System for preparing solutions for chromatography

Patent metadata

Field	Value
Publication number	US-11801456-B2
Application number	US-202117156749-A
Country	US
Kind code	B2
Filing date	Jan 25, 2021
Priority date	Dec 12, 2014
Publication date	Oct 31, 2023
Grant date	Oct 31, 2023

Abstract

A system for preparing solutions for chromatography application is disclosed. The system comprises a T-joint for preparing a buffer solution by mixing at least one first solution and a second solution. The T-joint receives the second solution from a solution supply unit connected to the T-joint. Further one or more low pressure pumps supply the one or more first solutions into the T-joint. The high pressure pump collects the buffer solution and delivers it to a chromatography apparatus.

First claim

I claim:

1. A system for performing inline blending of liquids, the system comprising:
a T-joint for preparing a buffer solution by mixing at least one first solution and a second solution, wherein the T-joint receives the second solution from a solution supply unit connected to the T-joint, and the T-joint is connected to the solution supply unit using at least one direct connection tube, and wherein the T-joint is connected through a check valve to a further solution supply unit for supplying the at least one first solution;
at least one low pressure pump for supplying the at least one first solution into the T-joint;
a high pressure pump for collecting and delivering the buffer solution to a chromatography apparatus; and
a control system configured to control operation of the at least one low pressure pump for supplying the at least one first solution, responsive to sensing of at least one sensed characteristic of the buffer solution between the T-joint and the high pressure pump;
the system being characterized in that said T-joint includes a configuration for mixing the at least one first solution and the second solution; and
wherein the system does not comprise a pump for supplying the second solution to the T-joint.

2. The system of claim 1 , wherein the configuration for mixing the at least one first solution and the second solution is a magnetic stirrer.

3. The system of claim 1 , wherein the at least one first solution comprises a salt solution, an acid, a base, or a combination thereof.

4. The system of claim 1 , wherein the control system is configured to control the flow rate of the at least one first solution supplied by the at least one low pressure pump, and wherein the flow rate of the at least one first solution and a flow rate of the high pressure pump determines a concentration of the buffer solution.

5. The system of claim 1 further comprising at least one sensor configured to measure the at least one characteristic of the buffer solution placed between the T-joint and the high pressure pump.

6. The system of claim 5 , wherein a sensor of the at least one sensor is configured to measure a conductivity associated with the buffer solution.

7. The system of claim 6 , wherein a sensor of the at least one sensor is configured to measure a pH level associated with the buffer solution.

8. A chromatography system comprising:
at least one chromatography column;
a T-joint for preparing a buffer solution by mixing at least one first solution and a second solution, wherein the T-joint receives the second solution from a solution supply unit connected to the T-joint, wherein the T-joint is connected to the solution supply unit using at least one direct connection tube, and wherein the T-joint is connected through a check valve to a further solution supply unit for supplying the at least one first solution;
at least one low pressure pump for supplying the at least one first solution into the T-joint;
a high pressure pump for collecting and delivering the buffer solution to the at least one chromatography column; and
a control system configured to control operation of the at least one low pressure pump for supplying the at least one first solution, responsive to sensing of at least one sensed characteristic of the buffer solution between the T-joint and the high pressure pump;
the chromatography system being characterized in that said T-joint includes a configuration for mixing the at least one first solution and the second solution; and
wherein the chromatography system does not comprise a pump for supplying the second solution to the T-joint.

9. The system of claim 8 , wherein the configuration for mixing the at least one first solution and the second solution is a magnetic stirrer.

10. The chromatography system of claim 8 , wherein the at least one first solution comprises a salt solution, an acid, a base, or a combination thereof.

11. The chromatography system of claim 8 , wherein the control system is configured to control the flow rate of the at least one first solution supplied by the at least one low pressure pump, and wherein the flow rate of the at least one first solution and a flow rate of the high pressure pump determines concentration of the buffer solution.

12. The chromatography system of claim 8 further comprising at least one sensor configured to measure the at least one characteristic of the buffer solution placed between the T-joint and the high pressure pump.

13. The chromatography system of claim 12 , wherein a sensor of the at least one sensor is configured to measure a conductivity associated with the buffer solution.

14. The chromatography system of claim 12 , wherein a sensor of the at least one sensor is configured to measure a pH level associated with the buffer solution.

15. A system for performing inline blending or mixing of liquids in a chromatography apparatus, the system comprising:
a T-joint for preparing a buffer solution by blending or mixing at least two initial solutions;
a liquid supply unit for each of the at least two initial solutions, wherein the liquid supply units of one or more of the at least two initial solutions communicate with at least one respective pump and the liquid supply unit of one or more of the at least two initial solutions not communicating with a pump, wherein the T-joint is connected to each of the liquid supply units not communicating with a pump by at least one direct connection tube, and wherein the T-joint is connected through a check valve to each of the liquid supply units communicating with a pump;
a liquid egress for removing the buffer solution from the T-joint, the liquid egress including a further pump operable at least to draw in solution from each of the liquid supply units not communicating with a pump and each of the liquid supply which has supply units which communicate with a pump, and operable to deliver the buffer solution to a chromatography apparatus; and
a control system configured to control operation of the at least one pump for supplying the at least one respective initial solution, responsive to sensing of at least one sensed characteristic of the buffer solution between the T-joint and the further pump;
the system being characterized in that said T-joint includes a configuration for mixing the at least two initial solutions.

16. The system of claim 15 , wherein the configuration for mixing the at least two initial solutions is a magnetic stirrer.

Assignees

• Cytiva Sweden Ab

Inventors

• Olovsson Bjorn Markus

Classifications

• F04B23/04

  Combinations of two or more pumps · CPC title

• B01D15/166 Primary

  Fluid composition conditioning, e.g. gradient · CPC title

• B01D15/22

  relating to the construction of the column · CPC title

• B01F23/49

  Mixing systems, i.e. flow charts or diagrams · CPC title

• B01F25/31

  in conduits or tubes through which the main component flows · CPC title