Rust resistance gene

Patent metadata

Field	Value
Publication number	US-12037595-B2
Application number	US-202217865137-A
Country	US
Kind code	B2
Filing date	Jul 14, 2022
Priority date	Aug 21, 2013
Publication date	Jul 16, 2024
Grant date	Jul 16, 2024

Abstract

The present invention relates to new transporter polypeptides, and genes encoding therefor, which can be used to confer upon a plant resistance to one or more biotrophic fungal pathogens.

First claim

The invention claimed is:

1. A genetically modified plant comprising a gene encoding an Lr67 polypeptide which comprises amino acids having a sequence at least 80% identical to the amino acid sequence of SEQ ID NO:1, wherein the gene has a genetic modification such that the polypeptide does not comprise a glycine at a position corresponding to amino acid number 144 of SEQ ID NO:1.

2. The genetically modified plant of claim 1 , wherein when expressed in the plant, the Lr67 polypeptide confers upon the plant resistance to one or more biotrophic fungal pathogen(s).

3. The genetically modified plant of claim 2 , wherein the one or more biotrophic fungal pathogen(s) is selected from the group consisting of Blumeria graminis f. sp. tritici, Fusarium graminearum, Bipolaris sorokiniana, Erysiphe graminis f. sp. tritici, Puccinia graminis f sp. tritici, Puccinia striiformis, Puccinia hordei and Puccinia recondite f. sp. tritici.

4. The genetically modified plant of claim 1 , wherein the Lr67 polypeptide comprises an amino acid sequence which is at least 85% identical to the amino acid sequence of SEQ ID NO:1.

5. The genetically modified plant of claim 1 which has enhanced resistance to one or more biotrophic fugal pathogen(s) when compared to an isogenic plant lacking the genetic modification.

6. The genetically modified plant of claim 1 which has enhanced resistance to one or more biotrophic fungal pathogen(s) at the seedling stage of growth.

7. The genetically modified plant of claim 1 which is a cereal, grape, beet or leguminous plant.

8. A seed of the genetically modified plant of claim 1 which comprises the gene.

9. A method of producing a genetically modified plant, the method comprising the steps of
i) genetically modifying a cell of a plant such that it comprises a gene encoding an Lr67 polypeptide which comprises amino acids having a sequence at least 80% identical to the amino acid sequence of SEQ ID NO:1, wherein the gene has a genetic modification such that the polypeptide does not comprise a glycine at a position corresponding to amino acid number 144 of SEQ ID NO:1, and
ii) regenerating a transgenic plant from the cell,

thereby producing the transgenic plant.

10. A method of producing a plant part, the method comprising,
a) growing a plant of claim 1 , and
b) harvesting the plant part.

11. A method of producing flour, wholemeal, starch or other product obtained from seed, the method comprising:
a) obtaining seed of claim 8 , and
b) extracting the flour, wholemeal, starch or other product.

12. A product produced from the plant of claim 1 or seed thereof, wherein the product is a food product or beverage product which comprises the gene.

13. An isolated and/or exogenous polynucleotide comprising nucleotides having a sequence as provided in SEQ ID NO:2 or SEQ ID NO:3, or a sequence which is at least 80% identical to one or both of SEQ ID NO:2 and SEQ ID NO:3, wherein the polynucleotide encodes an Lr67 polypeptide which does not comprise a glycine at a position corresponding to amino acid number 144 of SEQ ID NO:1, and wherein the polynucleotide is operably linked to a heterologous promoter.

14. A chimeric vector comprising the polynucleotide of claim 13 .

15. A method of producing a genetically modified plant, the method comprising the steps of
i) introducing a polynucleotide of claim 13 into a cell of a plant, and
ii) regenerating a genetically modified plant from the cell,

thereby producing the transgenic plant.

16. A method of selecting a genetically modified plant, the method comprising the steps of
i) screening one or more progeny plants from the cross of two parental plants for the presence or absence of a polynucleotide of claim 13 , wherein at least one plant comprises the polynucleotide, and
ii) selecting a progeny plant which comprises the polynucleotide,

thereby selecting the genetically modified plant.

Assignees

• Commw Scient Ind Res Org

Inventors

• Lagudah Evans
• Moore John Wallace

Classifications

• G01N2333/415

  from plants · CPC title

• C12Q2600/158

  Expression markers · CPC title

• C12Q2600/13

  Plant traits · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12Q1/54

  involving glucose or galactose · CPC title