Gellan gum with double setting temperatures and the preparation method and use thereof

Patent metadata

Field	Value
Publication number	US-11390694-B2
Application number	US-201816495345-A
Country	US
Kind code	B2
Filing date	Mar 29, 2018
Priority date	Mar 30, 2017
Publication date	Jul 19, 2022
Grant date	Jul 19, 2022

Abstract

A gellan gum product having double setting temperatures, its preparation method and use thereof are provided. The obtained product has low setting temperatures, and the provided preparation method has the advantages of simple pretreatment of fermentation broth, shortened production cycle and direct extraction of such gellan gum product from the fermentation broth

First claim

The invention claimed is:

1. A gellan gum, wherein the gellan gum has double setting temperatures measured without adjusting concentration of the gellan gum; wherein a first setting temperature is in the range of 30° C. to 40° C., and a second setting temperature is in the range of 40° C. to 65° C.; and wherein the first setting temperature and the second setting temperature are different.

2. A method for preparing the gellan gum according to claim 1 , wherein the method comprises the following steps:
(1) pretreatment of gellan gum fermentation broth: adding an alkali metal salt into gellan gum fermentation broth, and stirring to obtain pretreated fermentation broth;
(2) deacylation of fermentation broth: adjusting pH of the pretreated fermentation broth with an alkali, stirring, adjusting pH back with an acid, and stirring to obtain deacylated fermentation broth;
(3) removal of metal ions from fermentation broth: adding a metal ion chelating agent into the deacylated fermentation broth, and stirring to obtain fermentation broth with metal ions removed;
(4) pH adjustment back of fermentation broth: adjusting back pH of the fermentation broth with metal ions removed with an acid, and stirring to obtain fermentation broth with pH adjusted back;
(5) heating of fermentation broth: heating the fermentation broth with pH adjusted back of step (4), and keeping the temperature to obtain hot gel solution; and
(6) alcohol precipitation, separation, drying and milling of fermentation broth: precipitating the hot gel solution of step (5) by using alcohol, separating, drying, and milling to obtain the gellan gum.

3. The method according to claim 2 , wherein said alkali metal salt of step (1) is a divalent alkali metal salt, monovalent alkali metal salt, or a combination thereof.

4. The method according to claim 2 , wherein said alkali of step (2) comprises one or more alkali selected from the group consisting of sodium hydroxide, potassium hydroxide, sodium carbonate, and calcium hydroxide.

5. The method according to claim 2 , wherein said acid of step (2) comprises one or more acid selected from the group consisting of hydrochloric acid, nitric acid, citric acid, sulfuric acid, oxalic acid, and phosphoric acid.

6. The method according to claim 2 , wherein said metal ion chelating agent of step (3) comprises one or more metal ion chelating agent selected from the group consisting of sodium citrate, potassium citrate, citric acid, ethylenediaminetetraacetic acid disodium salt, ethylenediaminetetraacetic acid potassium salt, ethylenediaminetetraacetic acid tetrasodium salt, ethylenediaminetetraacetic acid, sodium hexametaphosphate, potassium hexametaphosphate, sodium polyphosphate, potassium polyphosphate, potassium pyrophosphate, sodium pyrophosphate, potassium phosphate monobasic, disodium phosphate, trisodium phosphate, sodium carbonate, sodium bicarbonate, potassium bicarbonate, cation ion exchange resin, ethylene ethylenediamine hydrochloride, diamine diacetate, and lithium ethylenediamine.

7. The method according to claim 2 , wherein said acid of step (4) comprises one or more acid selected from the group consisting of hydrochloric acid, nitric acid, citric acid, sulfuric acid, oxalic acid, and phosphoric acid.

