Rust resistance gene

Patent metadata

Field	Value
Publication number	US-9994864-B2
Application number	US-201414912874-A
Country	US
Kind code	B2
Filing date	Aug 21, 2014
Priority date	Aug 21, 2013
Publication date	Jun 12, 2018
Grant date	Jun 12, 2018

Abstract

The present invention relates to new transporter polypeptides, and genes encoding therefor, which can be used to confer upon a plant resistance to one or more biotrophic fungal pathogens.

First claim

The invention claimed is:

1. A transgenic plant which has integrated into its genome a transgene comprising an exogenous polynucleotide encoding an Lr67 polypeptide which comprises amino acids having a sequence at least 95% identical to the amino acid sequence of SEQ ID NO:1, wherein the Lr67 polypeptide does not comprise a glycine at a position corresponding to amino acid number 144 of SEQ ID NO:1, and wherein the polynucleotide is operably linked to a promoter capable of directing expression of the polynucleotide in a cell of the plant.

2. The transgenic plant of claim 1 , wherein the transgenic plant comprises one or more or all of:
i) when expressed in the plant, the Lr67 polypeptide confers upon the plant resistance to one or more biotrophic fungal pathogen(s),
ii) when expressed in a cell of the plant, the Lr67 polypeptide is not as active at transporting glucose across a membrane of the cell as a polypeptide which comprises the amino acid sequence of SEQ ID NO: 4, and
iii) when expressed in a cell of the plant, the Lr67 polypeptide is active as a sugar transporter.

3. The transgenic plant of claim 2 , wherein the one or more biotrophic fungal pathogen(s) is selected from the group consisting of Blumeria graminis f. sp. tritici, Fusarium graminearum, Bipolaris sorokiniana, Erysiphe graminis f. sp. tritici, Puccinia graminis f. sp. tritici, Puccinia striiformis, Puccinia hordei and Puccinia recondita f. sp. tritici.

4. The transgenic plant of claim 1 , wherein the Lr67 polypeptide comprises an amino acid sequence which is at least 97% identical to the amino acid sequence of SEQ ID NO: 1.

5. The transgenic plant of claim 1 which has enhanced resistance to one or more biotrophic fungal pathogen(s) when compared to an isogenic plant lacking the exogenous polynucleotide.

6. The transgenic plant of claim 1 which has enhanced resistance to one or more biotrophic fungal pathogen(s) at the seedling stage of growth.

7. The transgenic plant of claim 1 which comprises one or more further exogenous polynucleotides encoding a plant pathogen resistance polypeptide other than an Lr67 polypeptide.

8. The transgenic plant of claim 1 which is a cereal plant.

9. The transgenic plant of claim 8 which is a wheat plant.

10. The transgenic plant of claim 1 which is homozygous for the exogenous polynucleotide(s).

11. A seed of the plant of claim 1 , wherein the seed comprises the polynucleotide encoding the Lr67 polypeptide.

12. A product produced from the plant of claim 1 or seed thereof, wherein the product is a food product or beverage product which comprises the exogenous polynucleotide.

13. Flour or wholemeal comprising a transgene comprising an exogenous polynucleotide encoding an Lr67 polypeptide which comprises an amino acid sequence which is at least 95% identical to the amino acid sequence of SEQ ID NO: 1, wherein the Lr67 polypeptide does not comprise a glycine at a position corresponding to amino acid number 144 of SEQ ID NO: 1.

14. A method of producing a plant part, the method comprising:
a) growing the plant of claim 1 to produce a plant part, and
b) harvesting the plant part.

15. A method of producing flour, wholemeal or starch obtained from seed, the method comprising:
a) obtaining seed of claim 11 , and
b) extracting the flour, wholemeal or starch.

16. A method of preparing the food product of claim 12 , the method comprising mixing seed, or flour, wholemeal or starch from the seed, with another food ingredient.

17. A chimeric vector comprising a polynucleotide which encodes an Lr67 polypeptide which comprises an amino acid sequence which is at least 95% identical to the amino acid sequence of SEQ ID NO: 1, wherein the Lr67 polypeptide does not comprise a glycine at a position corresponding to amino acid number 144 of SEQ ID NO: 1, and wherein the polynucleotide is operably linked to a promoter.

18. The chimeric vector of claim 17 , wherein the chimeric vector provides one or more or all of:
i) when expressed in a plant, the Lr67 polypeptide confers upon the plant resistance to one or more biotrophic fungal pathogen(s),
ii) when expressed in a cell, the Lr67 polypeptide is not as active at transporting glucose across a membrane of the cell as a polypeptide which comprises amino acids having a sequence as provided in SEQ ID NO:4, and
iii) when expressed in a cell, the Lr67 polypeptide is active as a sugar transporter.

19. A method of producing a Lr67 polypeptide, the method comprising:
a) cultivating the transgenic plant of claim 1 to produce a Lr67 polypeptide, and
b) isolating the polypeptide from the plant.

20. A method of producing a transgenic plant, the method comprising the steps of:
i) introducing the vector of claim 17 into a cell of a plant, and
ii) regenerating a transgenic plant from the cell.

21. A method of producing a plant which has integrated into its genome a transgene comprising a polynucleotide encoding an Lr67 polypeptide, the method comprising the steps of:
i) crossing two parental plants, wherein at least one parental plant is the plant of claim 1 ,
ii) screening one or more progeny plants from the cross for the presence or absence of the polynucleotide, and
iii) selecting a progeny plant which comprises the polynucleotide, thereby producing the plant.

22. A process for determining whether a polypeptide confers resistance or susceptibility to one or more biotrophic fungal pathogen(s), comprising:
i) obtaining a polynucleotide operably linked to a promoter, the polynucleotide encoding the polypeptide, wherein the polypeptide comprises an amino acid sequence which is at least 95% identical to the amino acid sequence of SEQ ID NO: 1, wherein the polypeptide does not comprise a glycine at a position corresponding to amino acid number 144 of SEQ ID NO: 1,
ii) introducing the polynucleotide into a plant,
iii) determining whether the level of resistance or susceptibility to the one or more biotrophic fungal pathogen(s) is increased or decreased relative to an isogenic plant lacking the polynucleotide, and
iv) optionally, if the level of resistance or susceptibility is increased, selecting a polynucleotide encoding the polypeptide which when expressed confers resistance or susceptibility to the one or more biotrophic fungal pathogen(s).

23. The transgenic plant of claim 1 , wherein the Lr67 polypeptide comprises SEQ ID NO: 1.

24. The flour or wholemeal of claim 13 , wherein the Lr67 polypeptide comprises SEQ ID NO: 1.

25. The chimeric vector of claim 17 , wherein the Lr67 polypeptide comprises SEQ ID NO: 1.

26. The process of claim 22 , wherein the Lr67 polypeptide comprises SEQ ID NO: 1.

27. The method of claim 20 , further comprising producing one or more progeny plants from the regenerated transgenic plant, or harvesting seed from the regenerated transgenic plant or the one or more progeny plants.

Assignees

• Commw Scient Ind Res Org
• Grains Res & Dev Corp

Inventors

• Lagudah Evans
• Moore John Wallace

Classifications

• C12Q2600/158

  Expression markers · CPC title

• C12Q2600/13

  Plant traits · CPC title

• G01N33/5035

  on sub-cellular localization · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• A01N65/08

  Magnoliopsida [dicotyledons] · CPC title