Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells

Patent metadata

Field	Value
Publication number	US-11653635-B2
Application number	US-201916708007-A
Country	US
Kind code	B2
Filing date	Dec 9, 2019
Priority date	Jun 6, 2012
Publication date	May 23, 2023
Grant date	May 23, 2023

Abstract

The invention relates to methods of increasing the genetic progress of a line, breed or herd of swine through the use of sex-selected sperm cells in artificial insemination techniques. The invention also encompasses methods of artificially inseminating a swine via deep intrauterine catheter or via a laparoscopic procedure, which allow the use of reduced doses of sex-selected sperm cells.

First claim

What is claimed is:

1. A method of increasing the genetic progress of a line or breed of swine within a genetic nucleus comprising the steps of:
providing a boar from a genetic nucleus and a sow from a genetic nucleus, wherein said boar and said sow are from a line or breed within said genetic nucleus and wherein said line or breed of said boar and said sow are the same line or breed;
collecting a semen sample from said boar;
sorting said semen sample into at least two subpopulations of sperm cells, wherein at least 80% of a first subpopulation bears X-chromosomes or Y-chromosomes;
administering a hormone or hormone analog to said sow;
detecting ovulation in said sow;
inseminating said sow with 50×10 6 or less sperm cells from said first subpopulation by deep intrauterine catheter or by laparoscopy;
producing offspring from said sow;
calculating one or more estimated breeding values for said offspring; and
selecting one or more of said offspring as a parent in the line or breed in the genetic nucleus based on the one or more determined estimated breeding values.

2. The method of claim 1 , wherein the one or more estimated breeding values are calculated by best linear unbiased prediction.

3. The method of claim 1 , wherein ovulation is detected by ultrasound.

4. The method of claim 1 , wherein the hormone or hormone analog is selected from the group consisting of: progesterone; a progestin; and a gonadotropin.

5. The method of claim 1 , wherein said sow is inseminated with 1×10 6 or less sperm cells from said first subpopulation.

6. The method of claim 1 , wherein the deep intrauterine catheter is 1 to 3 meters in length.

7. The method of claim 1 , wherein said sow is inseminated laparoscopically in one or more of said sow's utero-tubal junctions.

8. The method of claim 1 , wherein said sow is inseminated laparoscopically in one or more of said sow's isthmus.

Assignees

• Inguran Llc

Inventors

• Moreno Juan
• Bevier Gregg
• Dobrinsky John

Classifications

• A01K2227/108

  Swine · CPC title

• G16B20/40

  Population genetics; Linkage disequilibrium · CPC title

• A61B1/015

  Control of fluid supply or evacuation · CPC title

• A01K67/02 Primary

  Breeding vertebrates · CPC title

• A61M31/002

  Devices for releasing a drug at a continuous and controlled rate for a prolonged period of time (artificial gland structures or devices A61F2/022; intra-uterine contraceptive devices A61F6/14; tampons for introducing into the vagina A61F13/20, A61L15/00; suppositories or bougies for intra-vaginal or intra-uterine application A61K9/02; physical forms of medicinal preparations for sustained or differential drug release A61K9/20, A61K9/50) · CPC title