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Sperm diluent solution and method for artificial insemination using same
US9439414B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9439414-B2 |
| Application number | US-201013378560-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 17, 2010 |
| Priority date | Jun 17, 2009 |
| Publication date | Sep 13, 2016 |
| Grant date | Sep 13, 2016 |
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- Title
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Abstract
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A sperm diluent of the present invention contains a chelating agent such as EDTA and/or, EGTA, which forms a complex with a calcium ion, in a base diluent. Further, the sperm diluent contains an immunosuppressive factor such as a steroid hormone and/or, a cytokine, which suppresses migration of leukocytes. By diluting frozen sperm with this sperm diluent followed by performing artificial insemination, death of the sperm before fertilization and phagocytosis of the sperm and embryos by leukocytes in the uterus can be suppressed, allowing enhancement of the conception rate and the implantation rate.
First claim
Opening claim text (preview).
The invention claimed is: 1. A pig sperm diluent comprising chelating agents which form a complex with a calcium ion, and an immunosuppressive factor which suppresses migration of leukocytes; the chelating agents being 3 to 9 mM of ethylene glycol tetraacetic acid and 3 to 9 mM of ethylenediaminetetraacetic acid, the immunosuppressive factor being cortisol. 2. The pig sperm diluent according to claim 1 , which is used for diluting frozen sperm prepared by removal of seminal plasma and freezing. 3. A method for artificial insemination of a pig, comprising: preparing frozen sperm by removing seminal plasma from semen collected from a pig and, upon melting, diluting the sperm with the pig sperm diluent according to claim 1 , to prepare artificial semen; and injecting said artificial semen into a uterus of a female pig to carry out artificial insemination. 4. The pig sperm diluent according to claim 1 , wherein the cortisol is in an amount of 100 ng to 10,000 ng.
Assignees
Inventors
Classifications
- A01N1/125Primary
Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators · CPC title
Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells (vaccines or medicinal preparations containing antigens or antibodies A61K39/00) · CPC title
Calcium; Ca chelators; Calcitonin · CPC title
- A01N1/0221Primary
Human Necessities · mapped topic
Conditioning of cells for in vitro fecondation or nuclear transfer · CPC title
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- What does patent US9439414B2 cover?
- A sperm diluent of the present invention contains a chelating agent such as EDTA and/or, EGTA, which forms a complex with a calcium ion, in a base diluent. Further, the sperm diluent contains an immunosuppressive factor such as a steroid hormone and/or, a cytokine, which suppresses migration of leukocytes. By diluting frozen sperm with this sperm diluent followed by performing artificial insemi…
- Who is the assignee on this patent?
- Shimada Masayuki, Okazaki Tetsuji, Univ Hiroshima
- What technology area does this patent fall under?
- Primary CPC classification A01N1/125. Mapped technology areas include Human Necessities.
- When was this patent published?
- Publication date Tue Sep 13 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).