Rust resistance gene

Patent metadata

Field	Value
Publication number	US-10647992-B2
Application number	US-201815970733-A
Country	US
Kind code	B2
Filing date	May 3, 2018
Priority date	Aug 21, 2013
Publication date	May 12, 2020
Grant date	May 12, 2020

Abstract

The present invention relates to new transporter polypeptides, and genes encoding therefor, which can be used to confer upon a plant resistance to one or more biotrophic fungal pathogens.

First claim

The invention claimed is:

1. A transgenic plant which has integrated into its genome a transgene comprising an exogenous polynucleotide encoding an Lr67 polypeptide which comprises amino acids having a sequence at least 90% identical to the amino acid sequence of any one or more of SEQ ID NO:1, SEQ ID NO:7, SEQ ID NO:8 or SEQ ID NO:9, wherein the Lr67 polypeptide does not comprise a glycine at a position corresponding to amino acid number 144 of SEQ ID NO:1, and wherein the polynucleotide is operably linked to a promoter capable of directing expression of the polynucleotide in a cell of the plant.

2. The transgenic plant of claim 1 , wherein when expressed in the plant, the Lr67 polypeptide confers upon the plant resistance to one or more biotrophic fungal pathogen(s).

3. The transgenic plant of claim 2 , wherein the one or more biotrophic fungal pathogen(s) is selected from the group consisting of Blumeria graminis f. sp. tritici, Fusarium graminearum, Bipolaris sorokiniana, Erysiphe graminis f. sp. tritici, Puccinia graminis f. sp. tritici, Puccinia striiformis, Puccinia hordei and Puccinia recondita f. sp. tritici.

4. The transgenic plant of claim 1 , wherein the Lr67 polypeptide comprises amino acids having a sequence at least 95% identical to the amino acid sequence of any one or more of SEQ ID NO:1, SEQ ID NO:7, SEQ ID NO:8 or SEQ ID NO:9.

5. The transgenic plant of claim 1 which has enhanced resistance to one or more biotrophic fungal pathogen(s) when compared to an isogenic plant lacking the exogenous polynucleotide.

6. The transgenic plant of claim 1 which has enhanced resistance to one or more biotrophic fungal pathogen(s) at the seedling stage of growth.

7. The transgenic plant of claim 1 which comprises one or more further exogenous polynucleotides encoding a plant pathogen resistance polypeptide other than an Lr67 polypeptide.

8. The transgenic plant of claim 1 which is a cereal plant.

9. The transgenic plant of claim 8 which is a wheat plant.

10. The transgenic plant of claim 1 which is homozygous for the exogenous polynucleotide.

11. The transgenic plant of claim 1 which is a cereal, grape, beet or leguminous plant.

12. A process for determining whether a polypeptide confers resistance or susceptibility to one or more biotrophic fungal pathogen(s), comprising:
i) obtaining a polynucleotide operably linked to a promoter, the polynucleotide encoding an Lr67 polypeptide which comprises amino acids having a sequence at least 90% identical to the amino acid sequence of any one or more of SEQ ID NO:1, SEQ ID NO:7, SEQ ID NO:8 or SEQ ID NO:9, wherein the Lr67 polypeptide does not comprise a glycine at a position corresponding to amino acid number 144 of SEQ ID NO:1,
ii) introducing the polynucleotide into a plant,
iii) determining whether the level of resistance or susceptibility to the one or more biotrophic fungal pathogen(s), is increased or decreased relative to an isogenic plant lacking the polynucleotide, and
iv) optionally, if the level of resistance or susceptibility is increased, selecting a polynucleotide encoding the polypeptide which when expressed confers resistance or susceptibility to the one or more biotrophic fungal pathogen(s).

13. The process of claim 12 , wherein one or more of the following apply,
a) the plant is a cereal, grape, beet or leguminous plant,
b) the polypeptide is a plant polypeptide or mutant thereof, and
c) step ii) further comprises stably integrating the polynucleotide operably linked to a promoter into the genome of the plant.

14. The process of claim 12 , wherein the biotrophic fungal pathogen(s) is a rust, a mildew or both a rust and a mildew.

15. The process of claim 12 , wherein the biotrophic fungal pathogen(s) is one or more or all of leaf rust, stripe rust, stem rust and powdery mildew.

16. A plant seed of a plant of claim 1 , which comprises the Lr67 polypeptide.

17. A method of producing a plant part, the method comprising,
a) growing a plant of claim 1 , and
b) harvesting the plant part.

18. A method of producing flour, wholemeal, starch or other product obtained from seed, the method comprising;
a) obtaining seed of claim 16 , and
b) extracting the flour, wholemeal, starch or other product.

Assignees

• Commw Scient Ind Res Org
• Grains Res & Dev Corp

Inventors

• Lagudah Evans
• Moore John Wallace

Classifications

• A01N65/08

  Magnoliopsida [dicotyledons] · CPC title

• C12Q2600/13

  Plant traits · CPC title

• C12Q1/54

  involving glucose or galactose · CPC title

• C07K14/415

  from plants · CPC title

• C12N15/8282 Primary

  for fungal resistance · CPC title