Process of recovering oil

Patent metadata

Field	Value
Publication number	US-10920172-B2
Application number	US-201916449703-A
Country	US
Kind code	B2
Filing date	Jun 24, 2019
Priority date	Jun 24, 2013
Publication date	Feb 16, 2021
Grant date	Feb 16, 2021

Abstract

The present invention relates to processes of recovering oil after liquefaction and/or from thin stillage and/or syrup/evaporated centrate from a fermentation product production process by adding a thermostable protease to the whole stillage, thin stillage and/or syrup.

First claim

The invention claimed is:

1. A process of recovering oil, comprising:
(a) converting a starch-containing material into dextrins by liquefaction with a bacterial alpha-amylase;
(b) saccharifying the dextrins using a glucoamylase to form a sugar;
(c) fermenting the sugar in a fermentation medium into a fermentation product using a fermenting organism;
(d) recovering the fermentation product to form a whole stillage;
(e) separating the whole stillage into thin stillage and wet cake;
(e′) optionally concentrating the thin stillage into syrup; and
(f) recovering oil from the thin stillage and/or optionally the syrup, wherein a protease having at least 80% sequence identity to SEQ ID NO: 10 is present and/or added during step (a).

2. The process of claim 1 , wherein the protease has at least 90% sequence identity to SEQ ID NO: 10.

3. The process of claim 1 , wherein the protease has at least 95% sequence identity to SEQ ID NO: 10.

4. The process of claim 1 , wherein the protease has at least 97% sequence identity to SEQ ID NO: 10.

5. The process of claim 1 , wherein the oil is recovered during and/or after step (a).

6. The process of claim 1 , wherein the oil is recovered from the thin stillage.

7. The process of claim 1 , wherein the oil is optionally recovered from the syrup.

8. The process of claim 1 , wherein the fermentation product is ethanol.

9. The process of claim 1 , wherein the bacterial alpha-amylase is the Bacillus stearothermophilus alpha-amylase having the sequence of SEQ ID NO: 1 herein, which is truncated to have from 485-495 amino acids, or one having at least 80% sequence identity thereto.

10. The process of claim 1 , wherein oil recovery is increased higher than a control without protease.

11. The process of claim 1 , wherein oil recovery is increased by at least 25%.

12. The process of claim 11 , wherein oil recovery is increased by at least 100%.

13. The process of claim 1 , wherein the starch-containing material is corn.

14. The process of claim 1 , wherein the temperature is between 80-90° C.

15. The process of claim 1 , wherein saccharifying step (b) and fermenting step (c) are carried out simultaneously.

16. The process of claim 1 , wherein the bacterial alpha-amylase is derived from the genus Bacillus.

17. The process of claim 1 , wherein the protease is derived from a strain of Thermobifida.

18. The process of claim 1 , wherein the fermentation product is fuel ethanol.

19. The process of claim 18 , wherein oil recovery is increased higher than a control without protease.

20. The process of claim 19 , wherein the oil is recovered during and/or after step (a).

21. The process of claim 20 , wherein oil recovery is increased by at least 100%.

22. The process of claim 19 , wherein the oil is recovered from the thin stillage.

23. The process of claim 18 , wherein the starch-containing material is corn.

24. The process of claim 23 , wherein oil recovery is increased higher than a control without protease.

25. The process of claim 24 , wherein the oil is recovered during and/or after step (a).

26. The process of claim 25 , wherein oil recovery is increased by at least 100%.

27. The process of claim 24 , wherein the oil is recovered from the thin stillage.

28. The process of claim 23 wherein saccharifying step (b) and fermenting step (c) are carried out simultaneously.

29. The process of claim 28 , wherein oil recovery is increased higher than a control without protease.

30. The process of claim 29 , wherein the oil is recovered during and/or after step (a).

31. The process of claim 30 , wherein oil recovery is increased by at least 100%.

32. The process of claim 29 , wherein the oil is recovered from the thin stillage.

33. The process of claim 28 , wherein the temperature is between 80-90° C.

34. The process of claim 33 , wherein oil recovery is increased higher than a control without protease.

35. The process of claim 34 , wherein the oil is recovered during and/or after step (a).

36. The process of claim 34 , wherein oil recovery is increased by at least 100%.

37. The process of claim 34 , wherein the oil is recovered from the thin stillage.

38. The process of claim 18 , 23 , 28 , and 33 , wherein the bacterial alpha-amylase is derived from the genus Bacillus.

39. The process of claim 38 , wherein oil recovery is increased higher than a control without protease.

40. The process of claim 39 , wherein the oil is recovered during and/or after step (a).

41. The process of claim 40 , wherein oil recovery is increased by at least 100%.

42. The process of claim 39 , wherein the oil is recovered from the thin stillage.

43. The process of claim 39 , 40 , 41 , and 42 , wherein the protease is derived from a strain of Thermobifida.

44. The process of claim 18 , 23 , 28 , and 33 , wherein the protease is derived from a strain of Thermobifida.

45. The process of claim 44 , wherein oil recovery is increased higher than a control without protease.

46. The process of claim 45 , wherein the oil is recovered during and/or after step (a).

47. The process of claim 46 , wherein oil recovery is increased by at least 100%.

48. The process of claim 45 , wherein the oil is recovered from the thin stillage.

49. The process of claim 45 , 46 , 47 and 48 , wherein the bacterial alpha-amylase is derived from the genus Bacillus.

Assignees

• Novozymes As

Inventors

• Kreel Nathaniel
• Jump Joseph

Classifications

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title

• B01D3/002

  by continuous methods · CPC title

• C12Y302/01003

  Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase · CPC title

• C12N9/58

  derived from fungi · CPC title

• C12P7/10

  substrate containing cellulosic material · CPC title