P450-BM3 variants with improved activity
US-10982197-B2 · Apr 20, 2021 · US
P450-BM3 variants with improved activity
US11279917B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11279917-B2 |
| Application number | US-202015931310-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 13, 2020 |
| Priority date | Jul 9, 2014 |
| Publication date | Mar 22, 2022 |
| Grant date | Mar 22, 2022 |
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- Title
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Abstract
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The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.
First claim
Opening claim text (preview).
What is claimed is: 1. A recombinant cytochrome P450-BM3 variant having cytochrome P450-BM3 activity and comprising the amino acid sequence of SEQ ID NO: 48. 2. The recombinant cytochrome P450-BM3 variant of claim 1 , wherein said variant oxidizes at least three organic substrates. 3. The recombinant cytochrome P450-BM3 variant of claim 2 , wherein the at least three organic substrates are selected from the group consisting of nifedipine, propranolol, verapamil, and diclofenac. 4. An isolated polynucleotide comprising a polynucleotide sequence encoding the recombinant cytochrome P450-BM3 variant of claim 1 . 5. The isolated polynucleotide of claim 4 , wherein said polynucleotide comprises the polynucleotide sequence of SEQ ID NO: 47. 6. An expression vector comprising the polynucleotide of claim 4 . 7. The vector of claim 6 , wherein said polynucleotide sequence is operably linked with regulatory sequences suitable for expression of said polynucleotide sequence in a suitable host cell. 8. The vector of claim 7 , wherein said host cell is a prokaryotic or eukaryotic cell. 9. The vector of claim 8 , wherein said host cell is a prokaryotic cell. 10. The vector of claim 8 , wherein said host cell is E. coli. 11. An isolated host cell comprising the vector of claim 6 . 12. A method for producing at least one recombinant cytochrome P450-BM3 variant comprising culturing the host cell of claim 11 under conditions such that the recombinant cytochrome P450-BM3 variant is produced. 13. The method of claim 12 , further comprising the step of recovering said at least one recombinant cytochrome P450-BM3 variant.
Assignees
Inventors
Classifications
- C12P13/001Primary
Amines; Imines · CPC title
NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase · CPC title
- C12N9/0071Primary
acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title
Cytochromes · CPC title
NADPH-cytochrome P450 reductase (1.6.2.4) · CPC title
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- What does patent US11279917B2 cover?
- The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.
- Who is the assignee on this patent?
- Codexis Inc
- What technology area does this patent fall under?
- Primary CPC classification C12P13/001. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 22 2022 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 7 related publications on this page (citations in our corpus or others sharing the same primary CPC).