Nanopore device for reversible ion and molecule sensing or migration

Patent metadata

Field	Value
Publication number	US-11709148-B2
Application number	US-202117557549-A
Country	US
Kind code	B2
Filing date	Dec 21, 2021
Priority date	Mar 4, 2011
Publication date	Jul 25, 2023
Grant date	Jul 25, 2023

Abstract

Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.

First claim

What is claimed is:

1. A nanopipette for measuring pH of a sample, the nanopipette comprising:
(a) a capillary portion defining an interior bore of the nanopipette leading to a nanopore;
(b) the interior bore adapted for containing therein an electrode and an interior solution communicating with an exterior solution through said nanopore; and
(c) a coating on an interior surface of the nanopore, the coating comprising:
(i) a polyelectrolyte layer bound directly to the interior surface; and
(ii) a chitosan molecule linked to the polyelectrolyte layer.

2. The nanopipette of claim 1 , wherein the polyelectrolyte layer is a polyacrylic layer.

3. The nanopipette of claim 1 , wherein the nanopipette is a quartz nanopipette.

4. The nanopipette of claim 1 , wherein the nanopipette is a glass nanopipette.

5. The nanopipette of claim 1 , wherein the nanopore opening has an outer diameter of less than 1 mm.

6. The nanopipette of claim 1 , wherein the nanopore opening has inner diameter of less than 200 nm.

7. The nanopipette of claim 1 , wherein the nanopore opening has inner diameter of 40-60 nm.

8. The nanopipette of claim 1 , wherein the coating comprises alternating layers of polyacrylic and chitosan.

9. A nanopipette apparatus for measuring pH of a sample, the apparatus comprising:
(a) a nanopipette having an interior bore and a nanopore opening into the sample;
(b) an electrode within the interior bore, arranged to contact an interior solution, and a reference electrode positionable in the sample;
(c) a coating on an inner surface of the nanopore;
(d) the coating comprising:
(i) a polyelectrolyte layer bound directly to the interior surface; and
(ii) a chitosan molecule linked to the polyelectrolyte layer; and

(e) a voltage control circuit for generating a voltage between electrodes and measuring ionic current through the sample, the nanopore, and the interior solution.

10. The nanopipette apparatus of claim 9 , wherein the nanopipette is quartz.

11. The nanopipette apparatus of claim 9 , wherein the polyelectrolyte layer is a polyacrylic layer.

12. A method for measuring pH of a sample, the method comprising:
contacting the sample with the nanopipette apparatus of claim 9 ; and
using the voltage control circuit for measuring ionic current, wherein the current is correlated to pH of the sample.

13. The method of claim 12 , wherein the nanopipette is quartz.

14. The method of claim 12 , wherein the polyelectrolyte layer is a polyacrylic layer.

15. The method of claim 12 , wherein the sample comprises a single cell and the method measures pH of the cell.

16. The nanopipette of claim 9 , wherein the nanopipette is a glass nanopipette.

17. The nanopipette of claim 9 , wherein the nanopore opening has an outer diameter of less than 1 mm.

18. The nanopipette of claim 9 , wherein the nanopore opening has inner diameter of less than 200 nm.

19. The nanopipette of claim 9 , wherein the nanopore opening has inner diameter of 40-60 nm.

20. The nanopipette of claim 9 , wherein the coating comprises alternating layers of polyacrylic and chitosan.

Assignees

• Univ California

Inventors

• Pourmand Nader
• Vilozny Boaz
• Actis Paolo
• Seger R Adam

Classifications

• G01N27/44791 Primary

  Microapparatus (sample containers with integrated microfluidic structures B01L3/5027) · CPC title

• B01L3/021 Primary

  Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids · CPC title

• B01L3/50273

  characterised by the means or forces applied to move the fluids · CPC title

• B82Y35/00

  Methods or apparatus for measurement or analysis of nanostructures · CPC title

• G01N27/3277

  being a redox reaction, e.g. detection by cyclic voltammetry (voltammetry per se G01N27/42, G01N27/48) · CPC title