Growth factor anchoring type bone graft material, method for producing growth factor anchoring type bone graft material, kit for producing growth factor anchoring type bone graft material, and method for forming bone

Patent metadata

Field	Value
Publication number	US-9248164-B2
Application number	US-201214117599-A
Country	US
Kind code	B2
Filing date	Mar 26, 2012
Priority date	May 13, 2011
Publication date	Feb 2, 2016
Grant date	Feb 2, 2016

Abstract

Provided is a growth factor anchoring type bone graft material, wherein a bone graft substrate exposing at least a collagen fiber is bound to a collagen-binding-site-containing growth factor which contains a growth factor receptor agonist peptide and a collagen-binding peptide. The same can be produced by mixing a bone graft substrate and a collagen-binding-site-containing growth factor which contains a growth factor receptor agonist peptide and a collagen-binding peptide, and is also superior in osteogenic ability.

First claim

The invention claimed is:

1. A growth factor anchoring type bone graft material, wherein a bone graft substrate exposing at least a collagen fiber is bound to a collagen-binding-site-containing growth factor which comprises a growth factor receptor agonist peptide and a collagen-binding peptide,
wherein the bone graft substrate is a high-density collagen material in a sheet form with a collagen fiber density of 100 to 800 mg/cm 3 , and the collagen-binding-site-containing growth factor is formed by ligating a basic fibroblast growth factor and the collagen-binding peptide through a polycystic kidney disease I domain of a collagenase.

2. A kit for production of a growth factor anchoring type bone graft material wherein a bone graft substrate exposing at least a collagen fiber is bound to a collagen-binding-site-containing growth factor which comprises a growth factor receptor agonist peptide and a collagen-binding peptide,
wherein the bone graft substrate is a high-density collagen material in a sheet form with a collagen fiber density of 100 to 800 mg/cm 3 , and the collagen-binding-site-containing growth factor is formed by ligating a basic fibroblast growth factor and the collagen-binding peptide through a polycystic kidney disease I domain of a collagenase, which kit comprises: a solution comprising a collagen-binding-site-containing growth factor formed by ligating a basic fibroblast growth factor and the collagen-binding peptide through a polycystic kidney disease I domain of a collagenase;
and a bone graft substrate which is said high-density collagen material in a sheet form with the collagen fiber density of 100 to 800 mg/cm 3 .

Assignees

• Uchida Kentaro
• Naruse Koji
• Takaso Masashi
• MIMA Takehiko
• Matsushita Osamu
• Haraguchi Takashi
• Nishi Nozomu
• School Juridical Person Kitasato Inst
• Univ Kagawa Nat Univ Corp

Inventors

• Uchida Kentaro
• Naruse Koji
• Takaso Masashi
• MIMA Takehiko
• Matsushita Osamu
• Haraguchi Takashi
• Nishi Nozomu

Classifications

• C07K14/33

  from Clostridium (G) · CPC title

• A61L27/3608

  Bone, e.g. demineralised bone matrix [DBM], bone powder · CPC title

• C07K14/485

  Epidermal growth factor [EGF], i.e. urogastrone · CPC title

• A61L27/54 Primary

  Biologically active materials, e.g. therapeutic substances {(A61L27/227 takes precedence)} · CPC title

• A61L2430/02

  for reconstruction of bones; weight-bearing implants · CPC title