, comprising:
(a) exposing the textile to a wash liquor comprising the detergent composition of claim 1 ; and
(b) completing at least one wash cycle.

12. The method of claim 11 , further comprising rinsing the textile.

13. The method of claim 11 , wherein the concentration of the polypeptide having DNase activity in the wash liquor is at least 1 mg per liter of wash liquor.

14. The method of claim 11 , wherein the pH of the wash liquor is in the range 5.5 to 11.

15. The method of claim 11 , wherein the temperature of the wash liquor is in the range of 5 to 95° C.

16. The method of claim 11 , wherein the textile is made of cotton, polyacryl, polyamide, polyester, and/or silk.

17. The method of claim 11 , further comprising a second wash cycle.

18. The detergent composition of claim 1 , wherein the detergent composition is a regular, compact or concentrated liquid, and wherein the surfactant is present in an amount of 2 wt % to 60 wt % of the detergent composition.

19. The detergent composition of claim 1 , wherein the detergent composition is a granule comprising 5 wt % to 50 wt % anionic surfactant and 1 wt % to 8 wt % nonionic surfactant.

20. The detergent composition of claim 1 , wherein the surfactant is selected from the group consisting of alkyl ether sulphates, C9-C15 alcohol ethersulfates, C12-C15 primary alcohol ethoxylate, C8-C16 ester sulphates and C10-C14 ester sulphates, mono dodecyl ester sulphates, sulfates, sulfonates, linear alkylbenzenesulfonates (LAS), isomers of LAS, branched alkylbenzenesulfonates (BABS), phenylalkanesulfonates, alpha-olefinsulfonates (AOS), olefin sulfonates, alkene sulfonates, alkane-2,3-diylbis(sulfates), hydroxyalkanesulfonates, disulfonates, alkyl sulfates (AS), sodium dodecyl sulfate (SDS), fatty alcohol sulfates (FAS), primary alcohol sulfates (PAS), alcohol ethersulfates (AEOS), secondary alkanesulfonates (SAS), paraffin sulfonates (PS), ester sulfonates, sulfonated fatty acid glycerol esters, alpha-sulfo fatty acid methyl esters, methyl ester sulfonate (MES), alkyl- or alkenylsuccinic acid, dodecenyl/tetradecenyl succinic acid (DTSA), fatty acid derivatives of amino acids, diesters and monoesters of sulfo-succinic acid or salt of fatty acids (soap), and combinations thereof.

21. The detergent composition of claim 1 , wherein the surfactant is LAS or AEOS, and wherein the surfactant is present in an amount of 1% to 15% of the detergent composition.

Assignees

• Novozymes As

Inventors

• Gjermansen Morten
• Gori Klaus
• Geertz-Hansen Henrik Marcus
• Salomon Jesper
• Stringer Mary Ann
• Blicher Thomas Holberg
• Spodsberg Nikolaj

Classifications

• C12N9/22 Primary

  Ribonucleases {[RNase]; Deoxyribonucleases [DNase]} · CPC title

• C11D3/3707

  Polyethers, e.g. polyalkyleneoxides · CPC title

• C11D3/38636

  containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase · CPC title

• C11D3/38681

  Chemically modified or immobilised enzymes · CPC title

• C11D3/22

  Carbohydrates or derivatives thereof · CPC title