Transgenic plants having increased tolerance to aluminum

Patent metadata

Field	Value
Publication number	US-11339403-B2
Application number	US-201916575915-A
Country	US
Kind code	B2
Filing date	Sep 19, 2019
Priority date	Nov 2, 2011
Publication date	May 24, 2022
Grant date	May 24, 2022

Abstract

Methods and materials for modulating aluminum tolerance in plants are disclosed. For example, nucleic acids encoding aluminum tolerance-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased tolerance to aluminum and methods of increasing plant yield in soil containing elevated levels of aluminum.

First claim

What is claimed is:

1. A method of increasing plant yield in soil containing elevated levels of Al 3+ , said method comprising growing a monocotyledonous plant comprising an exogenous nucleic acid on soil having an elevated level of Al 3+ , said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence encoding a polypeptide having 95 percent or greater sequence identity to the amino acid sequence set forth in SEQ ID NO:237, and selecting the monocotyledonous plant for having increased yield when grown in soil containing the elevated level of Al 3+ as compared to the corresponding yield of a control plant that does not comprise said nucleic acid, wherein the regulatory region is heterologous with respect to the nucleotide sequence.

2. The method of claim 1 , wherein said nucleotide sequence encodes a polypeptide having 97 percent or greater sequence identity to said amino acid sequence set forth in SEQ ID NO:237.

3. A method of increasing plant yield in soil containing elevated levels of Al 3+ , said method comprising growing a monocotyledonous plant comprising an exogenous nucleic acid on soil having an elevated level of Al 3+ , said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence having 95 percent or greater sequence identity to the nucleotide sequence set forth in SEQ ID NO:236,
selecting the monocotyledonous plant for having increased yield when grown in soil containing the elevated level of Al 3+ as compared to the corresponding yield of a control plant that does not comprise said nucleic acid, wherein the regulatory region is heterologous with respect to the nucleotide sequence.

4. The method of claim 3 , wherein said nucleotide sequence has 97 percent or greater sequence identity to the nucleotide sequence set forth in SEQ ID NO:236.

5. The method of claim 1 , wherein said nucleotide sequence comprises the nucleotide sequence set forth in SEQ ID NO:236, or wherein said nucleotide sequence encodes the polypeptide set forth in SEQ ID NO:237.

6. The method of claim 1 wherein said regulatory region is a promoter.

7. The method of claim 6 , wherein said promoter is selected from the group consisting of YP0092, PT0676, PT0708, PT0613, PT0672, PT0678, PT0688, PT0837, the napin promoter, the Arcelin-5 promoter, the phaseolin gene promoter, the soybean trypsin inhibitor promoter, the ACP promoter, the stearoyl-ACP desaturase gene promoter, the soybean α′ subunit of β-conglycinin promoter, the oleosin promoter, the 15 kD zein promoter, the 16 kD zein promoter, the 19 kD zein promoter, the 22 kD zein promoter, the 27 kD zein promoter, the Osgt-1 promoter, the beta-amylase gene promoter, the barley hordein gene promoter, p326, YP0144, YP190, p13879, YP0050, p32449, 21876, YP0158, YP0214, YP0380, PT0848, PT0633, the cauliflower mosaic virus (CaMV) 35S promoter, the mannopine synthase (MAS) promoter, the 1′ or 2′ promoters derived from T-DNA of Agrobacterium tumefaciens , the figwort mosaic virus 34S promoter, rice actin promoter, maize ubiquitin-1 promoter, ribulose-1,5-bisphosphate carboxylase (RbcS) promoter, the pine cab6 promoter, the Cab-1 gene promoter from wheat, the CAB-1 promoter from spinach, the cablR promoter from rice, the pyruvate orthophosphate dikinase (PPDK) promoter from corn, the tobacco Lhcbl*2 promoter, the Arabidopsis thaliana SUC2 sucrose-H+ symporter promoter, and a thylakoid membrane protein promoter from spinach, and PT0585.

8. The method of claim 1 , wherein said plant comprising said exogenous nucleic acid has an improved growth rate relative to a corresponding plant that does not comprise said nucleic acid.

9. The method of claim 1 , wherein said plant comprising said exogenous nucleic acid has improved vegetative growth relative to a corresponding plant that does not comprise said nucleic acid.

10. A method of increasing tolerance of a monocotyledonous plant to elevated levels of aluminum, said method comprising
a) introducing into a plurality of monocotyledonous plant cells an exogenous nucleic acid comprising a regulatory region operably linked to a nucleic acid sequence encoding a polypeptide having 95 percent or greater sequence identity to an amino acid sequence set forth in SEQ ID NO:237, wherein the regulatory region is heterologous with respect to the nucleotide sequence;
b) producing monocotyledonous plants from said plant cells;
c) growing said monocotyledonous plants on soil having an elevated level of Al 3+ ; and
d) selecting a monocotyledonous plant for having increased yield when grown in soil containing the elevated level of Al 3+ as compared to that of a control plant that does not comprise said nucleic acid.

11. A method of increasing tolerance of a monocotyledonous plant to elevated levels of aluminum, said method comprising
a) introducing into a plurality of plant cells an isolated nucleic acid comprising a regulatory region operably linked to a nucleic acid sequence encoding a polypeptide having 95 percent or greater sequence identity to an amino acid sequence set forth in SEQ ID NO:237; and
b) selecting a monocotyledonous plant produced from said plurality of plant cells that has an increased tolerance to an elevated level of Al 3+ when grown in soil containing the elevated level of Al 3+ as compared to the tolerance in a corresponding control plant that does not comprise said isolated nucleic acid.

Assignees

• Ceres Inc

Inventors

• Sosa Julissa
• Wang Wuyi

Classifications

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• A23L5/00

  Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor · CPC title

• A23L25/00

  Food consisting mainly of nutmeat or seeds; Preparation or treatment thereof · CPC title

• C07K14/415

  from plants · CPC title

• A23L7/10

  Cereal-derived products · CPC title