Binding probe circuits for molecular sensors

Patent metadata

Field	Value
Publication number	US-11143617-B2
Application number	US-202016831722-A
Country	US
Kind code	B2
Filing date	Mar 26, 2020
Priority date	May 9, 2017
Publication date	Oct 12, 2021
Grant date	Oct 12, 2021

Abstract

In various embodiments a molecular circuit is disclosed. The circuit comprises a negative electrode, a positive electrode spaced apart from the negative electrode, and a binding probe molecule conductively attached to both the positive and negative electrodes to form a circuit having a conduction pathway through the binding probe. In various examples, the binding probe is an antibody, the Fab domain of an antibody, a protein, a nucleic acid oligomer hybridization probe, or an aptamer. The circuit may further comprise molecular arms used to wire the binding probe to the electrodes. In various embodiments, the circuit functions as a sensor wherein electrical signals, such as changes to voltage, current, impedance, conductance, or resistance in the circuit, are measured as targets interact with the binding probe. In various embodiments, the circuit provides a means to measure the presence, absence, or concentration of an analyte in a solution.

First claim

We claim:

1. A circuit comprising:
a first electrode;
a second electrode spaced apart from the first electrode by a gap; and

a binding probe electrically connected to the first electrode by a first peptide arm molecule and electrically connected to the second electrode by a second peptide arm molecule, wherein the first peptide arm molecule is attached to the first electrode and the second peptide arm molecule is attached to the second electrode by material binding peptide-metal interactions.

2. The circuit of claim 1 , wherein the binding probe comprises a single stranded DNA oligonucleotide or a single stranded RNA oligonucleotide.

3. The circuit of claim 1 , wherein the circuit is configured to sense information on the presence of, or a concentration of, a target DNA or RNA molecule.

4. The circuit of claim 1 , wherein the first electrode and the second electrode comprise a source and drain electrode pair.

5. The circuit of claim 1 , further comprising a gate electrode.

6. The circuit of claim 5 , wherein the gate electrode is positioned under the gap, insulated from the first and second electrodes.

7. The circuit of claim 1 , wherein the circuit resides on a sensor array electronic measurement chip with an array of sensor pixel electronic elements, wherein the sensor pixel electronic elements monitor an electrical change from a single circuit.

8. The circuit of claim 7 , wherein the sensor array electronic measurement chip comprises a CMOS chip.

Assignees

• Roswell Biotechnologies Inc

Inventors

• Merriman Barry L
• Alagarswamy Govindaraj Venkatesh
• Mola Paul
• Geiser Tim
• Costa Gina

Classifications

• C12Q1/6869

  Methods for sequencing · CPC title

• G01N27/3275 Primary

  Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction · CPC title

• G01N33/5438 Primary

  Electrodes · CPC title

• G01N27/3276

  being a hybridisation with immobilised receptors (using a FET type sensor G01N27/4145; concerning the hybridisation C12Q1/68) · CPC title

• G01N27/4145

  specially adapted for biomolecules, e.g. gate electrode with immobilised receptors · CPC title