Enzymatically produced cellulose
US-2018334696-A1 · Nov 22, 2018 · US
US10392641B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10392641-B2 |
| Application number | US-201816191483-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 15, 2018 |
| Priority date | Dec 23, 2014 |
| Publication date | Aug 27, 2019 |
| Grant date | Aug 27, 2019 |
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Enzymatic reactions are disclosed herein comprising water, glucose-1-phosphate, cellodextrin, and at least one cellodextrin phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6. These reactions produce a low molecular weight, insoluble cellulose with enhanced features.
Opening claim text (preview).
What is claimed is: 1. An isolated composition comprising: (i) insoluble cellulose having a weight-average degree of polymerization (DPw) of at least 15, and (ii) a cellodextrin phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6. 2. The isolated composition of claim 1 , wherein the insoluble cellulose has a DPw of at least 17. 3. The isolated composition of claim 1 , wherein the insoluble cellulose has a DPw of 15 to 50. 4. The isolated composition of claim 1 , wherein the insoluble cellulose has a DPw of 15 to 30. 5. The isolated composition of claim 1 , wherein the insoluble cellulose has a DPw of 17 to 50. 6. The isolated composition of claim 1 , wherein the insoluble cellulose has a DPw of 17 to 30. 7. The isolated composition of claim 1 , wherein the insoluble cellulose has a cellulose II crystal structure. 8. The isolated composition of claim 1 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6. 9. The isolated composition of claim 2 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6. 10. The isolated composition of claim 3 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6. 11. The isolated composition of claim 4 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6. 12. The isolated composition of claim 5 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6. 13. The isolated composition of claim 6 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6. 14. The isolated composition of claim 1 , wherein the cellodextrin phosphorylase enzyme is a Vibrio species cellodextrin phosphorylase enzyme or a Ruminococcus species cellodextrin phosphorylase enzyme.
Emollients or protectives, e.g. against radiation · CPC title
Cellodextrin phosphorylase (2.4.1.49) · CPC title
Hexosyltransferases (2.4.1) · CPC title
produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins · CPC title
Cellulose; Modified cellulose · CPC title
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