Formation and control of fluidic species

Patent metadata

Field	Value
Publication number	US-9038919-B2
Application number	US-24691105-A
Country	US
Kind code	B2
Filing date	Oct 7, 2005
Priority date	Apr 10, 2003
Publication date	May 26, 2015
Grant date	May 26, 2015

Abstract

This invention generally relates to systems and methods for the formation and/or control of fluidic species, and articles produced by such systems and methods. In some cases, the invention involves unique fluid channels, systems, controls, and/or restrictions, and combinations thereof. In certain embodiments, the invention allows fluidic streams (which can be continuous or discontinuous, i.e., droplets) to be formed and/or combined, at a variety of scales, including microfluidic scales. In one set of embodiments, a fluidic stream may be produced from a channel, where a cross-sectional dimension of the fluidic stream is smaller than that of the channel, for example, through the use of structural elements, other fluids, and/or applied external fields, etc. In some cases, a Taylor cone may be produced. In another set of embodiments, a fluidic stream may be manipulated in some fashion, for example, to create tubes (which may be hollow or solid), droplets, nested tubes or droplets, arrays of tubes or droplets, meshes of tubes, etc. In some cases, droplets produced using certain embodiments of the invention may be charged or substantially charged, which may allow their further manipulation, for instance, using applied external fields. Non-limiting examples of such manipulations include producing charged droplets, coalescing droplets (especially at the microscale), synchronizing droplet formation, aligning molecules within the droplet, etc. In some cases, the droplets and/or the fluidic streams may include colloids, cells, therapeutic agents, and the like.

First claim

What is claimed is:

1. A method of producing a plurality of droplets, comprising:
flowing a first liquid in a first microfluidic channel and a second liquid in a second microfluidic channel such that the first and the second liquids meet at a junction of the first and second microfluidic channels; and
producing a plurality of regularly-spaced, single-file droplets comprising the first liquid at the junction by applying an electric field to the first and second liquids as they meet at the junction, wherein the droplets are surrounded by the second liquid and flow within at least one of the microfluidic channels.

2. The method of claim 1 , wherein said first liquid is an aqueous liquid and said second liquid is a liquid that is immiscible in said aqueous liquid.

3. The method of claim 1 , wherein said droplets have an average diameter of less than about 60 microns.

4. The method of claim 1 , wherein said first microfluidic channel and said second microfluidic channel are contained on a device comprising a location for expelling said droplets.

5. The method of claim 1 , further comprising the step of conducting a reaction in said droplets.

6. The method of claim 1 , wherein said first liquid forms a Taylor cone when expelled from an outlet in said first microfluidic channel.

7. The method of claim 6 , wherein the first and second liquids flow through a dimensional restriction that stabilizes the Taylor cone.

8. The method of claim 1 , wherein the droplets comprise a homogeneous distribution of diameters.

Assignees

• Link Darren Roy
• Weitz David A
• Marquez-Sanchez Manuel
• Cheng Zhengdong
• Harvard College

Inventors

• Link Darren Roy
• Weitz David A
• Marquez-Sanchez Manuel
• Cheng Zhengdong

Classifications

• B29B9/10

  by moulding the material, i.e. treating it in the molten state · CPC title

• B29B2009/125

  Micropellets, microgranules, microparticles · CPC title

• B05B7/061

  with several liquid outlets discharging one or several liquids · CPC title

• B01L3/502715

  characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces · CPC title

• B01J2/04 Primary

  in a gaseous medium {(if combined with suspending the material in a gas, e.g. fluidised beds B01J2/16)} · CPC title