Solid state sequencing devices comprising two dimensional layer materials

Patent metadata

Field	Value
Publication number	US-11656197-B2
Application number	US-201716479257-A
Country	US
Kind code	B2
Filing date	Nov 22, 2017
Priority date	Jan 19, 2017
Publication date	May 23, 2023
Grant date	May 23, 2023

Abstract

A sequencing device is disclosed. The sequence device includes an array of conducting electrode pairs, each pair of electrodes comprising a source and a drain electrode arrangement separated by a nanogap, the electrode array deposited and patterned on a dielectric substrate; at least one transition metal dichalcogenide (TMD) layer disposed on each pair of electrodes, wherein the TMD layer connects each source and drain electrode within each pair, and bridges each nanogap of each pair of electrodes; and a dielectric masking layer disposed on the TMD layer and comprising at least one opening that defines an exposed TMD region, wherein the at least one opening is sized so as to allow a single biomolecule to fit therein and to attach on to the exposed TMD region. In embodiments of the disclosure, the TMD layer be a defective TMD layer.

First claim

What is claimed is:

1. A device to characterize a biomolecule, comprising:
an array of conducting electrode pairs, each pair of electrodes comprising a source and a drain electrode arrangement separated by a nanogap, the electrode array deposited and patterned on a dielectric substrate;
at least one transition metal dichalcogenide (TMD) layer disposed on each pair of electrodes, wherein the TMD layer connects each source and drain electrode within each pair, and bridges each nanogap of each pair of electrodes;
a dielectric masking layer disposed on the TMD layer and comprising an aperture in the dielectric masking layer to expose a portion of the TMD region, wherein the aperture is sized to allow one and only one biomolecule to attach to the exposed TMD region through the aperture; and
a single enzyme molecule connected to the exposed TMD region via an attachment consisting of a functional group pair such that only one enzyme molecule is present within each of the apertures.

2. The device of claim 1 , wherein the enzyme molecule comprises a polymerase enzyme.

3. The device of claim 1 , further comprising a microfluidic system in fluid combination with the sequencing device.

4. The device of claim 1 , wherein the at least one TMD layer comprises MoS 2 , WS 2 , TiS 2 , ZrS 2 , HfS 2 , VS 2 , NbS 2 , TaS 2 , TcS 2 , ReS 2 , CoS 2 , RhS 2 , IrS 2 , NiS 2 , PdS 2 , PtS 2 , or any of their modifications or combinations, including modified stoichiometry of sulfur contents having MX (2−x) or MX (2+x) , wherein x is in the range of 0-1.0.

5. The device of claim 4 , wherein the sulfur stoichiometry is altered to provide vacancy defects, interstitial defects, and aggregated defects so as to increase surface energy and enhance adhesion of the enzyme molecule to the exposed TMD region.

6. The device of claim 1 , wherein the at least one TMD layer comprises MoSe 2 , WSe 2 , TiSe 2 , ZrTe 2 , HfTe 2 , VTe 2 , NbTe 2 , TaTe 2 , TcTe 2 , ReTe 2 , CoTe 2 , RhTe 2 , IrTe 2 , NiTe 2 , PdTe 2 , PtTe 2 , or any of their modifications or combinations, including modified stoichiometry of selenium contents having MX (2−x) or MX (2+x) , wherein x is in the range of 0-1.0.

7. The device of claim 6 , wherein the selenium stoichiometry is intentionally altered to provide vacancy defects, interstitial defects, and aggregated defects in order to increase surface energy of the TMD layer and enhance adhesion of the enzyme molecule to the exposed TMD region.

8. The device of claim 1 , wherein the at least one TMD layer comprises MoTe 2 , WTe 2 , TiTe 2 , ZrTe 2 , HfTe 2 , VTe 2 , NbTe 2 , TaTe 2 , TcTe 2 , ReTe 2 , CoTe 2 , RhTe 2 , IrTe 2 , NiTe 2 , PdTe 2 , PtTe 2 , or any of their modifications or combinations, including modified stoichiometry of tellurium contents having MX (2−x) or MX (2+x) , wherein x is in the range of 0-1.0.

