Collagen-binding agent compositions and methods of using the same

Patent metadata

Field	Value
Publication number	US-11624060-B2
Application number	US-201816485290-A
Country	US
Kind code	B2
Filing date	Feb 9, 2018
Priority date	Feb 10, 2017
Publication date	Apr 11, 2023
Grant date	Apr 11, 2023

Abstract

The invention generally relates to collagen-binding agent compositions and methods of using the same. More specifically, the invention relates in part to new collagen-binding agent compositions and methods that may be used to treat damaged collagen within tissues or used to specifically target therapeutics to tissues containing undamaged or damaged collagen.

First claim

We claim:

1. A collagen-binding agent comprising a first collagen-binding domain and a second collagen-binding domain linked by a domain linker and a therapeutic agent linked to at least one of the collagen-binding domains by a therapeutic agent linker,
wherein the collagen-binding agent lacks collagenase activity;
wherein the therapeutic agent is fibroblast growth factor (FGF);
wherein the two collagen-binding domains bind to collagen with a K D of between 100 μM and 0.1 nM;
wherein the first collagen-binding domain is selected from the group consisting of the polypeptides of SEQ ID NOs: 1-14, and the second collagen-binding domain is selected from the group consisting of the polypeptides of SEQ ID NOs: 15-30; and
wherein the C-terminus of the first collagen-binding domain is linked to the N-terminus of the second collagen-binding domain by the domain linker.

2. The collagen-binding agent of claim 1 , wherein the domain linker comprises a polypeptide.

3. The collagen-binding agent of claim 1 , wherein the domain linker comprises a polypeptide selected from the group consisting of the polypeptides of SEQ ID NOs: 48-55 or a polypeptide having at least 80% sequence identity to any one of the polypeptides of SEQ ID NOs: 48-55.

4. The collagen-binding agent of claim 1 , wherein the therapeutic agent linker comprises a polypeptide.

5. The collagen-binding agent of claim 4 , wherein the C-terminus of the therapeutic agent is linked to the N-terminus of the collagen-binding domains by the therapeutic agent linker.

6. A pharmaceutical composition comprising the collagen-binding agent of claim 1 and a pharmaceutical carrier.

7. A method of stimulating bone formation in a subject having a bone condition comprising administering to the subject a therapeutically effective amount of the collagen-binding agent of claim 1 .

8. The method of claim 7 , wherein the subject is a mammal.

9. The method of claim 8 , wherein the mammal is a human.

Assignees

• Univ Arkansas
• The Kitasato Inst

Inventors

• Sakon Joshua
• Roeser Jeffrey
• Bauer Ryan
• Janowska Katarzyna
• Tanaka Keisuke
• Matsushita Osamu
• Uchida Kentaro

Classifications

• C07K14/78

  Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG] · CPC title

• A61Q7/00

  Preparations for affecting hair growth · CPC title

• C07K14/635

  Parathyroid hormone, i.e. parathormone; Parathyroid hormone-related peptides · CPC title

• C07K14/503

  basic FGF [bFGF] · CPC title

• C07K14/71

  for growth factors; for growth regulators · CPC title