Nanopore device for reversible ion and molecule sensing or migration

Patent metadata

Field	Value
Publication number	US-10345260-B2
Application number	US-201514641064-A
Country	US
Kind code	B2
Filing date	Mar 6, 2015
Priority date	Mar 4, 2011
Publication date	Jul 9, 2019
Grant date	Jul 9, 2019

Abstract

Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.

First claim

What is claimed is:

1. A method for creating an ionic compound from two different ions in solution, comprising:
(a) providing at least one first ion species having a charge in a first solution inside of a nanopipette, said nanopipette having a nanopore between an interior of the nanopipette and an exterior solution;
(b) providing a second ion species in the exterior solution,

wherein the first solution and the exterior solution have the same polarity; and
(c) applying, to the first ion species having a charge in the first solution inside of the nanopipette, a voltage across the nanopore of opposite charge from the charge on the first ion species, said voltage sufficient to cause migration of said first ion species to the nanopore to react with said second ion species to form an ionic compound,
wherein the nanopipette has a capillary portion defining an interior bore of the nanopipette, wherein the interior bore is elongated and tapers to a tip with the nanopore positioned at the tip of the nanopipette, wherein the nanopore has an inner diameter in the range of 37 nm-82 nm and the outer diameter of the nanopore is less than 1 μm,
wherein the first solution is different from the exterior solution and prior to step (c), the first and second ion species are isolated from each other in the first and exterior solutions, respectively.

2. The method of claim 1 further comprising the step of measuring ionic current through the nanopore and detecting a change in current indicative of formation of the ionic compound.

3. The method of claim 1 wherein said change in current indicative of formation of the ionic compound is an oscillation.

4. The method of claim 1 wherein the ionic compound is insoluble.

5. The method of claim 1 wherein either the first ion species or the second ion species is a metal cation reacting with an anion to form the ionic compound.

6. The method of claim 5 wherein the metal cation is a transition metal cation.

7. The method of claim 5 wherein the metal cation is selected from the group consisting of: Mg2+, Ca2+, Mn2+, Zn2+, Cu2+, Fe2+, Fe3+, Cr3+, Cr6+, Cd2+, Mo2+, Co3+, Co2+, Hg2+, Ni2+, Al3+, Al2+, Ar3+, Ar3−, and Pb2+.

8. The method of claim 1 wherein the first ion species or the second ion species is an anion selected from the group consisting of: phosphate, chloride, sulfate, monophosphate, pyrophosphate, metaphosphate, tripolyphosphate, tetrametaphosphate, and orthophosphate.

9. The method of claim 1 wherein the first ion species or the second ion species is an anion, wherein the anion is an organic carboxylic acid anion selected from the group consisting of: gluconate, tartrate, fumarate, maleate, malonate, malate, lactate, citrate, EDTA, citraconate, citramalate, stearate, oleate, laurate, octoate, ascorbate, picolinate, and orotate.

10. The method of claim 1 wherein either the first ion species or the second ion species is a protein.

11. The method of claim 1 further comprising the step of reversing said voltage after a precipitate has formed.

12. The method of claim 1 further comprising the step of detecting the formation of an ionic compound created.

13. The method of claim 1 , wherein the first solution and the exterior solution are aqueous solutions.

14. The method of claim 1 , wherein the nanopore has an inner diameter is in the range of 40 nm-60 nm.

15. The method of claim 1 , wherein the first ion species is negatively charged and the second ion species is positively charged and wherein the voltage is a negative voltage.

16. A method for creating an ionic compound from two different ions in solution, comprising:
(a) providing at least one first ion species having a charge in a first solution inside of a nanopipette, with a nanopore between an interior of the nanopipette and an exterior solution,
wherein the nanopore has an inner diameter in the range of 37 nm-82 nm and the outer diameter of the nanopore is less than 1 μm;
(b) providing a second ion species in the exterior solution; and
(c) applying to the first ion species, having a charge in the first solution inside of the nanopipette, a voltage across the nanopore of opposite charge, said voltage sufficient to cause migration of the first ion species to the nanopore to react with the second ion species to form an ionic compound,
wherein the first solution is different from the exterior solution and prior to step (c), the first and second ion species are isolated from each other in the first and exterior solutions, respectively,
wherein the nanopipette has a capillary portion defining an interior bore of the nanopipette leading to the nanopore and a coating on an interior surface of the nanopore, the coating comprising:
(i) a polyelectrolyte layer bound directly to the interior surface; and
(ii) a binding molecule, linked to the polyelectrolyte layer, specific for binding an analyte which is selected from the group consisting of an ion or a small molecule having a molecular weight of less than 200 atomic mass units.

17. The method of claim 16 , further comprising the step of measuring ionic current through the nanopore and detecting a change in current indicative of formation of the ionic compound.

18. The method of claim 16 , wherein the binding molecule is a boronic acid or boronic esters.

19. The method of claim 16 , wherein the polyelectrolyte layer is a polycation layer.

Assignees

• Univ California

Inventors

• Pourmand Nader
• Vilozny Boaz
• Actis Paolo
• Seger R Adam

Classifications

• G01N33/48721

  Investigating individual macromolecules, e.g. by translocation through nanopores (Coulter counters in general G01N15/12; fabrication methods for nanoscale apertures B81B1/00; sequencing of nucleic acids C12Q1/68) · CPC title

• G01N27/44791 Primary

  Microapparatus (sample containers with integrated microfluidic structures B01L3/5027) · CPC title

• B82Y35/00

  Methods or apparatus for measurement or analysis of nanostructures · CPC title

• B01L3/021 Primary

  Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids · CPC title

• B01L3/50273

  characterised by the means or forces applied to move the fluids · CPC title