Systems and methods for cell culture device interconnection and fluidic device interconnection

Patent metadata

Field	Value
Publication number	US-12065634-B2
Application number	US-202217871054-A
Country	US
Kind code	B2
Filing date	Jul 22, 2022
Priority date	Jul 12, 2013
Publication date	Aug 20, 2024
Grant date	Aug 20, 2024

Abstract

Systems and methods interconnect cell culture devices and/or fluidic devices by transferring discrete volumes of fluid between devices. A liquid-handling system collects a volume of fluid from at least one source device and deposits the fluid into at least one destination device. In some embodiments, a liquid-handling robot actuates the movement and operation of a fluid collection device in an automated manner to transfer the fluid between the at least one source device and the at least one destination device. In some cases, the at least one source device and the at least one destination device are cell culture devices. The at least one source device and the at least one destination device may be microfluidic or non-microfluidic devices. In some cases, the cell culture devices may be microfluidic cell culture devices. In further cases, the microfluidic cell culture devices may include organ-chips.

First claim

What is claimed is:

1. A method of transferring fluid, the method comprising:
a) providing a system comprising (i) a microfluidic cell culture device comprising a microfluidic channel connected to an input port and an output port, said microfluidic channel comprising cells, (ii) a fluid collection device coupled to a movement system that is configured to move the fluid collection device into a desired position relative to at least said microfluidic cell culture device, and (iii) a perfusion mechanism,
b) moving the fluid collection device to a first desired position relative to the output port of said microfluidic cell culture device;
c) collecting a first discrete fluid volume from said output port of the first microfluidic cell culture device;
d) moving the fluid collection device to a second desired position relative to the input port of said microfluidic cell culture device;
e) depositing a second discrete fluid volume comprising at least a portion of the first discrete fluid volume into said input port; and
f) perfusing, using said a perfusion means mechanism, the respective second discrete fluid volume through said microfluidic channel of said microfluidic cell culture device.

2. The method of claim 1 , wherein said microfluidic channel has at least one dimension that is less than or equal to approximately 3 mm.

3. The method of claim 1 , wherein said fluid collection device includes a tip that accesses the microfluidic cell culture device.

4. The method of claim 1 , wherein the input port includes a port structure, the port structure including a chamber for receiving the output fluid.

5. The method of claim 1 , wherein the port structure includes a pressure relief mechanism that relieves the pressure in the chamber generated by the fluid channel receiving the output fluid.

Assignees

• Harvard College

Inventors

• Ingber Donald E
• Levner Daniel
• Thompson Ii Guy
• Fernandez-Alcon Jose
• Hinojosa Christopher David

Classifications

• C12M41/48

  Automatic or computerized control (automatic analysis G01N35/00) · CPC title

• C12M37/04

  Seals · CPC title

• C12M29/20

  Degassing; Venting; Bubble traps (means for collection or storage of gas C12M23/36; gas collection apparatus for laboratory use B01L5/02) · CPC title

• C12M23/38

  Caps; Covers; Plugs; Pouring means · CPC title

• C12M21/08

  for producing artificial tissue or for ex-vivo cultivation of tissue (prostheses A61F2/00, grafts A61L27/00) · CPC title