Systems and methods for cell culture device interconnection and fluidic device interconnection

Patent metadata

Field	Value
Publication number	US-10465158-B2
Application number	US-201414904640-A
Country	US
Kind code	B2
Filing date	Jul 11, 2014
Priority date	Jul 12, 2013
Publication date	Nov 5, 2019
Grant date	Nov 5, 2019

Abstract

Systems and methods interconnect cell culture devices and/or fluidic devices by transferring discrete volumes of fluid between devices. A liquid-handling system collects a volume of fluid from at least one source device and deposits the fluid into at least one destination device. In some embodiments, a liquid-handling robot actuates the movement and operation of a fluid collection device in an automated manner to transfer the fluid between the at least one source device and the at least one destination device. In some cases, the at least one source device and the at least one destination device are cell culture devices. The at least one source device and the at least one destination device may be microfluidic or non-microfluidic devices. In some cases, the cell culture devices may be microfluidic cell culture devices. In further cases, the microfluidic cell culture devices may include organ-chips.

First claim

What is claimed is:

1. A method for transferring fluid from a first cell culture device to a second cell culture device, the method comprising:
moving a fluid collection device to a first desired position relative to a source device, said source device comprising a first cell culture microfluidic device, said first cell culture microfluidic device comprising biologic material and a port, said port connected to a reservoir of media, said media used to maintain the viability of said biologic material;
collecting a first discrete fluid droplet from the first cell culture device;
moving the fluid collection device to a second desired position relative to a destination device, said destination device comprising a second cell culture microfluidic device comprising biologic material; and
depositing said first discrete fluid droplet into the second cell culture microfluidic device; and perfusing the biologic material of the destination device with the first discrete fluid droplet.

2. The method according to claim 1 , wherein the second cell culture microfluidic device includes a chamber configured to hold the first discrete fluid droplet and the fluid collection device deposits the first discrete fluid droplet into the chamber, respectively.

3. The method according to claim 1 , further comprising:
moving said fluid collection device back to said first desired position relative to said source device, said source device comprising said first cell culture microfluidic device;
collecting a second discrete fluid droplet from the first cell culture device;
moving the fluid collection device to a third desired position relative to a third cell culture microfluidic device; and
depositing said second discrete fluid droplet into the third cell culture device.

4. The method according to claim 1 , wherein the first cell culture device includes a first output port, the fluid collection device accessing the first output port to collect the first discrete fluid droplet, the first desired position being determined by the first output port, and the second cell culture device includes a second input port, the fluid collection device accessing the second input port to deposit the first discrete fluid droplet, the second desired position being determined by the second input port.

5. The method according to claim 1 , wherein the fluid collection device includes an elongated chamber, capillary channel, or a microfluidic channel that receives a plurality of samples of fluid over a period of time, each sample of fluid substantially maintaining at least one physical, chemical, or biochemical characteristic from a time the sample was collected, the plurality of samples providing time-based information regarding the at least one physical, chemical, or biochemical characteristic.

6. The method according to claim 4 , wherein the output port includes a port structure, the port structure including a chamber for receiving the first discrete fluid droplet to be collected by the fluid collection device.

7. The method according to claim 6 , wherein the first cell culture device includes a fluid channel coupled to the chamber and providing the first discrete fluid droplet to the chamber.

8. The method according to claim 6 , wherein the port structure includes a pressure relief mechanism that relieves the positive pressure in the chamber generated by the fluid channel providing the respective first discrete fluid droplet to the chamber.

9. The method according to claim 4 , wherein the input port includes a port structure, the port structure including a chamber for receiving the second discrete fluid droplet from the fluid collection device.

10. The method according to claim 9 , wherein the second cell culture device includes a fluid channel coupled to the chamber and receiving the first discrete fluid droplet from the chamber.

11. The method according to claim 9 , wherein the port structure includes a pressure relief mechanism that relieves the negative pressure in the chamber generated by the fluid channel receiving the first discrete fluid droplet from the chamber.

12. The method according to claim 4 , wherein the output port or the input port includes a port structure, the port structure including a chamber for storing fluid that is to be collected by the fluid collection device or that is received from the fluid collection device, the chamber being covered to minimize contamination and fluid evaporation in the chamber.

13. The method of claim 1 , wherein a liquid-handling robot actuates said moving of said fluid collection device in an automated manner to transfer the first discrete fluid droplet from said source device to said destination device.

Assignees

• Harvard College

Inventors

• Ingber Donald E
• Levner Daniel
• Thompson Ii Guy
• Fernandez-Alcon Jose
• Hinojosa Christopher David

Classifications

• C12M33/04

  by injection or suction, e.g. using pipettes, syringes, needles (pipettes in general B01L3/02) · CPC title

• B01L3/0293

  for liquids · CPC title

• C12M29/00

  Means for introduction, extraction or recirculation of materials, e.g. pumps (pumps per se F04B) · CPC title

• C12M23/58 Primary

  Reaction vessels connected in series or in parallel (combinations of bioreactors with other apparatus, C12M43/00) · CPC title

• B01L2200/027

  for microfluidic devices · CPC title