Processes for producing ethanol

Patent metadata

Field	Value
Publication number	US-11840718-B2
Application number	US-202218052080-A
Country	US
Kind code	B2
Filing date	Nov 2, 2022
Priority date	Dec 22, 2010
Publication date	Dec 12, 2023
Grant date	Dec 12, 2023

Abstract

The present invention relates to processes for producing fermentation products from starch-containing material, wherein a thermostable alpha-amylase and optionally a thermostable protease are present and/or added during liquefaction. The invention also relates to a composition suitable for use in a process of the invention.

First claim

The invention claimed is:

1. A process for producing a fermentation product from starch-containing material comprising:
i) liquefying the starch-containing material at a temperature above the initial gelatinization temperature using:
an alpha-amylase; and
a protease having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 13;

ii) saccharifying the liquefied material obtained in step i) using a glucoamylase; and
iii) fermenting the saccharified material obtained in step (ii) using a fermenting organism.

2. The process of claim 1 , wherein the alpha-amylase is a variant of a Bacillus alpha-amylase.

3. The process of claim 1 , wherein the alpha-amylase is a variant of a Bacillus stearothermophilus alpha-amylase.

4. The process of claim 1 , wherein the alpha-amylase has at least 80%, but less than 100% identity to the mature part of the polypeptide of SEQ ID NO: 1.

5. The process of claim 4 , wherein the alpha-amylase is truncated.

6. The process of claim 1 , wherein the alpha-amylase is a truncated Bacillus stearothermophilus alpha-amylase with the mutations selected from the group consisting of:
I181*+G182*; and
I181*+G182*+N193F; wherein each position corresponds to a position in the amino acid sequence set forth in SEQ ID NO: 1.

7. The process of claim 1 , wherein the alpha-amylase has a T½ (min) at pH 4.5, 85° C., 0.12 mM CaCl 2 ) of at least 10.

8. The process of claim 1 , wherein the alpha-amylase has a T½ (min) at pH 4.5, 85° C., 0.12 mM CaCl2) of at least 15.

9. The process of claim 1 , wherein the alpha-amylase has a T½ (min) at pH 4.5, 85° C., 0.12 mM CaCl2) of at least 20.

10. The process of claim 1 , wherein the alpha-amylase has a T½ (min) at pH 4.5, 85° C., 0.12 mM CaCl2) of at least 25.

11. The process of claim 1 , wherein the alpha-amylase has a T½ (min) at pH 4.5, 85° C., 0.12 mM CaCl2) of at least 30.

12. The process of claim 1 , wherein the alpha-amylase has a T½ (min) at pH 4.5, 85° C., 0.12 mM CaCl2) of at least 40.

13. The process of claim 1 , wherein the alpha-amylase has a T½ (min) at pH 4.5, 85° C., 0.12 mM CaCl2) of at least 50.

14. The process of claim 1 , wherein the alpha-amylase has a T½ (min) at pH 4.5, 85° C., 0.12 mM CaCl2) of at least 60.

15. The process of claim 1 , wherein the alpha-amylase has a T½ (min) at pH 4.5, 85° C., 0.12 mM CaCl2) of at least 70.

16. The process of claim 1 , wherein the protease has at least 96% sequence identity to the amino acid sequence of SEQ ID NO: 13.

17. The process of claim 1 , wherein the protease has as at least 97% sequence identity to the amino acid sequence of SEQ ID NO: 13.

18. The process of claim 1 , wherein the protease has as at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 13.

19. The process of claim 1 , wherein the protease has as at least 99% sequence identity to the amino acid sequence of SEQ ID NO: 13.

20. The process of claim 1 , wherein the protease comprises, consists essentially of, or consists of the amino acid sequence of SEQ ID NO: 13.

Assignees

• Novozymes As

Inventors

• Deinhammer Randy
• Clark Suzanne
• Quiros Mauricio
• Matthews John
• Hjulmand Anne Glud
• Soong Chee-Leong
• Matsui Tomoko
• Takagi Shinobu

Classifications

• C12P7/06 Primary

  Ethanol, i.e. non-beverage · CPC title

• C12Y302/01001

  Alpha-amylase (3.2.1.1) · CPC title

• C12Y302/01003

  Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase · CPC title

• C12Y304/24

  Metalloendopeptidases (3.4.24) · CPC title

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title