Processes for recovering oil from ethanol production processes

Patent metadata

Field	Value
Publication number	US-10844318-B2
Application number	US-201816121027-A
Country	US
Kind code	B2
Filing date	Sep 4, 2018
Priority date	Jun 24, 2013
Publication date	Nov 24, 2020
Grant date	Nov 24, 2020

Abstract

The present invention relates to processes for recovering/extracting oil from fermentation product production processes based on starch-containing material, wherein an alpha-amylase, a high dosage of protease, and optionally a glucoamylase, are present and/or added in liquefaction. The invention also relates to processes for producing fermentation products and to enzyme compositions suitable for use in processes of the invention.

First claim

The invention claimed is:

1. A process of recovering oil from an ethanol production process comprising the steps of:
a) liquefying starch-containing material at a temperature above the initial gelatinization temperature using:
an alpha-amylase; and
at least 2.5 micro gram protease per gram dry solids (DS), wherein the protease is the one shown in SEQ ID NO: 13 or a protease having at least 90% sequence identity thereto;

b) saccharifying using a glucoamylase;
c) fermenting using a fermenting organism;
d) recovering the fermentation product to form whole stillage;
e) separating the whole stillage into thin stillage and wet cake;
f) optionally concentrating the thin stillage into syrup;

wherein oil is recovered from the:
liquefied starch-containing material after step a); and/or
downstream from fermentation step c).

2. The process of claim 1 , wherein oil is recovered during, after, or both during and after liquefying the starch-containing material; from the whole stillage; from the thin stillage; or from the syrup.

3. The process of claim 1 , wherein 2.5-100 micro gram protease per gram DS are present or added in liquefaction step a).

4. The process of claim 1 , wherein no nitrogen-compound is added in during liquefaction step a), saccharification step b), fermentation step c), or simultaneous saccharification and fermentation (SSF).

5. The process of claim 1 , wherein the starch-containing material is corn.

6. The process of claim 1 , wherein the protease is one having at least 95% sequence identity to SEQ ID NO: 13.

7. The process of claim 1 , wherein the protease has at least 97% sequence identity to SEQ ID NO: 13.

8. The process of claim 1 , wherein the alpha-amylase is a Bacillus amylase.

9. The process of claim 1 , wherein the alpha-amylase is a Bacillus stearothermophilus alpha-amylase.

10. The process of claim 1 , wherein the alpha-amylase is a Bacillus stearothermophilus alpha-amylase having a double deletion of positions I181+G182 or R179+G180 using SEQ ID NO: 1 for numbering.

11. The process of claim 1 , wherein the alpha-amylase has the amino acid sequence of SEQ ID NO: 1 or is a variant thereof having at least 85% sequence identity thereto.

12. The process of claim 1 , wherein the alpha-amylase has at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 1.

13. The process of claim 1 , wherein the alpha amylase has at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 1.

14. The process of claim 1 , further comprising using a glucoamylase in liquefying step (a).

15. The process of claim 1 , further comprising using a glucoamylase in liquefying step (a), wherein the glucoamylase is a Penicillum glucoamylase.

16. The process of claim 1 , further comprising using a glucoamylase in liquefying step (a), wherein the glucoamylase has the amino acid sequence of SEQ ID NO: 14 or is a variant thereof having at least 85% sequence identity thereto.

17. The process of claim 16 , wherein the glucoamylase has at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 14.

18. The process of claim 16 , wherein the glucoamylase has at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 14.

19. The process of claim 1 , further comprising using a pullulanase in liquefying step (a).

Assignees

• Novozymes As

Inventors

• Clark Suzanne
• Matthews John
• Jump Joseph
• Kreel Nathaniel

Classifications

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title

• C12N9/52 Primary

  derived from bacteria {or Archaea} · CPC title

• C12N9/58

  derived from fungi · CPC title

• C12N9/2417

  from microbiological source · CPC title

• C12Y302/01001

  Alpha-amylase (3.2.1.1) · CPC title