8. A method for preparing the gellan gum according to claim 1 , wherein the method comprises following steps:
(1) pretreatment of gellan gum fermentation broth: adding an alkali metal salt to gellan gum fermentation broth, stirring, adjusting pH with an alkali, stirring again, and performing solid-liquid separation to obtain solid fiber materials;
(2) washing solid fiber materials with alcohol: putting the solid fiber materials of step (1) into alcohol, adjusting pH with an acid, stirring, and performing solid-liquid separation to obtain washed solid fiber materials;
(3) dissolving solid fiber materials: dissolving the washed solid fiber materials of step (2) into hot water, adding a metal ion chelating agent, and heating to obtain hot gel solution;
(4) adjusting pH back and keeping temperature: adjusting pH of the hot gel solution of step (3) with an acid, stirring, and keeping temperature to obtain hot gel solution with pH adjusted back; and
(5) alcohol precipitation, separation, drying, and milling: precipitating the hot gel solution with pH adjusted back of step (4) by using alcohol, separating, drying, and milling to obtain the gellan gum.

9. The method according to claim 8 , wherein said alkali metal salt of step (1) is a divalent alkali metal salt.

10. The method according to claim 8 , wherein said alkali of step (1) comprises one or more alkali selected from the group consisting of sodium hydroxide, potassium hydroxide, sodium carbonate, and calcium hydroxide.

11. The method according to claim 8 , wherein said acid of step (2) comprises one or more acid selected from the group consisting of hydrochloric acid, nitric acid, citric acid, sulfuric acid, oxalic acid, and phosphoric acid.

12. The method according to claim 8 , wherein said metal ion chelating agent of step (3) comprises one or more metal ion chelating agent selected from the group consisting of sodium citrate, potassium citrate, citric acid, ethylenediaminetetraacetic acid disodium salt, ethylenediaminetetraacetic acid potassium salt, ethylenediaminetetraacetic acid tetrasodium salt, ethylenediaminetetraacetic acid, sodium hexametaphosphate, potassium hexametaphosphate, sodium polyphosphate, potassium polyphosphate, potassium pyrophosphate, sodium pyrophosphate, potassium phosphate monobasic, disodium phosphate, trisodium phosphate, sodium carbonate, sodium bicarbonate, potassium bicarbonate, cation ion exchange resin, ethylene ethylenediamine hydrochloride, diamine diacetate, and lithium ethylenediamine.

13. The method according to claim 8 , wherein said acid of step (4) comprises one or more acid selected from the group consisting of hydrochloric acid, nitric acid, citric acid, sulfuric acid, and oxalic acid.

14. A method for producing a foodstuff comprising the step of mixing the gellan gum according to claim 1 , wherein the foodstuff is selected from a group consisting of a desert, a dysphagia food, a dairy product, and a meat product.

15. A gellan gum, wherein the gellan gum has double setting temperatures measured without adjusting concentration of the gellan gum, wherein a first setting temperature and a second setting temperature are both in a range of 30° C. to 50° C., and wherein the first setting temperature and the second setting temperature are different.

16. A composition comprising the gellan gum according to claim 1 , wherein the gellan gum is present in a foodstuff.

17. A composition comprising the gellan gum according to claim 15 , wherein the gellan gum is present in a foodstuff.

18. The composition of claim 16 , wherein the foodstuff is selected from a group consisting of a dessert, a dysphagia food, a dairy product, and a meat product.

19. The composition of claim 17 , wherein the foodstuff is selected from a group consisting of a dessert, a dysphagia food, a dairy product, and a meat product.

20. The composition of claim 18 , wherein the dessert is a chocolate paste or mousse.

21. The composition of claim 19 , wherein the dessert is a chocolate paste or mousse.

Assignees

• Dsm Ip Assets Bv

Inventors

• Yuan Chienkuo
• Zhao Jie
• Liu Guojun

Classifications

• C08B37/006 Primary

  Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof · CPC title

• C08B37/0003 Primary

  General processes for their isolation or fractionation, e.g. purification or extraction from biomass · CPC title

• A23C9/154

  containing thickening substances, eggs or cereal preparations; Milk gels · CPC title

• A23L29/272 Primary

  Gellan · CPC title

• A23C9/137

  Thickening substances · CPC title