High-flux ultra-sensitive detection dot array enhancement chip

Patent metadata

Field	Value
Publication number	US-12024463-B2
Application number	US-202017066549-A
Country	US
Kind code	B2
Filing date	Oct 9, 2020
Priority date	Jan 31, 2019
Publication date	Jul 2, 2024
Grant date	Jul 2, 2024

Abstract

The disclosure discloses a high-flux and ultra-sensitive detection dot array enhancement chip, and belongs to the field of food safety detection. In the disclosure, single-layer Au nano-particles are chemically bonded onto a hydrophilic substrate, an Au nano-material is naturally deposited in holes of the chip under an electrostatic adsorption action, and a regular dot array is formed. Au particles distributed in the holes are separated with a particle surfactant (CTAB) to form plasma gaps so as to enhance the self-assemble of Au nano-particles distributed on hot-spots for a long range effect, thereby improving the sensing signal in detection efficiency and sensitivity of the chip.

First claim

What is claimed is:

1. A method of use of a dot array chip for detection of a signal,
wherein the dot array chip has a dot array in a form of assembly of Au nano-particles, and particle assemble spots present a plasma enhancement effect in a form of signal enhancement micro-regional spots;
wherein the method comprises placing a to-be-detected sample onto the dot array chip to detect a fluorescence intensity, wherein the dot array chip is a Au nano-particle dot array connected with a nucleotide sequence capable of capturing to-be-detected mRNA; wherein the Au nano-particle dot array is in a form of assembly of Au nano-particles, and particle assemble spots present a plasma enhancement effect in a form of signal enhancement micro-regional spots.

Assignees

• Univ Jiangnan

Inventors

• Pi Fuwei
• Liu Ling
• Li Ying
• Zhang Yuanyuan
• Yin Ziye
• Cheng Yuliang
• Lin Lin
• Sun Xiulan

Classifications

• C03C17/00

  Surface treatment of glass, not in the form of fibres or filaments, by coating (optical coatings of optical elements G02B1/10) · CPC title

• G01N21/65

  Raman scattering · CPC title

• G01N21/6428

  Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53) · CPC title

• C23C14/58

  After-treatment · CPC title

• C23C14/35

  by application of a magnetic field, e.g. magnetron sputtering {(C23C14/3457 takes precedence)} · CPC title