P450-BM3 variants with improved activity
US-10450550-B2 · Oct 22, 2019 · US
P450-BM3 variants with improved activity
US10704030B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10704030-B2 |
| Application number | US-201916562673-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 6, 2019 |
| Priority date | Jul 7, 2015 |
| Publication date | Jul 7, 2020 |
| Grant date | Jul 7, 2020 |
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- Title
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Abstract
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The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.
First claim
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What is claimed is: 1. A recombinant cytochrome P450-BM3 variant comprising the polypeptide sequence set forth in SEQ ID NO: 16. 2. The recombinant cytochrome P450-BM3 variant of claim 1 , wherein said variant oxidizes at least three organic substrates. 3. The recombinant cytochrome P450-BM3 variant of claim 2 , wherein said organic substrate is selected from loratadine, imatinib, geftinib, and diclofenac. 4. A recombinant polynucleotide sequence wherein said sequence comprises SEQ ID NO: 15. 5. An expression vector comprising the polynucleotide sequence of claim 4 . 6. The vector of claim 5 , wherein said polynucleotide sequence is operably linked with regulatory sequences suitable for expression of said polynucleotide sequence in a suitable host cell. 7. The vector of claim 6 , wherein said host cell is a prokaryotic or eukaryotic cell. 8. The vector of claim 7 , wherein said host cell is a prokaryotic cell. 9. The vector of claim 8 , wherein said host cell is E. coli. 10. A host cell comprising the vector of claim 5 . 11. A method for producing at least one recombinant cytochrome P450-BM3 variant comprising culturing a host cell under conditions such that at least one recombinant cytochrome P450-BM3 variant of claim 1 is produced by said host cell. 12. The method of claim 11 , further comprising the step of recovering said at least one recombinant cytochrome P450-BM3 variant.
Assignees
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Classifications
Unspecific monooxygenase (1.14.14.1) · CPC title
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression · CPC title
Bacteria; Culture media therefor · CPC title
Yeasts; Culture media therefor · CPC title
Genes encoding for enzymes or proenzymes · CPC title
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Frequently asked questions
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- What does patent US10704030B2 cover?
- The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.
- Who is the assignee on this patent?
- Codexis Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/0042. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jul 07 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 5 related publications on this page (citations in our corpus or others sharing the same primary CPC).