Surface Stabilization of Biosensors

Patent metadata

Field	Value
Publication number	US-2016258011-A1
Application number	US-201615061273-A
Country	US
Kind code	A1
Filing date	Mar 4, 2016
Priority date	Mar 5, 2015
Publication date	Sep 8, 2016
Grant date	—

Abstract

A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

First claim

What is claimed is:

1 . A sensor apparatus comprising:
a substrate; a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure; and a saccharide coating formed over the surface electrode structure.

2 . The sensor apparatus of claim 1 , further comprising a well structure defined over the semiconductor device and defining a well exposing the surface electrode structure, the saccharide coating formed at least partially within the well.

3 . The sensor apparatus of claim 2 , further comprising a bead disposed in the well, the saccharide coating formed over the bead.

4 . The sensor apparatus of claim 3 , wherein the bead is conjugated to a nucleic acid.

5 . The sensor apparatus of claim 3 , wherein the bead is a hydrogel bead.

6 . The sensor apparatus of claim 3 , wherein the bead comprises a polymer selected from the group consisting of agarose; polyethylene glycol; polyoxybutylene;
diethylacrylamide; polyoxyethylene; polyacrylamide; polyoxypropylene; N,N-polydimethylacrylamide; poly(N-isopropylacrylamide); polyvinylpyrrolidone; poly-N-hydroxyacrylamide; and any combination thereof.

7 . The sensor apparatus of claim 6 , wherein the polymer is polyacrylamide.

8 . The sensor apparatus of claim 1 , further comprising a flow cell lid disposed over the substrate and defining a flow cell between the substrate and the flow cell lid, the surface electrode structure accessible through the flow cell.

9 . The sensor apparatus of claim 1 , wherein the semiconductor device includes a field effect transistor having a gate electrode structure, the surface electrode structure forming at least part of the gate electrode structure.

10 . The sensor apparatus of claim 1 , wherein the surface electrode structure includes a conductive structure and a sensor layer disposed over the conductive structure.

11 . The sensor apparatus of claim 10 , wherein the sensor layer includes a ceramic layer.

12 . The sensor apparatus of claim 1 , wherein the surface electrode structure includes a floating gate structure.

13 . The sensor apparatus of claim 1 , wherein the saccharide coating includes a monosaccharide, a disaccharide, a polysaccharide, a derivation thereof, or a combination thereof.

14 . The sensor apparatus of claim 13 , wherein the monosaccharide includes glucose, fructose, galactose, or a combination thereof.

15 . The sensor apparatus of claim 13 , wherein the disaccharide includes sucrose, trehalose, maltose, or lactose.

16 . The sensor apparatus of claim 15 , wherein the disaccharide includes sucrose.

17 . The sensor apparatus of claim 15 , wherein the disaccharide includes trehalose.

18 . A sensor apparatus comprising:
a semiconductor substrate; semiconductor devices formed on the semiconductor substrate; a well structure formed over the semiconductor devices and defining a well, wherein the semiconductor devices include a field effect transistor having a gate structure responsive to a condition within the well; and a saccharide coating disposed within the well and over the gate structure.

19 . The sensor apparatus of claim 18 , further comprising a flow cell lid disposed over the semiconductor substrate and the well structure and defining a flow cell between the substrate and the flow cell lid, the well exposed to the flow cell.

20 . The sensor apparatus of claim 18 , further comprising a bead is disposed in the well, the saccharide coating disposed over the bead.

Assignees

• Life Technologies Corp

Inventors

• Waggoner Phil
• Ball James A
• Hinz Wolfgang
• Minto Michael L
• Parker Scott
• Cox David M
• Mastroianni Alexander
• Gray Jeremy
• Glazer Marc
• GORRELL Kimberly

Classifications

• C12Q1/6869 Primary

  Methods for sequencing · CPC title

• G01N27/4145 Primary

  specially adapted for biomolecules, e.g. gate electrode with immobilised receptors · CPC title

• C12Q2527/125

  Specific component of sample, medium or buffer · CPC title

• C12Q2527/119

  pH · CPC title

• C12Q2565/607

  being a sensor, e.g. electrode · CPC title