Process of recovering oil

Patent metadata

Field	Value
Publication number	US-11965143-B2
Application number	US-202318166975-A
Country	US
Kind code	B2
Filing date	Feb 9, 2023
Priority date	Jun 24, 2013
Publication date	Apr 23, 2024
Grant date	Apr 23, 2024

Abstract

The present invention relates to processes of recovering oil after liquefaction and/or from thin stillage and/or syrup/evaporated centrate from a fermentation product production process by adding a thermostable protease to the whole stillage, thin stillage and/or syrup.

First claim

The invention claimed is:

1. A process of recovering oil, comprising:
(a) liquefying a starch-containing material into dextrins with a bacterial alpha-amylase at a temperature above the initial gelatinization temperature, wherein the starch-containing material comprises corn;
(b) saccharifying the dextrins using a glucoamylase to form a sugar;
(c) fermenting the sugar in a fermentation medium into a fermentation product using a fermenting organism, wherein the fermentation product is fuel ethanol and the fermenting organism is yeast;
(d) recovering the fermentation product to form a whole stillage;
(e) separating the whole stillage into thin stillage and wet cake;
(e′) optionally concentrating the thin stillage into syrup; and
(f) recovering oil from the thin stillage and/or optionally the syrup, wherein a thermostable protease derived from Thermobifida is present or added during step (a).

2. The process of claim 1 , wherein the oil is recovered during and/or after step (a).

3. The process of claim 2 , wherein oil recovery is increased higher than control.

4. The process of claim 1 , wherein the oil is recovered from the thin stillage.

5. The process of claim 4 , wherein oil recovery is increased higher than control.

6. The process of claim 1 , wherein the oil is optionally recovered from the syrup.

7. The process of claim 6 , wherein oil recovery is increased higher than control.

8. The process of claim 1 , wherein liquefying step (a) is performed at a temperature in the range of 80° C-90° C.

9. The process of claim 1 , wherein liquefying step (a) is performed at a pH in the range of 4-7.

10. The process of claim 1 , wherein the alpha-amylase is derived from Bacillus.

11. The process of claim 1 , wherein saccharifying step (b) and fermenting step (c) are performed simultaneously as a simultaneous saccharification and fermentation (SSF).

12. The process of claim 11 , wherein the SSF is performed at a temperature in the range of 25° C-40° C.

13. The process of claim 1 , wherein the saccharification is performed at a pH in the range of 4-5.

14. The process of claim 1 , wherein the starch-containing material further comprises a cereal.

15. The process of claim 1 , wherein the starch-containing material further comprises a starch-containing material selected from the group consisting of wheat, barley, cassava, sorghum, rye, potato, beans, milo, peas, rice, sago, sweet potatoes, tapioca, or any combination thereof.

Assignees

• Novozymes As

Inventors

• Kreel Nathaniel
• Jump Joseph

Classifications

• C11B13/00 Primary

  Recovery of fats, fatty oils or fatty acids from waste materials · CPC title

• C11B3/003

  by enzymes or microorganisms, living or dead · CPC title

• C12N9/2417

  from microbiological source · CPC title

• C12N9/2428

  Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase · CPC title

• C12N9/50

  Proteinases {, e.g. Endopeptidases (3.4.21-3.4.25)} · CPC title