Ciliated protozoan with the effect of promoting plant seed germination and seedling growth

Patent metadata

Field	Value
Publication number	US-11999667-B2
Application number	US-202318330402-A
Country	US
Kind code	B2
Filing date	Jun 7, 2023
Priority date	Jun 9, 2022
Publication date	Jun 4, 2024
Grant date	Jun 4, 2024

Abstract

A method of using a strain of ciliated protozoan for promoting effect on germination and growth of plant seeds is provided. The ciliated protozoan is a Colpoda species JH2-5-4, with preservation number of China Center for Type Culture Collection (“CCTCC”) NO. C202229. A method of screening and selecting a highly efficient and active strain suitable for use as a microbial fertilizer in plant cultivation is provided. The selected ciliated protozoan strain shortens the germination time of plant seeds, promotes plant growth, and improves seedling survival rate.

First claim

What is claimed is:

1. A method for promoting the germination of Arabidopsis thaliana seeds using ciliated protozoa, comprising the steps of:
a) synchronizing the Arabidopsis thaliana seeds by dark incubation at 4ºC for 7 days;
b) disinfecting the synchronized Arabidopsis thaliana seeds in 75% ethanol for 10 minutes and suspending the plant seeds in 0.1% agarose;
c) mixing soil and distilled water, and then subjecting the soil and distilled water to high-pressure steam sterilization to remove preexisting soil microorganisms producing hydrated soil;
d) cutting gauze and placing the gauze at the bottom of each pot of a plurality of pots, wherein the plurality of pots consists of 20 pots;
e) aliquoting the hydrated soil into each pot of the plurality of pots;
f) placing nine seeds in each pot of the plurality of pots;
g) treating the hydrated soil in each pot of the plurality of pots with an Escherichia coli suspension;
h) treating each pot of the plurality of pots with approximately 20,000 ciliated protozoa;
i) labeling each pot, and placing each pot of the plurality of pots onto a tray of a plurality of trays;
j) placing each tray of the plurality of trays in a different culture chamber with a temperature of 25° C., a 16/8 hour (light/dark) cycle, humidity of 65%, and light intensity of 4950 lux; and
k) photographing and watering any Arabidopsis thaliana plants,
wherein the ciliated protozoa are a Colpoda species with a preservation number of China Center for Type Culture Collection No. C202229.

Assignees

• Ocean Univ China

Inventors

• Long Hongan
• Jiang Yurou
• Li Haichao
• Pan Jiao

Classifications

• C05F11/08 Primary

  Organic fertilisers containing added bacterial cultures, mycelia or the like · CPC title

• A01G7/06

  Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants · CPC title

• C12N1/105 Primary

  Protozoal isolates · CPC title

• C12R2001/90

  Protozoa {; Processes using protozoa} · CPC title

• C05F11/00

  Other organic fertilisers · CPC title