Methods and apparatus for handling microbial samples

Patent metadata

Field	Value
Publication number	US-9121005-B2
Application number	US-74074608-A
Country	US
Kind code	B2
Filing date	Nov 4, 2008
Priority date	Nov 5, 2007
Publication date	Sep 1, 2015
Grant date	Sep 1, 2015

Abstract

This invention pertains to the general field of microbiology, and more specifically to transfer, inoculation and/or streaking of micro-organisms, e.g. for the purpose of obtaining individual colonies. Provided is a method for streaking a microbial sample onto a solid carrier, comprising the steps of: a) contacting at least one ferromagnetic particle with a solid carrier, followed or preceded by providing the particle with at least part of said sample, and b) applying a magnetic field gradient to allow for magnetically controlled motion of said particle on said surface, such that at least part of the sample is streaked onto the solid carrier. Also provided is an apparatus for carrying out such method in an (semi-)automated fashion.

First claim

The invention claimed is:

1. A method for streaking a microbial sample onto a solid carrier, comprising:
providing a microbial sample;
providing at least one ferromagnetic particle optionally formed as a composite bead, wherein the particle or bead has a surface roughness (Ra) in the range of 0.1 to 25 μm, a diameter of between 2 to 8 mm and a density below 7 g/cm 3 ;
contacting a surface of said solid carrier with the particle;
providing the particle with at least part of said sample; and
applying a magnetic field gradient to allow for magnetically controlled motion of said particle on said surface in a predetermined pattern controlled by the magnetic field gradient, such that at least part of the sample is streaked onto the solid carrier wherein the solid carrier remains stationary while the sample is streaked onto the solid carrier by the magnetically controlled motion of the particle using the magnetic field gradient.

2. The method of claim 1 , wherein said carrier is selected from the group consisting of a glass slide, and a solid culture medium contained in culture plate.

3. The method of claim 1 , wherein said microbial sample is a clinical specimen.

4. The method of claim 3 , wherein the clinical specimen is selected from the group consisting of urine, an upper respiratory swab, a genital secretion, sputum, stool, pus, a sterile body fluid, a blood culture, and combinations of any thereof.

5. The method of claim 1 , wherein the particle has a surface roughness of between 2 and 15 μm.

6. The method of claim 5 , wherein the particle has a surface roughness of between 5 and 10 μm.

7. The method of claim 1 , wherein said particle comprises a hydrophilic surface.

8. The method of claim 1 , wherein said particle comprises at least one polymer and at least one magnetic material.

9. The method of claim 8 , wherein the particle comprises a magnetic core comprising a polymer coating.

10. The method according of claim 8 , wherein the particle comprises a polymer core comprising a magnetic coating.

11. The method of claim 8 , wherein the particle is made of a homogeneous mixture of at least one polymer and at least one magnetic material in particulate form.

12. The method of claim 11 , wherein the particle is made of a homogeneous mixture of at least one polymer and at least one magnetic powder.

13. The method of claim 8 , wherein the relative weight ratio of magnetic material to polymer is from between 10:90 and 30:70.

14. The method of claim 13 , wherein the relative weight ratio of magnetic material to polymer is from between 20:80 and 25:75.

15. The method of claim 1 , wherein the particle is comprised of a material selected from the group consisting of magnetite, ferrite, and combinations of any thereof.

16. The method of claim 1 , wherein the particle comprises a polymer that is heat resistant up to 120° C.

17. The method of claim 16 , wherein the particle comprises a polymer selected from the group consisting of an epoxy, a polyamide, a polypropylene, and combinations of any thereof.

18. The method of claim 1 , wherein the particle diameter is from 3-8 mm.

19. The method of claim 18 , wherein the particle diameter is from 4-6 mm.

20. The method of claim 1 , wherein the particle density is less than 5 g/cm 3 .

21. The method of claim 20 , wherein the particle density is less than 4 g/cm 3 .

22. A method for streaking a microbial sample onto a solid carrier, comprising:
providing a microbial sample;
providing at least one ferromagnetic particle optionally formed as a composite bead, wherein the particle or bead has a surface roughness (Ra) in the range of 0.1 to 25 μm, a diameter of between 2 to 8 mm and a density below 7 g/cm 3 ;
contacting a surface of said solid carrier with the particle;
providing the particle with at least part of said sample; and
applying a magnetic field gradient provided by a grid of multiple adjacent electromagnets to allow for magnetically controlled motion of said particle on said surface in a predetermined pattern controlled by the magnetic field gradient, such that at least part of the sample is streaked onto the solid carrier wherein the solid carrier remains stationary while the sample is streaked onto the solid carrier by the magnetically controlled motion of the particle using the magnetic field gradient provided by the grid.

Assignees

• Van Der Kaap Trienko Marten
• Kleefstra Martijn
• Tjernberg Ingeta Marie Sofie Edlund
• Botma Jetze
• Bd Kiestra Bv

Inventors

• Van Der Kaap Trienko Marten
• Kleefstra Martijn
• Tjernberg Ingeta Marie Sofie Edlund
• Botma Jetze

Classifications

• C12M33/02 Primary

  by impregnation, e.g. using swabs or loops (fluid transport using swabs B01L3/5029) · CPC title

• Y10T428/2982

  Particulate matter [e.g., sphere, flake, etc.] · CPC title

• C12Q1/24 Primary

  Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms · CPC title