Fabrication methods for bio-compatible devices
US-9685689-B1 · Jun 20, 2017 · US
Surface stabilization of biosensors
US10619201B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10619201-B2 |
| Application number | US-201816182384-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 6, 2018 |
| Priority date | Mar 5, 2015 |
| Publication date | Apr 14, 2020 |
| Grant date | Apr 14, 2020 |
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- Title
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Abstract
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A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of sequencing, the method comprising: flowing a wash solution over a sensor apparatus, the sensor apparatus comprising: a substrate; a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure; a well structure defining a well over the surface electrode structure, a bead disposed in the well; and a saccharide coating formed over the surface electrode structure and the bead; the wash solution removing the saccharide coating; applying nucleic acid targets to the sensor apparatus; applying the sensor apparatus to a sequencing system; and sequencing the nucleic acid targets using the sequencing system. 2. The method of claim 1 , wherein the saccharide coating includes a monosaccharide, a disaccharide, a polysaccharide, a derivation thereof, or a combination thereof. 3. The method of claim 2 , wherein the monosaccharide includes glucose, fructose, galactose, or a combination thereof. 4. The method of claim 2 , wherein the disaccharide includes sucrose, trehalose, maltose, or lactose. 5. The method of claim 4 , wherein the disaccharide includes sucrose. 6. The method of claim 2 , wherein the saccharide includes hyaluronan. 7. The method of claim 1 , wherein applying nucleic acid targets to the sensor apparatus includes contacting the nucleic acid targets with the bead. 8. The method of claim 7 , wherein the bead includes an oligonucleotide complementary to a portion of the nucleic acid target, the method further comprising amplifying the nucleic acid target to form copies of the nucleic acid target on the bead. 9. The method of claim 7 , wherein the bead is a hydrogel bead. 10. The method of claim 7 , wherein the bead comprises a polymer selected from the group consisting of agarose; polyethylene glycol; polyoxybutylene; diethylacrylamide; polyoxyethylene; polyacrylamide; polyoxypropylene; N,N-polydimethylacrylamide; poly(N-isopropylacrylamide); polyvinylpyrrolidone; poly-N-hydroxyacrylamide; and any combination thereof.
Assignees
Inventors
Classifications
- G01N27/4145Primary
specially adapted for biomolecules, e.g. gate electrode with immobilised receptors · CPC title
- C12Q1/6869Primary
Methods for sequencing · CPC title
being a sensor, e.g. electrode · CPC title
Specific component of sample, medium or buffer · CPC title
pH · CPC title
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- What does patent US10619201B2 cover?
- A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.
- Who is the assignee on this patent?
- Life Technologies Corp
- What technology area does this patent fall under?
- Primary CPC classification G01N27/4145. Mapped technology areas include Physics.
- When was this patent published?
- Publication date Tue Apr 14 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 3 related publications on this page (citations in our corpus or others sharing the same primary CPC).