Extracts and isolated compounds from cakile arabica for treatment of ulcer

Patent metadata

Field	Value
Publication number	US-10874705-B2
Application number	US-201715422614-A
Country	US
Kind code	B2
Filing date	Feb 2, 2017
Priority date	Sep 12, 2013
Publication date	Dec 29, 2020
Grant date	Dec 29, 2020

Abstract

The present invention relates to extracts, compounds isolated from Cakile arabica for use in the treatment of ulcer and to pharmaceutical compositions thereof.

First claim

The invention claimed is:

1. A method for treating an ulcer, the method comprising:
orally administering to a patient an extract from a plant material of Cakile arabica , wherein the extract includes 4-hydroxy-5-n-butoxy-5-oxopentanoic acid and tetracosanoic acid.

2. The method of claim 1 , wherein the extract is an alcoholic extract.

3. The method of claim 2 , wherein the extract is an ethanolic extract.

4. The method of claim 1 , wherein the plant material comprises the aerial parts of Cakile arabica.

5. The method of claim 1 , wherein the extract includes tetracosanoic acid and 4-hydroxy-5-n-butoxy-5-oxopentanoic acid.

6. The method of claim 1 , wherein said step of orally administering includes orally administering a pharmaceutical composition that includes the extract from a plant material of Cakile arabica.

7. The method of claim 6 , where the pharmaceutical composition is in the form of a liquid or a solid.

8. A method for treating a ulcer, the method comprising:
orally administering 4-hydroxy-5-n-butoxy-5-oxopentanoic acid or 4-hydroxy-5-n-butoxy-5-oxopentanoic acid and tetracosanoic acid, where said tetracosanoic acid and 4-hydroxy-5-n-butoxy-5-oxopentanoic acid are obtained by
(a) extracting a plant material of Cakile arabica to obtain an extract;
(b) concentrating the extract to obtain a concentrate;
(c) chromatographing the concentrate into fractions; and
(d) isolating fractionated compounds.

9. The method according to claim 8 , wherein the plant material comprises the aerial parts of Cakile arabica.

10. The method according to claim 8 , wherein extracting is carried out by using at least one organic solvent.

11. The method according to claim 10 , wherein the organic solvent is selected from the group consisting of petroleum ether, heptane, hexane, ethanol, isopropanol, methanol and mixtures thereof, preferably is ethanol.

12. The method according to claim 8 , wherein chromatographing is carried out by means of thin layer chromatography (TLC) and/or column chromatography.

13. The method according to claim 12 , wherein the eluent used for chromatographing is petroleum ether, heptane, ethanol, methanol, benzene, diethyl ether, chloroform, dichloromethane, water and/or mixtures thereof.

14. The method according to claim 8 , wherein the stationary phase used for chromatographing is silica gel and/or aluminum oxide.

Assignees

• Univ King Saud

Inventors

• Shafeek Awaad Amani
• El-Meligy Reham Moustafa
• Marzouk Mohammed Sobhy
• Alotaiby Shroog Mohamed

Classifications

• A61K31/225

  Polycarboxylic acids · CPC title

• C07C51/47

  by solid-liquid treatment; by chemisorption · CPC title

• A61K36/31 Primary

  Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi · CPC title

• A61P1/04

  for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants · CPC title

• A61K2236/00

  Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine · CPC title