Delivery of therapeutic agents by a collagen binding protein

Patent metadata

Field	Value
Publication number	US-9526765-B2
Application number	US-201314378067-A
Country	US
Kind code	B2
Filing date	Feb 11, 2013
Priority date	Feb 9, 2012
Publication date	Dec 27, 2016
Grant date	Dec 27, 2016

Abstract

Methods of delivering therapeutic agents by administering compositions including a bacterial collagen-binding polypeptide segment linked to the therapeutic agent to subjects in need of treatment with the therapeutic agent are provided. Methods of treating hyperparathyroidism, and hair loss using compositions comprising a collagen binding polypeptide and a PTH/PTHrP receptor agonist are provided. In addition, methods of reducing hair regrowth by administering a composition including a collagen binding polypeptide and a PTH/PTHrP receptor antagonist are provided.

First claim

We claim:

1. A method of slowing hair growth comprising administering a composition comprising a bacterial collagen-binding polypeptide segment linked to a PTH/PTHrP receptor antagonist to a subject to slow hair growth relative to the hair growth the subject would experience without treatment, wherein the bacterial collagen-binding polypeptide segment comprises a collagen-binding polypeptide derived from an M9 peptidase selected from the group consisting of, one of SEQ ID NOs: 13-34 or a fragment of at least 8 consecutive amino acids of SEQ ID NOs: 13-34, residues 34-158 of SEQ ID NO: 1, a fragment of at least 8 consecutive amino acids from residues 34-158 of SEQ ID NO: 1, and a peptide that is at least 90% identical to residues 34-158 of SEQ ID NO: 1 or SEQ ID NOs: 13-34, and wherein the PTH/PTHrP receptor antagonist comprises a sequence selected from the group consisting of residues 7-33 of SEQ ID NO: 7, residues 7-14 of SEQ ID NO: 7, a fragment of at least 8 consecutive amino acids from residues 7-34 of SEQ ID NO: 7, and residues ((−1)-33) of PTH.

2. The method of claim 1 , wherein the composition is administered locally.

3. The method of claim 2 , wherein the composition is administered topically.

4. The method of claim 1 , wherein and the N-terminus of the collagen-binding polypeptide segment is linked directly or through a linker polypeptide segment to the C-terminus of the PTH/PTHrP receptor antagonist polypeptide.

5. The method of claim 1 , wherein the collagen-binding polypeptide segment and the PTH/PTHrP receptor antagonist polypeptide are chemically cross-linked to each other or are polypeptide portions of a fusion protein.

6. The method of claim 1 , wherein the subject is human.

7. The method of claim 1 , wherein the bacterial collagen-binding polypeptide segment comprises residues 34-158 of SEQ ID NO: 1 or a peptide that is at 90% identical to residues 34-158 of SEQ ID NO: 1.

8. The method of claim 1 , wherein the PTH/PTHrP receptor antagonist comprises residues 7-33 of SEQ ID NO: 7.

9. The method of claim 1 , wherein the bacterial collagen-binding polypeptide segment linked to the PTH/PTHrP receptor antagonist is a fusion protein comprising SEQ ID NO: 10.

10. The method of claim 4 , wherein the linker polypeptide segment comprises a PKD domain.

11. The method of claim 10 , wherein the PKD domain comprises residues 807-901 of SEQ ID NO: 6.

12. The method of claim 1 , wherein the composition is administered after the subject undergoes a hair removal procedure.

Assignees

• Univ Arkansas
• The Kitasato Inst
• Montefiore Med Center

Inventors

• Ponnapakkam Tulasi
• Philominathan Sagaya Theresa Leena
• Sakon Joshua
• Katikaneni Ranjitha
• Koide Takaki
• Matsushita Osamu
• Gensure Robert C

Classifications

• C12Y304/00

  Hydrolases acting on peptide bonds, i.e. peptidases (3.4) · CPC title

• A61K47/64

  Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent (peptidic linkers A61K47/65) · CPC title

• A61K38/29 Primary

  Parathyroid hormone, i.e. parathormone; Parathyroid hormone-related peptides · CPC title

• A61K38/48

  acting on peptide bonds (3.4) · CPC title

• A61K47/56

  the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule · CPC title