Automated selection of microorganisms and identification using MALDI

Patent metadata

Field	Value
Publication number	US-10859588-B2
Application number	US-201916668656-A
Country	US
Kind code	B2
Filing date	Oct 30, 2019
Priority date	Mar 30, 2012
Publication date	Dec 8, 2020
Grant date	Dec 8, 2020

Abstract

A method and apparatus for locating and selecting a colony of microorganisms on a culture dish and identifying microorganisms in said selected colony using MALDI. The method comprises the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of said selected colony of microorganisms; depositing at least some of said sample of said selected colony of microorganisms on a target plate; and transferring said target plate with said sample in an apparatus for performing MALDI for identification of said sample of said selected colony of microorganisms. A sample of a colony of microorganisms is automatically deposited on a depositing spot such that the sample covers at most approximately half of said one of the depositing spots of the target plate. A suspension of a sample of microorganisms is automatically prepared by automatically picking the sample with a picking tool and submerging the picking tool with said sample in a suspension, after which the picking tool is vibrated in vertical sense only to release the sample from the picking tool.

First claim

The invention claimed is:

1. A method comprising:
locating one or more colonies of microorganisms in a culture dish;
positioning a first picking tool in a picking tool holder of a positioning device;
obtaining, with the positioning device, a first sample of at least one of the located colonies of microorganisms, in part, by: (a) positioning the first picking tool above one of the located colonies of microorganisms in the culture dish, (b) automatically lowering the first picking tool towards the culture dish and into contact with the one of the located colonies of microorganisms, and (c) automatically raising the first picking tool with the sample of the one of the located colonies of microorganisms away from the culture dish;
depositing, with the positioning device, at least some of the first sample in a suspension tube containing a suspension medium, in part, by: (a) positioning the first picking tool above the suspension tube, (b) lowering the first picking tool such that at least some of the first sample is submerged in the suspension medium in the suspension tube, and (c) raising the first picking tool from the suspension tube;
measuring, with a turbidity meter, the turbidity of a suspension in the suspension tube comprising the at least some of the first sample and the suspension medium;
when the measured turbidity is above a first threshold value, dispensing an additional amount of suspension medium into the suspension tube; and
when the measured turbidity is below the first threshold value and above a second threshold value, providing a signal that the suspension tube holding the suspension can be further processed, wherein the first threshold value is equal to or greater than the second threshold value.

2. The method of claim 1 , wherein the turbidity meter comprises a sensor and a light source configured to transmit light through the suspension in the suspension tube for detection by the sensor.

3. The method of claim 1 further comprising:
when the measured turbidity is below the second threshold value:
obtaining, with the positioning device, a second sample of at least one of the located colonies of microorganisms, in part, by: (a) positioning the first picking tool or a second picking tool above one of the located colonies of microorganisms in the culture dish, wherein, if used, the second picking tool is previously positioned in the picking tool holder of the positioning device, (b) automatically lowering the first or second picking tool towards the culture dish and into contact with the one of the located colonies of microorganisms, and (c) automatically raising the first or second picking tool with the sample of the one of the located colonies of microorganisms away from the culture dish; and
depositing, with the positioning device, at least some of the second sample in the suspension in the suspension tube, in part, by: (a) positioning the first or second picking tool above the suspension tube, (b) lowering the first or second picking tool such that at least some of the second sample is submerged in the suspension medium in the suspension tube, and (c) raising the first or second picking tool from the suspension tube.

4. The method of claim 1 further comprising:
when a signal that the suspension tube holding the suspension can be further processed is provided, depositing, with the positioning device, at least some of the suspension in a test culture dish.

5. The method of claim 4 further comprising:
transferring the test culture dish with the at least some of the suspension to an apparatus for performing a susceptibility test.

6. The method of claim 1 further comprising:
when a signal that the suspension tube holding the suspension can be further processed is provided, depositing, with the positioning device, at least some of the suspension on at least one depositing spot of a target plate having a plurality of depositing spots.

7. The method of claim 6 further comprising:
transferring the target plate with the at least some of the first sample to an apparatus for performing MALDI for identification of the first sample.

8. The method of claim 6 further comprising
overlaying a drop of a MALDI matrix solution on the at least some of the suspension on the target plate.

9. The method of claim 6 , wherein the at least some of the suspension is deposited on at most approximately half of the at least one of the depositing spots of the target plate.

10. The method of claim 1 further comprising:
when a signal that the suspension tube holding the suspension can be further processed is provided, depositing, with the positioning device, at least some of the suspension in a test culture dish and on at least one depositing spot of a target plate having a plurality of depositing spots.

11. The method of claim 10 further comprising:
transferring the target plate with the at least some of the first sample to an apparatus for performing MALDI for identification of the first sample; and
transferring the test culture dish with the at least some of the suspension to an apparatus for performing a susceptibility test.

12. The method of claim 1 , wherein the step of locating the one or more colonies of microorganisms in the culture dish is performed, in part, by: (a) obtaining an initial image of the culture dish including all the colonies of microorganisms in the culture dish, (b) displaying the initial image on a display, and (c) manually selecting at least one colony of microorganisms in the initial image.

13. The method of claim 1 , wherein the step of locating the one or more colonies of microorganisms in the culture dish is performed, in part, by: (a) obtaining an initial image of the culture dish including all the colonies of microorganisms in the culture dish, (b) positioning the culture dish on a stage comprising an imaging device, (c) obtaining an image of the culture dish, and (d) automatically identifying at least one colony of microorganisms by comparing the image obtained by the imaging device with the initial image of the culture dish.

14. A system comprising:
a positioning device comprising a picking tool holder, wherein the picking tool holder is adapted to releasably engage a picking tool;
a turbidity meter;
an automatic suspension medium dispenser; and
one or more processors communicatively coupled to the positioning device, the turbidity meter, and the automatic suspension medium dispenser, wherein the one or more processors are arranged for automatically controlling (a) the movement of the positioning device, (b) the operation of the turbidity meter, and (c) the operation of the automatic suspension medium dispenser, and wherein the one or more processors are configured to:
obtain locations of one or more colonies of microorganisms in a culture dish;
control the positioning device to position a first picking tool in the picking tool holder;
control the positioning device to obtain a first sample of at least one of the located colonies of microorganisms in the culture dish, in part, by: (a) positioning the first picking tool above one of the located colonies of microorganisms in the culture dish, (b) automatically lowering the first picking tool towards the culture dish and into contact with the one of the located colonies of microorganisms, and (c) automatically raising the first picking tool with the sample of the one of the located colonies of microorganisms away from the culture dish;
control the positioning device to deposit at least some of the first sample in a suspension tube containing a suspension medium, in part, by: (a) positioning the first picking tool above the suspension tu

Assignees

• Bd Kiestra Bv

Inventors

• Botma Jetze
• Kleefstra Martijn
• Van Der Zee Tino Walter

Classifications

• G01N35/00

  Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor · CPC title

• G01N1/04

  in the solid state, e.g. by cutting · CPC title

• C12M1/26 Primary

  Inoculator or sampler · CPC title

• C12Q3/00

  Condition responsive control processes (apparatus therefor C12M1/36; controlling or regulating in general G05) · CPC title

• G01N35/10 Primary

  Devices for transferring samples {or any liquids} to, in, or from, the analysis apparatus, e.g. suction devices, injection devices {(G01N35/0099 takes precedence)} · CPC title