Structures for automated, multi-stage processing of nanofluidic chips

Patent metadata

Field	Value
Publication number	US-11548000-B2
Application number	US-201816203171-A
Country	US
Kind code	B2
Filing date	Nov 28, 2018
Priority date	Nov 28, 2018
Publication date	Jan 10, 2023
Grant date	Jan 10, 2023

Abstract

Techniques regarding one or more structures that can facilitate automated, multi-stage processing of one or more nanofluidic chips are provided. For example, one or more embodiments described herein can comprise a system, which can comprise a roller positioned adjacent to a microfluidic card comprising a plurality of fluid reservoirs in fluid communication with a plurality of nanofluidic chips. An arrangement of the plurality of nanofluidic chips on the microfluidic card can defines a processing sequence driven by a translocation of the roller across the microfluidic card.

First claim

What is claimed is:

1. A system, comprising:
a roller adjacent to a microfluidic card and that translocates over a surface of the microfluidic card thereby pressurizing fluid in the microfluidic card, the microfluidic card comprising a plurality of fluid reservoirs in fluid communication with a plurality of nanofluidic chips, wherein the plurality of nanofluidic chips on the microfluidic card are structurally arranged in a defined ordered sequence along a length of the microfluidic card, wherein the defined ordered sequence is an ordered series of stages, wherein the defined ordered sequence facilitates processing of the fluid in a time ordered sequence; and
a controller programmed to control operation of a motor to:
drive the translocation of the roller across the microfluidic card and the processing of fluid driven by the translocation of the roller across the microfluidic card to carry out the defined ordered sequence; and
adjust pressure applied on the microfluidic card by the roller via upshift of the torque on the roller.

2. The system of claim 1 , wherein the roller is configured to translocate across the microfluidic card to exert mechanical force against the plurality of fluid reservoirs and to drive a fluid stored within the plurality of fluid reservoirs through the plurality of nanofluidic chips, and wherein the processing comprises a quantity of the fluid being pressurized through at least one of the plurality of nanofluidic chips, and an output stream of one or more particle size fractions are collected in chambers within the microfluidic card.

3. The system of claim 2 , wherein the defined ordered sequence comprises the ordered series of stages, and wherein a first stage from the ordered series of stages comprises the roller positioned onto a first fluid reservoir from the plurality of fluid reservoirs and the fluid flowing from the first fluid reservoir, through a first nanofluidic chip from the plurality of nanofluidic chips and into a second fluid reservoir from the plurality of fluid reservoirs, wherein an output of a first stage is upstream of the roller and the system is disposed such that the output is fed into the roller and pressurizes to drive the processing of the next, downstream one of the plurality of nanofluidic chips.

4. The system of claim 3 , wherein a second stage from the ordered series of stages comprises the roller positioned onto the second fluid reservoir and the fluid flowing from the second fluid reservoir, through a second nanofluidic chip from the plurality of nanofluidic chips, and into a third fluid reservoir from the plurality of fluid reservoirs.

5. The system of claim 2 , further comprising:
a holder plate upon which the microfluidic card is located; and
the motor that drives the holder plate in a conveyance path towards the roller, wherein the roller is a valve adapted to seal off backflow at a location at which the roller contacts the microfluidic chip.

6. The system of claim 5 , further comprising:
a sensor positioned along the conveyance path that detects a position of the holder plate, wherein the controller is further programmed to drive the holder plate based on the position of the holder plate along the conveyance path.

7. The system of claim 2 , wherein the roller comprises a contact area and a non-contact area positioned over the microfluidic card, and wherein the contact area is disposed to exert the mechanical force against the plurality of fluid reservoirs during the translocation across the microfluidic card, and wherein a circumference of the non-contact region is less than a second circumference of the contact region such that clearance is maintained between the non-contact region and an elastic membrane disposed over the microfluidic card.

8. The system of claim 7 , wherein the microfluidic card further comprises a second reservoir in fluid communication with a nanofluidic chip from the plurality of nanofluidic chips, wherein the second reservoir collects a processed output from the at least one of the plurality of nanofluidic chips, and wherein a clearance between the roller and the second reservoir is maintained by the non-contact area during the translocation across the microfluidic card.

9. An apparatus, comprising:
a plurality of nanofluidic chips embedded within a substrate wherein the plurality of nanofluidic chips on the microfluidic card are structurally arranged in a defined ordered sequence;
an elastomer film disposed over the plurality of nanofluidic chips and the substrate, wherein the elastomer film is selectively bonded to the substrate to pattern regions that are bonded to the substrate and regions that are unbonded to the substrate, wherein the pattern of bonded and unbonded regions of the elastomer film defines a plurality of fluid reservoirs and a plurality of fluidic channels, and wherein the plurality of fluid reservoirs are in fluid communication with the plurality of nanofluidic chips by the plurality of fluidic channels, and wherein the elastic nature of the elastomer film allows one or more of the plurality of fluid reservoirs to swell and protrude up from the substrate; and
a controller programmed to control operation of a motor to:
drive the translocation of a roller across a microfluidic card and the processing of fluid driven by the translocation of the roller across the microfluidic card to carry out the defined ordered sequence; and
cause translocation up and translocation down of the roller to avoid contacting features of the microfluidic card that should not be pressed by the roller during translocation of the roller across the microfluidic card.

10. The apparatus of claim 9 , further comprising:
an input reservoir from the plurality of fluid reservoirs that supplies a fluid to the plurality of nanofluidic chips;
an output reservoir from the plurality of fluid reservoirs that receives an output fluid from the plurality of nanofluidic chips; and
a middle film disposed between the plurality of nanofluidic chips and the elastomer film provides a barrier against evaporation or contamination.

11. The apparatus of claim 9 , further comprising:
wherein the apparatus is configured to apply a force to the plurality of fluid reservoirs and deforms a structure of the plurality of fluid reservoirs to pressure the fluid such that the fluid is transferred from at least one of the plurality of nanofluidic chips to e at least a second of the plurality of nanofluidic chips by an external force applied to the plurality of fluid reservoirs and deform a structure of the plurality of fluid reservoirs to pressure the fluid.

12. The apparatus of claim 9 , further comprising:
an inlet device positioned adjacent to the substrate and in fluid communication with the plurality of fluid reservoirs, wherein the inlet device comprises a clamp that pinches an inlet channel to facilitate loading of a sample fluid from the inlet channel into the plurality of fluid reservoirs without an introduction of air into the plurality of fluid reservoirs.

13. The apparatus of claim 9 , further comprising:
an inlet device positioned in fluid communication with the plurality of fluid reservoirs, wherein the inlet device comprises a plug positioned within a port located on the substrate that is in fluid communication with an inlet channel, and wherein an end of the plug located within the port is tapered, wherein the plug ejects air contained within the port in response to insertion of the plug into the port.

14. The apparatus of claim 13 , wherein the end of the plug further comprises a projection that pierces a sealing membrane on the substrate upon insertion into the port

Assignees

• IBM

Inventors

• Wunsch Benjamin
• Smith Joshua T
• Gifford Stacey
• Kim Sung-Cheol

Classifications

• B01L2200/0642

  Filling fluids into wells by specific techniques · CPC title

• B01L2300/06

  Auxiliary integrated devices, integrated components · CPC title

• B01L2400/08

  Regulating or influencing the flow resistance · CPC title

• B01L3/50273 Primary

  characterised by the means or forces applied to move the fluids · CPC title

• B01L2400/0481

  squeezing of channels or chambers · CPC title