Enzymatically produced cellulose

US2020080121A1 · US · A1

Patent metadata
FieldValue
Publication numberUS-2020080121-A1
Application numberUS-201916549092-A
CountryUS
Kind codeA1
Filing dateAug 23, 2019
Priority dateDec 23, 2014
Publication dateMar 12, 2020
Grant date

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  1. Title

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  2. Abstract

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  3. Assignees and inventors

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  4. Key dates

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  5. First independent claim

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  6. CPC / IPC classifications

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  7. Citations and related patents

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Abstract

Official abstract text for this publication.

Enzymatic reactions are disclosed herein comprising water, glucose-1-phosphate, cellodextrin, and at least one cellodextrin phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6. These reactions produce a low molecular weight, insoluble cellulose with enhanced features.

First claim

Opening claim text (preview).

1 - 12 . (canceled) 13 . An isolated composition comprising: (i) insoluble cellulose having a weight-average degree of polymerization (DPw) of at least 15, (ii) a cellodextrin phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6, and (iii) a starch phosphorylase or sucrose phosphorylase. 14 . The isolated composition of claim 13 , wherein the insoluble cellulose has a DPw of at least 17. 15 . The isolated composition of claim 13 , wherein the insoluble cellulose has a DPw of 15 to 50. 16 . The isolated composition of claim 13 , wherein the insoluble cellulose has a DPw of 15 to 30. 17 . The isolated composition of claim 13 , wherein the insoluble cellulose has a DPw of 17 to 50. 18 . The isolated composition of claim 13 , wherein the insoluble cellulose has a DPw of 17 to 30. 19 . The isolated composition of claim 13 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6. 20 . The isolated composition of claim 14 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6. 21 . The isolated composition of claim 15 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6. 22 . The isolated composition of claim 13 , wherein the isolated composition comprises the sucrose phosphorylase. 23 . The isolated composition of claim 13 , wherein the isolated composition comprises the starch phosphorylase. 24 . The isolated composition of claim 23 , wherein the isolated composition further comprises a starch debranching enzyme. 25 . The isolated composition of claim 24 , wherein the starch debranching enzyme is a pullulanase or isoamylase. 26 . The isolated composition of claim 13 , wherein the isolated composition is a food. 27 . A method of producing insoluble cellulose in a composition, said method comprising: combining, in a composition, at least water, cellodextrin, a cellodextrin phosphorylase enzyme, and (i) starch, inorganic phosphate and a starch phosphorylase, or (ii) sucrose, inorganic phosphate and a sucrose phosphorylase, wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6, wherein insoluble cellulose having a weight-average degree of polymerization (DPw) of at least 15 is produced by the cellodextrin phosphorylase enzyme in the composition. 28 . The method of claim 27 , wherein the cellodextrin comprises cellobiose. 29 . The method of claim 27 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6. 30 . The method of claim 27 , wherein the insoluble cellulose produced in the composition has a DPw of at least 17. 31 . The method of claim 27 , wherein the insoluble cellulose produced in the composition has a DPw of 15 to 50. 32 . The method of claim 27 , wherein the insoluble cellulose produced in the composition has a DPw of 17 to 50. 33 . The method of claim 27 , wherein the composition is a food.

Assignees

Inventors

Classifications

  • Emollients or protectives, e.g. against radiation · CPC title

  • Hexosyltransferases (2.4.1) · CPC title

  • Cellulose; Modified cellulose · CPC title

  • Cellodextrin phosphorylase (2.4.1.49) · CPC title

  • C12P19/04Primary

    Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds · CPC title

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What does patent US2020080121A1 cover?
Enzymatic reactions are disclosed herein comprising water, glucose-1-phosphate, cellodextrin, and at least one cellodextrin phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6. These reactions produce a low molecular weight, insoluble cellulose with enhanced features.
Who is the assignee on this patent?
Dupont Ind Biosciences Usa Llc
What technology area does this patent fall under?
Primary CPC classification C12P19/04. Mapped technology areas include Chemistry & Metallurgy.
When was this patent published?
Publication date Thu Mar 12 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 2 related publications on this page (citations in our corpus or others sharing the same primary CPC).