9. The device of claim 8 , wherein the tellurium stoichiometry is intentionally altered to provide vacancy defects, interstitial defects, and aggregated defects in order to increase surface energy of the TMD layer and enhance adhesion of the enzyme molecule to the exposed TMD region.

10. The device of claim 1 , wherein the TMD layer comprises (Mo x W y Co z )S 2 ) or (Hf x W y Co z )Te 2 .

11. The device of claim 1 , wherein the array of conducting electrode pairs comprise at least one of Au, Pt, Ag, Pd, Rh, or their alloys.

12. The device of claim 1 , wherein the nanogap is about 2 nm to about 20 nm in length.

13. The device of claim 1 , wherein the TMD layer comprises a defective TMD layer.

14. The device of claim 13 , wherein the defective TMD layer comprises a linear nano-ribbon parallel array, a patterned shape nano-ribbon array, strained lattice defects, vacancies, interstitial defects, dislocation defects, foreign atom implanted defects, or nanoporous defects.

15. The device of claim 13 , wherein the defective TMD layer comprises strained lattice defects, vacancies, interstitial defects, dislocation defects or foreign atom implanted defects with a defect density of at least about 10 5 /cm 2 .

16. The device of claim 13 , wherein the defective TMD layer comprises nanoporous defects having an equivalent diameter of at least 2 nm with a defect density of at least 10 3 /cm 2 .

17. The device of claim 13 , wherein the defective TMD layer has a bandgap opened to a value of at least 0.2 eV.

18. The device of claim 1 , wherein the functional group pair is a streptavidin biotin pair, an antigen-antibody interaction, bifunctional ligands using mercaptocarbonic acids [HS-(CH 2 )n-COOH, n=1-15], peptide functional groups, a thiol-alkyne pair, a COOH-NH 2 functional group pair, a thiol-maleimide azide pair, a silanization linkage using mercaptosilane compounds, or a NHS (N-hydoxysuccinimide) ester-amine pair.

19. A device to characterize a biomolecule, comprising:
an array of conducting electrode pairs, each pair of electrodes comprising a source and a drain electrode arrangement separated by a nanogap, the electrode array deposited and patterned on a dielectric substrate;
a transition metal dichalcogenide (TMD) layer disposed on each pair of electrodes, wherein the TMD layer connects each source and drain electrode within each pair, and bridges each nanogap of each pair of electrodes;
a dielectric masking layer disposed on the TMD layer and comprising an aperture that defines an exposed TMD region; and
a single enzyme molecule connected to the exposed TMD region via an attachment consisting of a functional group pair such that only one enzyme molecule is connected through the aperture;
wherein the aperture is formed through the TMD layer to receive one and only one biomolecule.

20. The device of claim 19 , wherein the TMD comprises a defective MoS 2 .

21. The device of claim 19 , wherein the aperture is lithographically formed.

22. The device of claim 19 , wherein the aperture is formed to have a diameter of about 30 nm or less.

Assignees

• Roswell Biotechnologies Inc
• Roswell Me Inc

Inventors

• Choi Chulmin
• Jin Sungho
• Merriman Barry L
• Mola Paul
• Geiser Tim

Classifications

• G01N33/48721

  Investigating individual macromolecules, e.g. by translocation through nanopores (Coulter counters in general G01N15/12; fabrication methods for nanoscale apertures B81B1/00; sequencing of nucleic acids C12Q1/68) · CPC title

• B82Y5/00

  Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery · CPC title

• G01N27/4145 Primary

  specially adapted for biomolecules, e.g. gate electrode with immobilised receptors · CPC title

• C12Q1/68

  involving nucleic acids · CPC title

• C12Q1/527

  involving lyase · CPC title