Systems and methods for cell culture device interconnection and fluidic device interconnection

Patent metadata

Field	Value
Publication number	US-11499131-B2
Application number	US-201916593646-A
Country	US
Kind code	B2
Filing date	Oct 4, 2019
Priority date	Jul 12, 2013
Publication date	Nov 15, 2022
Grant date	Nov 15, 2022

Abstract

Systems and methods interconnect cell culture devices and/or fluidic devices by transferring discrete volumes of fluid between devices. A liquid-handling system collects a volume of fluid from at least one source device and deposits the fluid into at least one destination device. In some embodiments, a liquid-handling robot actuates the movement and operation of a fluid collection device in an automated manner to transfer the fluid between the at least one source device and the at least one destination device. In some cases, the at least one source device and the at least one destination device are cell culture devices. The at least one source device and the at least one destination device may be microfluidic or non-microfluidic devices. In some cases, the cell culture devices may be microfluidic cell culture devices. In further cases, the microfluidic cell culture devices may include organ-chips.

First claim

What is claimed is:

1. A method for facilitating biological communication between two or more microfluidic cell culture devices by transferring fluid from a first microfluidic cell culture device to a second microfluidic cell culture device, the method comprising:
providing a system comprising (a) the first microfluidic cell culture device having an output port, (b) the second microfluidic cell culture device having an input port, (c) a fluid collection device coupled to a movement system that is configured to move the fluid collection device into a desired position relative to at least one of the first cell culture device and the second cell culture device, and (d) a perfusion mechanism,
moving the fluid collection device to a first desired position relative to the first microfluidic cell culture device comprising a microfluidic channel including a first organ tissue;
collecting a first discrete fluid volume from said output port of the first microfluidic cell culture device;
moving the fluid collection device to a second desired position relative to the second microfluidic cell culture device comprising a microfluidic channel including a second organ tissue;
depositing a second discrete fluid volume comprising at least a portion of the first discrete fluid volume into said input port of the second microfluidic cell culture device; and
perfusing, using said perfusion mechanism, the respective second discrete fluid volume through the second microfluidic cell culture device.

2. The method according to claim 1 wherein the first microfluidic cell culture device or the second microfluidic cell culture device includes a chamber configured to hold correspondingly the respective first discrete fluid volume or the respective second discrete fluid volume, the desired position is determined according to the chamber, and the fluid collection device correspondingly collects the respective first discrete fluid volume from or deposits the respective second discrete fluid volume into the chamber.

3. The method according to claim 1 wherein the output port includes a port structure, the port structure including a chamber for receiving the respective first discrete fluid volume to be collected by the fluid collection device.

4. The method according to claim 1 wherein the input port includes a port structure, the port structure including a chamber for receiving the respective second discrete fluid volume from the fluid collection device.

5. The method according to claim 1 wherein the output port or the input port includes a port structure, the port structure including a chamber for storing fluid that is to be collected by the fluid collection device or that is received from the fluid collection device, the chamber being covered to minimize contamination and fluid evaporation in the chamber.

6. The method according to claim 1 wherein the fluid collection device includes a collection chamber selected from the group consisting of an elongated chamber, capillary channel, or a microfluidic channel that receives a plurality of samples of fluid over a period of time, each sample of fluid substantially maintaining at least one physical, chemical, or biochemical characteristic from a time the sample was collected, the plurality of samples providing time-based information regarding the at least one physical, chemical, or biochemical characteristic.

7. The method according to claim 1 further comprising:
moving the fluid collection device to a third desired position relative to a third microfluidic cell culture device; and
depositing a third discrete fluid volume into a port of the third microfluidic cell culture device,
wherein the third discrete fluid volume includes at least another portion of the first discrete fluid volume collected from the first microfluidic cell culture device.

Assignees

• Harvard College

Inventors

• Ingber Donald E
• Levner Daniel
• Thompson Ii Guy
• Fernandez-Alcon Jose
• Hinojosa Christopher David

Classifications

• C12M23/38

  Caps; Covers; Plugs; Pouring means · CPC title

• C12M37/04

  Seals · CPC title

• C12M33/04

  by injection or suction, e.g. using pipettes, syringes, needles (pipettes in general B01L3/02) · CPC title

• B01L3/0293

  for liquids · CPC title

• C12M29/00

  Means for introduction, extraction or recirculation of materials, e.g. pumps (pumps per se F04B) · CPC title