Enzymatically produced cellulose
US-10392641-B2 · Aug 27, 2019 · US
US2020080121A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2020080121-A1 |
| Application number | US-201916549092-A |
| Country | US |
| Kind code | A1 |
| Filing date | Aug 23, 2019 |
| Priority date | Dec 23, 2014 |
| Publication date | Mar 12, 2020 |
| Grant date | — |
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Enzymatic reactions are disclosed herein comprising water, glucose-1-phosphate, cellodextrin, and at least one cellodextrin phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6. These reactions produce a low molecular weight, insoluble cellulose with enhanced features.
Opening claim text (preview).
1 - 12 . (canceled) 13 . An isolated composition comprising: (i) insoluble cellulose having a weight-average degree of polymerization (DPw) of at least 15, (ii) a cellodextrin phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6, and (iii) a starch phosphorylase or sucrose phosphorylase. 14 . The isolated composition of claim 13 , wherein the insoluble cellulose has a DPw of at least 17. 15 . The isolated composition of claim 13 , wherein the insoluble cellulose has a DPw of 15 to 50. 16 . The isolated composition of claim 13 , wherein the insoluble cellulose has a DPw of 15 to 30. 17 . The isolated composition of claim 13 , wherein the insoluble cellulose has a DPw of 17 to 50. 18 . The isolated composition of claim 13 , wherein the insoluble cellulose has a DPw of 17 to 30. 19 . The isolated composition of claim 13 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6. 20 . The isolated composition of claim 14 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6. 21 . The isolated composition of claim 15 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6. 22 . The isolated composition of claim 13 , wherein the isolated composition comprises the sucrose phosphorylase. 23 . The isolated composition of claim 13 , wherein the isolated composition comprises the starch phosphorylase. 24 . The isolated composition of claim 23 , wherein the isolated composition further comprises a starch debranching enzyme. 25 . The isolated composition of claim 24 , wherein the starch debranching enzyme is a pullulanase or isoamylase. 26 . The isolated composition of claim 13 , wherein the isolated composition is a food. 27 . A method of producing insoluble cellulose in a composition, said method comprising: combining, in a composition, at least water, cellodextrin, a cellodextrin phosphorylase enzyme, and (i) starch, inorganic phosphate and a starch phosphorylase, or (ii) sucrose, inorganic phosphate and a sucrose phosphorylase, wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6, wherein insoluble cellulose having a weight-average degree of polymerization (DPw) of at least 15 is produced by the cellodextrin phosphorylase enzyme in the composition. 28 . The method of claim 27 , wherein the cellodextrin comprises cellobiose. 29 . The method of claim 27 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6. 30 . The method of claim 27 , wherein the insoluble cellulose produced in the composition has a DPw of at least 17. 31 . The method of claim 27 , wherein the insoluble cellulose produced in the composition has a DPw of 15 to 50. 32 . The method of claim 27 , wherein the insoluble cellulose produced in the composition has a DPw of 17 to 50. 33 . The method of claim 27 , wherein the composition is a food.
Emollients or protectives, e.g. against radiation · CPC title
Hexosyltransferases (2.4.1) · CPC title
Cellulose; Modified cellulose · CPC title
Cellodextrin phosphorylase (2.4.1.49) · CPC title
Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds · CPC title
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