System for preparing solutions for chromatography

Patent metadata

Field	Value
Publication number	US-10926191-B2
Application number	US-201515533405-A
Country	US
Kind code	B2
Filing date	Dec 10, 2015
Priority date	Dec 12, 2014
Publication date	Feb 23, 2021
Grant date	Feb 23, 2021

Abstract

A system for preparing solutions for chromatography application is disclosed. The system comprises a T-joint for preparing a buffer solution by mixing at least one first solution and a second solution. The T-joint receives the second solution from a solution supply unit connected to the T-joint. Further one or more low pressure pumps supply the one or more first solutions into the T-joint. The high pressure pump collects the buffer solution and delivers it to a chromatography apparatus.

First claim

I claim:

1. A system for performing inline blending of liquids, the system comprising:
at least one first liquid solution disposed in a first solution supply unit;
a second solution supply unit;
a T-joint for preparing a buffer solution by mixing the at least one first liquid solution and a second liquid solution, wherein the T-joint receives the second liquid solution from the second solution supply unit connected to the T-joint, and the T-joint is connected to the second solution supply unit (i) through a check valve, and (ii) through at least one direct connection tube;
at least one low pressure pump for supplying the at least one first liquid solution into the T-joint from the first solution supply unit;
a high pressure pump for collecting the buffer solution and delivering to a chromatography apparatus; and
at least one sensor configured to measure one or more characteristics of the buffer solution received at the high pressure pump, wherein the at least one sensor is arranged between the at least one low pressure pump and the high pressure pump the at least one sensor being located closer to the at least one low pressure pump than to the high pressure pump such that operation of the at least one sensor is not affected by high pressure generated by the high pressure pump.

2. The system of claim 1 , wherein the at least one first liquid solution comprises at least one of an acid, a base and a salt solution.

3. The system of claim 1 , further comprising a control system configured to: control operation of the at least one low pressure pump for supplying the at least one first liquid solution.

4. The system of claim 3 , wherein the control system is configured to control a flow rate of the at least one first liquid solution supplied by the at least one low pressure pump, the flow rate of the at least one first liquid solution and a flow rate of the high pressure pump determining concentration of the buffer solution.

5. The system of claim 1 , wherein a sensor of the at least one sensor is configured to measure a conductivity associated with the buffer solution.

6. The system of claim 1 , wherein a sensor of the at least one sensor is configured to measure a pH level associated with the buffer solution.

7. A chromatography system comprising:
at least one first liquid solution disposed in a first solution supply unit;
a second solution supply unit;
at least one chromatography column;
a T-joint for preparing a buffer solution by mixing the at least one first liquid solution and a second liquid solution, wherein the T-joint receives the second liquid solution from the second solution supply unit connected to the T-joint, and the T-joint is connected to the second solution supply unit (i) through a check valve, and (ii) through at least one direct connection tube;
at least one low pressure pump for supplying the at least one first liquid solution into the T-joint from the first solution supply unit;
a high pressure pump for collecting and delivering the buffer solution to the at least one chromatography column; and
at least one sensor configured to measure one or more characteristics of the buffer solution received at the high pressure pump, wherein the at least one sensor is arranged between the at least one low pressure pump and the high pressure pump, the at least one sensor being located closer to the at least one low pressure pump than to the high pressure pump such that operation of the at least one sensor is not affected by high pressure generated by the high pressure pump.

8. The chromatography system of claim 7 , wherein the at least one first liquid solution comprises at least one of an acid, a base and a salt solution.

9. The chromatography system of claim 7 , further comprising a control system configured to: control operation of the at least one low pressure pump for supplying the at least one first liquid solution and control the functioning of the T-joint to prepare the buffer solution.

10. The chromatography system of claim 9 , wherein the control system is configured to control a flow rate of the at least one first liquid solution supplied by the at least one low pressure pump, the flow rate of the at least one first liquid solution and a flow rate of the high pressure pump determining concentration of the buffer solution.

11. The chromatography system of claim 7 , wherein a sensor of the at least one sensor is configured to measure a conductivity associated with the buffer solution.

12. The chromatography system of claim 7 , wherein a sensor of the at least one sensor is configured to measure a pH level associated with the buffer solution.

13. A system for performing inline blending or mixing of liquids in chromatography apparatus, the system comprising:
a T-joint for preparing a buffer solution by blending or mixing at least two initial liquid solutions;
a liquid supply for each of the initial liquid solutions, for supplying the respective initial liquid solutions to the T-joint, one or more of the liquid supplies having a pump and one or more of the remaining liquid supplies having no pump, wherein the T-joint is connected to one or more of the liquid supplies (i) through a check valve, and (ii) through at least one direct connection tube; and
a liquid egress for removing the buffer solution from the T-joint, the liquid egress including a further pump operable at least to draw in solution from the or each liquid supply which has no pump, and operable to deliver the buffer solution to a chromatography apparatus; and
at least one sensor configured to measure one or more characteristics of the buffer solution received at the further pump, wherein the at least one sensor is arranged between the T-joint and the further pump, the at least one sensor being located closer to the one or more pumps of the liquid supplies than to the further pump such that operation of the at least one sensor is not affected by high pressure generated by the further pump.

14. A system as claimed in claim 13 , further comprising a control configured to control a respective flow rate of the at least one first liquid solution supplied by the one or more pumps of the liquid supplies, the respective flow rates of the at least one first liquid solution supplied by the one or more pumps of the liquid supplies and a flow rate of the further pump determining concentration of the buffer solution.

Assignees

• Cytiva Sweden Ab

Inventors

• Olovsson Bjorn Markus

Classifications

• F04B23/04

  Combinations of two or more pumps · CPC title

• B01F35/2211

  Amount of delivered fluid during a period · CPC title

• B01F35/2133

  Electrical conductivity or dielectric constant of the mixture · CPC title

• B01F35/2132

  Concentration, pH, pOH, p(ION) or oxygen-demand (B01F35/2133 takes precedence) · CPC title

• B01F25/31

  in conduits or tubes through which the main component flows · CPC title