Aqueous extracts of Eruca sativa leaves and methods for inhibiting growth of gram-positive bacteria and mycoplasma

Patent metadata

Field	Value
Publication number	US-11191800-B2
Application number	US-201816149674-A
Country	US
Kind code	B2
Filing date	Oct 2, 2018
Priority date	Oct 2, 2018
Publication date	Dec 7, 2021
Grant date	Dec 7, 2021

Abstract

The invention is directed to an aqueous extract of Eruca sativa (arugula) leaves that has antimicrobial activity on Gram-positive bacteria and mycoplasmas. The extract may be purified away from solid or insoluble components, standardized based on the weight of its non-aqueous or solid content, assayed for antimicrobial activity, and provided in an aseptic or sterile form for pharmaceutical use. It may be used to kill or inhibit the growth of microorganisms such as Gram positive bacteria, promote wound healing, or as prophylaxis against host colonization or infection by a microorganism.

First claim

The invention claimed is:

1. A method for inhibiting the growth of a Gram positive bacterium, comprising:
preparing an aqueous Eruca sativa extract by:
grinding a sample consisting of dried leaves of a Eruca sativa plant,
macerating the ground leaves of the Eruca sativa plant with an extractant consisting of water to extract the leaves of the Eruca sativa plant for a period of days at a temperature of about 25° C. to extract water-soluble components from the leaves of the Eruca sativa plant then filtering to form a first extract,
concentrating the first extract under vacuum to produce the aqueous Eruca saliva extract in the form of a thick concentrated extract liquid, then
dispensing an aqueous composition comprising the thick concentrated extract liquid from a dispenser in the form of a foam,

contacting the Gram positive bacterium with the foam to inhibit the growth of the Gram positive bacterium, wherein the Gram positive bacterium is Cellulomonas strain B22.

2. The method of claim 1 , wherein the macerating is performed at a pH ranging from 6.0 to 6.5.

3. The method of claim 1 , wherein the foam contains at least 0.15 wt % of an Eruca sativa aqueous extract based on anhydrous weight or dry weight of the Eruca sativa extract.

4. The method of claim 1 , wherein contacting the Gram positive bacterium occurs in a skin, nail, or hair wound or injury.

5. The method of claim 1 , wherein contacting the Gram positive bacterium occurs in a wound or injury to a mucous membrane.

Assignees

• Univ Imam Abdulrahman Bin Faisal

Inventors

• Hammami Ines Mohsen
• Alghamdi Azzah Ibrahim

Classifications

• A61K2236/00

  Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine · CPC title

• A61K36/31 Primary

  Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi · CPC title

• A61P31/04 Primary

  Antibacterial agents · CPC title