Enzymatically produced cellulose
US-2020080121-A1 · Mar 12, 2020 · US
Enzymatically produced cellulose
US10927392B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10927392-B2 |
| Application number | US-201916549092-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 23, 2019 |
| Priority date | Dec 23, 2014 |
| Publication date | Feb 23, 2021 |
| Grant date | Feb 23, 2021 |
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- Title
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Abstract
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Enzymatic reactions are disclosed herein comprising water, glucose-1-phosphate, cellodextrin, and at least one cellodextrin phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6. These reactions produce a low molecular weight, insoluble cellulose with enhanced features.
First claim
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What is claimed is: 1. An isolated composition comprising: (i) insoluble cellulose having a weight-average degree of polymerization (DPw) of at least 15, (ii) a cellodextrin phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6, and (iii) a starch phosphorylase or sucrose phosphorylase. 2. The isolated composition of claim 1 , wherein the insoluble cellulose has a DPw of at least 17. 3. The isolated composition of claim 1 , wherein the insoluble cellulose has a DPw of 15 to 50. 4. The isolated composition of claim 1 , wherein the insoluble cellulose has a DPw of 15 to 30. 5. The isolated composition of claim 1 , wherein the insoluble cellulose has a DPw of 17 to 50. 6. The isolated composition of claim 1 , wherein the insoluble cellulose has a DPw of 17 to 30. 7. The isolated composition of claim 1 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6. 8. The isolated composition of claim 2 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6. 9. The isolated composition of claim 3 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6. 10. The isolated composition of claim 1 , wherein the isolated composition comprises the sucrose phosphorylase. 11. The isolated composition of claim 1 , wherein the isolated composition comprises the starch phosphorylase. 12. The isolated composition of claim 11 , wherein the isolated composition further comprises a starch debranching enzyme. 13. The isolated composition of claim 12 , wherein the starch debranching enzyme is a pullulanase or isoamylase. 14. The isolated composition of claim 1 , wherein the isolated composition is a food. 15. A method of producing insoluble cellulose in a composition, said method comprising: combining, in a composition, at least water, cellodextrin, a cellodextrin phosphorylase enzyme, and (i) starch, inorganic phosphate and a starch phosphorylase, or (ii) sucrose, inorganic phosphate and a sucrose phosphorylase, wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6, wherein insoluble cellulose having a weight-average degree of polymerization (DPw) of at least 15 is produced by the cellodextrin phosphorylase enzyme in the composition. 16. The method of claim 15 , wherein the cellodextrin comprises cellobiose. 17. The method of claim 15 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6. 18. The method of claim 15 , wherein the insoluble cellulose produced in the composition has a DPw of at least 17. 19. The method of claim 15 , wherein the insoluble cellulose produced in the composition has a DPw of 15 to 50. 20. The method of claim 15 , wherein the insoluble cellulose produced in the composition has a DPw of 17 to 50. 21. The method of claim 15 , wherein the composition is a food.
Assignees
Inventors
Classifications
- C12P19/04Primary
Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds · CPC title
Hexosyltransferases (2.4.1) · CPC title
Emollients or protectives, e.g. against radiation · CPC title
- C12P19/18Primary
produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins · CPC title
Cellodextrin phosphorylase (2.4.1.49) · CPC title
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Frequently asked questions
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- What does patent US10927392B2 cover?
- Enzymatic reactions are disclosed herein comprising water, glucose-1-phosphate, cellodextrin, and at least one cellodextrin phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6. These reactions produce a low molecular weight, insoluble cellulose with enhanced features.
- Who is the assignee on this patent?
- Dupont Ind Biosciences Usa Llc
- What technology area does this patent fall under?
- Primary CPC classification C12P19/04. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 23 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 3 related publications on this page (citations in our corpus or others sharing the same primary CPC).