Surface stabilization of biosensors

Patent metadata

Field	Value
Publication number	US-11879156-B2
Application number	US-202217818869-A
Country	US
Kind code	B2
Filing date	Aug 10, 2022
Priority date	Mar 5, 2015
Publication date	Jan 23, 2024
Grant date	Jan 23, 2024

Abstract

A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

First claim

What is claimed:

1. A semiconductor apparatus comprising:
an array of semiconductor sensors formed on a semiconductor substrate;
an array of wells formed over the array of semiconductor sensors, wherein each semiconductor sensor includes a field effect transistor having a gate structure; and
a removable saccharide coating disposed over the array of wells and the gate structure.

2. The semiconductor apparatus of claim 1 , further comprising a flow cell lid disposed over the semiconductor substrate and the array of wells and defining a flow cell between the substrate and the flow cell lid.

3. The semiconductor apparatus of claim 1 , wherein the gate structure is a floating gate structure.

4. The semiconductor apparatus of claim 3 , wherein the floating gate includes a sensor plate and a sensor layer disposed over the sensor plate.

5. The semiconductor apparatus of claim 4 , wherein the sensor layer includes a ceramic layer.

6. The semiconductor apparatus of claim 1 , wherein the field effect transistor is a chemical field effect transistor.

7. The semiconductor apparatus of claim 6 , wherein the chemical field effect transistor is an ion-sensitive field effect transistor.

8. The semiconductor apparatus of claim 1 , wherein the removable saccharide coating includes a monosaccharide, a disaccharide, a polysaccharide, a derivation thereof, or a combination thereof.

9. The semiconductor apparatus of claim 8 , wherein the monosaccharide includes glucose, fructose, galactose, or a combination thereof.

10. The semiconductor apparatus of claim 8 , wherein the disaccharide includes sucrose, trehalose, maltose, or lactose.

11. The semiconductor apparatus of claim 10 , wherein the disaccharide includes sucrose.

12. The semiconductor apparatus of claim 10 , wherein the disaccharide includes trehalose.

13. The semiconductor apparatus of claim 1 , wherein the removable saccharide coating includes a surfactant.

14. The semiconductor apparatus of claim 13 , wherein the surfactant is selected from a non-ionic fluorosurfactant, an alkyl polysaccharide surfactant, an alkyl sulfonate surfactant or a zwitterionic surfactant.

15. The semiconductor apparatus of claim 14 , wherein the alkyl polysaccharide surfactant comprises dioctyl sodium sulfosuccinate.

16. The semiconductor apparatus of claim 14 , wherein the zwitterionic surfactant comprises anzergent 3-12.

17. The semiconductor apparatus of claim 1 , wherein the removable saccharide coating is a conformal coating.

18. The semiconductor apparatus of claim 1 , wherein the removable saccharide coating is a protective coating.

19. The semiconductor apparatus of claim 18 , wherein the protective coating is configured to be removed before the semiconductor apparatus is used.

Assignees

• Life Technologies Corp

Inventors

• Waggoner Phil
• Ball James A
• Hinz Wolfgang
• Minto Michael L
• Parker Scott
• Cox David M
• Mastroianni Alexander
• Gray Jeremy
• Glazer Marc
• GORRELL Kimberly

Classifications

• C12Q1/6869 Primary

  Methods for sequencing · CPC title

• G01N27/4145 Primary

  specially adapted for biomolecules, e.g. gate electrode with immobilised receptors · CPC title

• C12Q2527/119

  pH · CPC title

• C12Q2527/125

  Specific component of sample, medium or buffer · CPC title

• C12Q2565/607

  being a sensor, e.g. electrode · CPC title