Droplet libraries

Patent metadata

Field	Value
Publication number	US-11534727-B2
Application number	US-201615290378-A
Country	US
Kind code	B2
Filing date	Oct 11, 2016
Priority date	Jul 18, 2008
Publication date	Dec 27, 2022
Grant date	Dec 27, 2022

Abstract

The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.

First claim

What is claimed is:

1. An emulsion library comprising:
a plurality of stable aqueous droplets within an immiscible oil comprising at least one surfactant, wherein each droplet is uniform in size and most of the droplets comprise:
one bead comprising an optical label specific for a target, wherein the bead is linked to a first antibody that binds to the target; and
a second enzyme-linked antibody that binds to the target,

wherein the droplets do not include the target and do not coalesce during storage and further wherein the oil and the surfactant have been selected to prevent the exchanges of contents among the droplets during the storage, wherein the emulsion library includes beads labeled with a plurality of optical labels, wherein each of the plurality of optical labels comprises a dye of increasing concentration from one label to the next.

2. The emulsion library of claim 1 , wherein the surfactant is a block copolymer consisting of one or more perfluorinated polyether (PFPE) blocks and one or more polyethylene glycol (PEG) blocks.

3. The emulsion library of claim 2 , wherein the surfactant is a triblock copolymer consisting of a PEG center block covalently bound to two PFPE blocks by amide linking groups.

4. The emulsion library of claim 1 , wherein the emulsion library is stable for at least 30 days.

5. An emulsion library comprising:
a plurality of stable aqueous droplets within an immiscible oil comprising at least one surfactant, wherein each droplet is uniform in size and most of the droplets comprise:
one bead comprising an optical label specific for a target, wherein the bead is linked to a first antibody that binds to the target; and
a second enzyme-linked antibody that binds to the target,

wherein the droplets do not include the target and do not coalesce during storage and further wherein the oil and the surfactant have been selected to prevent the exchanges of contents among the droplets during the storage, wherein the aqueous droplets have been stored have been stored for a period greater than 90 days under conditions that tend to promote droplet coalescence and wherein the droplets have a stable shelf life and do not coalesce for the period greater than 90 days.

6. The emulsion library of claim 1 , wherein the bead in each droplet comprises a magnetic bead.

7. An emulsion library comprising:
a plurality of stable aqueous droplets within an immiscible oil comprising at least one surfactant, wherein each droplet is uniform in size and most droplets comprise:
exactly one bead comprising an optical label specific for a target, wherein the bead is linked to a first antibody that binds to the target; and
a second antibody that binds to the target, wherein the second antibody is linked to streptavidin or biotin, wherein the emulsion library is useful for an ELISA assay to detect an antigen from a sample but the droplets do not contain the antigen or the sample and further wherein the emulsion library includes beads labeled with a plurality of optical labels, wherein each of the plurality of optical labels comprises a dye of increasing concentration from one label to the next.

8. The emulsion library of claim 7 , wherein the bead in each droplet comprises a magnetic bead.

9. The emulsion library of claim 7 , wherein the first antibody and the second antibody form an enzyme-linked immunosorbent assay (ELISA) sandwich when the aqueous droplets are introduced to the sample comprising the antigen and an enzyme linked to biotin or streptavidin.

10. The emulsion library of claim 7 , wherein the surfactant is a block copolymer consisting of one or more perfluorinated polyether (PFPE) blocks and one or more polyethylene glycol (PEG) blocks such that the stable aqueous droplets are capable of storage for at least 30 days at temperature between about −70 and 95 degrees C. without coalescing spontaneously with droplet stability that prevents cross-contamination of antibody pairs.

11. The emulsion library of claim 7 , wherein aqueous droplets have been stored have been stored for a period greater than 90 days under conditions that tend to promote droplet coalescence and wherein the droplets have a stable shelf life, and do not coalesce over the period greater than 90 days.

12. The emulsion library of claim 7 , wherein the oil and the surfactant are selected to prevent the exchanges of contents among the droplets during the storage and wherein the droplets do not coalesce during storage.

13. The emulsion library of claim 7 , wherein the droplets are collected and stored in a vial and do not contain the antigen or the sample in the vial.

14. The emulsion library of claim 13 , wherein the vial comprises two holes including a first oil fill/air purge hole and a second emulsion introduction hole.

15. The emulsion library of claim 7 , wherein the emulsion library includes at least eight distinct optical labels distinguishable by an increasing concentration of fluorescent dye such that when a histogram of maximum fluorescence intensity data from the droplets is built, the histogram includes uniformly separated peaks across one decade when plotted on a log scale.

16. The emulsion library of claim 7 , wherein first antibody and the second antibody are useful for an ELISA assay to detect a target antigen in a tumor biopsy sample, and further wherein the droplets are stored in a vial, and the droplets do not contain the target antigen or the tumor biopsy sample in the vial.

Assignees

• Bio Rad Laboratories Inc

Inventors

• Link Darren
• Hutchison John Brian
• Samuels Michael
• Weiner Michael

Classifications

• B01J2219/0074

  Biological products · CPC title

• B01J2219/00743

  Cells · CPC title

• G01N15/1459

  the analysis being performed on a sample stream · CPC title

• B01J2219/00702

  Processes involving means for analysing and characterising the products · CPC title

• B01J2219/00722

  Nucleotides · CPC title