P450-BM3 variants with improved activity
US-9683220-B2 · Jun 20, 2017 · US
P450-BM3 variants with improved activity
US10100289B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10100289-B2 |
| Application number | US-201715619176-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 9, 2017 |
| Priority date | Jul 9, 2014 |
| Publication date | Oct 16, 2018 |
| Grant date | Oct 16, 2018 |
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- Title
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Abstract
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The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.
First claim
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What is claimed is: 1. A recombinant cytochrome P450-BM3 variant comprising the polypeptide sequence set forth in SEQ ID NO: 22, wherein said recombinant cytochrome P450-BM3 variant oxidizes at least three organic substrates. 2. The recombinant cytochrome P450-BM3 variant of claim 1 , wherein said organic substrate is selected from nifedipine, propranolol, verapamil, and diclofenac. 3. An isolated polynucleotide comprising a polynucleotide sequence encoding the recombinant cytochrome P450-BM3 variant of claim 1 . 4. The isolated polynucleotide of claim 3 , wherein said polynucleotide comprises the nucleotide sequence of SEQ ID NO: 21. 5. An expression vector comprising the polynucleotide of claim 3 . 6. The vector of claim 5 , wherein said polynucleotide is operably linked with regulatory sequences suitable for expression of said polynucleotide sequence in a suitable host cell. 7. The vector of claim 6 , wherein said host cell is a prokaryotic cell. 8. The vector of claim 7 , wherein said host cell is E. coli. 9. A host cell comprising the vector of claim 5 . 10. A method for producing a recombinant cytochrome P450-BM3 variant comprising culturing the host cell of claim 9 under conditions such that a recombinant cytochrome P450-BM3 variant is produced. 11. The method of claim 10 , further comprising the step of recovering said recombinant cytochrome P450-BM3 variant.
Assignees
Inventors
Classifications
- C12P13/001Primary
Amines; Imines · CPC title
NADPH-cytochrome P450 reductase (1.6.2.4) · CPC title
- C12N9/0071Primary
acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title
Unspecific monooxygenase (1.14.14.1) · CPC title
Cytochromes · CPC title
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- What does patent US10100289B2 cover?
- The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.
- Who is the assignee on this patent?
- Codexis Inc
- What technology area does this patent fall under?
- Primary CPC classification C12P13/001. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Oct 16 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).