Transaminase biocatalysts
US-10160985-B2 · Dec 25, 2018 · US
Transaminase biocatalysts
US11078505B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11078505-B2 |
| Application number | US-202016807977-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 3, 2020 |
| Priority date | Feb 26, 2009 |
| Publication date | Aug 3, 2021 |
| Grant date | Aug 3, 2021 |
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- Title
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- Abstract
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Abstract
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The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
First claim
Opening claim text (preview).
What is claimed is: 1. A recombinant transaminase polypeptide comprising an amino acid at least 95% sequence identical to SEQ ID NO: 2 having transaminase activity, wherein the amino acid sequence of the recombinant transaminase polypeptide comprises residue difference as compared to SEQ ID NO: 2 at residue positions X62, X69 and X169. 2. The recombinant transaminase polypeptide of claim 1 , wherein said recombinant transaminase polypeptide is capable of converting 4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro[1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl]-1-(2,4,5-trifluorophenyl)butan-2-one (“ketoamide substrate”) to (2R)-4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro[1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl]-1-(2,4,5-trifluorophenyl)butan-2-amine (“product”) under a defined reaction condition in the presence of an amino group donor isopropylamine to levels of the product detectable by HPLC-UV at 210 nm, where the reaction condition comprises about 2 g/L ketoamide substrate, about 0.5 M isopropylamine, about 22° C., about pH 7.5, about 5% DMSO, about 100 μM pyridoxal phosphate, and about 20 mg/mL of transaminase polypeptide. 3. The recombinant transaminase polypeptide of claim 1 , wherein said recombinant transaminase polypeptide is capable of converting the ketoamide substrate to the product with an activity that is equal to or greater than the activity of the polypeptide of SEQ ID NO: 4 under the defined reaction condition. 4. The recombinant transaminase polypeptide of claim 1 , wherein said recombinant transaminase polypeptide is capable of converting the ketoamide substrate to product in at least 90% enantiomeric excess. 5. The recombinant transaminase polypeptide of claim 1 , wherein said recombinant transaminase polypeptide is capable of converting the ketoamide substrate to product in at least 99% enantiomeric excess.
Assignees
Inventors
Classifications
Genes encoding for enzymes or proenzymes · CPC title
using catalysts, e.g. selective catalysts · CPC title
DNA sequences coding for fusion proteins · CPC title
transferring nitrogenous groups (2.6) · CPC title
Beta-alanine-pyruvate transaminase (2.6.1.18) · CPC title
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Frequently asked questions
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- What does patent US11078505B2 cover?
- The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
- Who is the assignee on this patent?
- Codexis Inc
- What technology area does this patent fall under?
- Primary CPC classification C12P17/182. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Aug 03 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 5 related publications on this page (citations in our corpus or others sharing the same primary CPC).