Polypeptides having lysozyme activity and polynucleotides encoding same

Patent metadata

Field	Value
Publication number	US-10829751-B2
Application number	US-201816140941-A
Country	US
Kind code	B2
Filing date	Sep 25, 2018
Priority date	Nov 25, 2011
Publication date	Nov 10, 2020
Grant date	Nov 10, 2020

Abstract

The present invention relates to isolated polypeptides having lysozyme activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

First claim

The invention claimed is:

1. A method of feeding an animal, comprising administering a polypeptide having lysozyme activity to the animal, wherein the polypeptide has at least 90% sequence identity to the polypeptide of amino acids 1 to 208 of SEQ ID NO: 8.

2. The method of claim 1 , wherein the polypeptide has at least 92% sequence identity to the polypeptide sequence of amino acids 1 to 208 of SEQ ID NO: 8.

3. The method of claim 1 , wherein the polypeptide has at least 95% sequence identity to the polypeptide sequence of amino acids 1 to 208 of SEQ ID NO: 8.

4. The method of claim 1 , wherein the polypeptide has at least 97% sequence identity to the polypeptide sequence of amino acids 1 to 208 of SEQ ID NO: 8.

5. The method of claim 1 , wherein the polypeptide is encoded by a polynucleotide that hybridizes under high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO: 3 or SEQ ID NO: 7, or the full-length complement of thereof, and has at least 90% sequence identity to the polypeptide sequence of amino acids 1 to 208 of SEQ ID NO: 8.

6. The method of claim 1 , wherein the polypeptide is a variant of the polypeptide sequence of amino acids 1 to 208 of SEQ ID NO: 8, comprising a substitution, deletion, and/or insertion at one or more positions, and has at least 90% sequence identity to the polypeptide sequence of amino acids 1 to 208 of SEQ ID NO: 8.

7. The method of claim 1 , wherein the polypeptide is a fragment of the polypeptide sequence of amino acids 1 to 208 of SEQ ID NO: 8, which has lysozyme activity and has at least 90% sequence identity to the polypeptide sequence of amino acids 1 to 208 of SEQ ID NO: 8.

8. The method of claim 1 , wherein the polypeptide comprises amino acids 1 to 208 of SEQ ID NO: 8.

9. The method of claim 1 , wherein the polypeptide consists of amino acids 1 to 208 of SEQ ID NO: 8.

10. The method of claim 1 , wherein the animal is selected from the group consisting of sheep, goats, cattle, deer, pigs or swine, poultry; horses, and fish.

11. The method of claim 1 , wherein the animal is selected from the group consisting of beef cattle, cows, and young calves.

12. The method of claim 1 , wherein the animal is selected from the group consisting of geese, turkeys, ducks and chicken.

13. The method of claim 1 , wherein the animal is selected from the group consisting of broilers, chicks and layers.

14. The method of claim 1 , wherein the administering of the polypeptide having lysozyme activity improves animal performance.

15. The method of claim 1 , wherein the administering of the polypeptide having lysozyme activity suppresses growth or intestinal colonization of a viral pathogen, parasitic pathogen or bacterial pathogen.

16. The method of claim 15 , wherein the administering of the polypeptide having lysozyme activity suppresses growth or intestinal colonization of coccidian protozoa, Eimeria maxima , or Eimeria mitis.

17. The method of claim 15 , wherein the administering of the polypeptide having lysozyme activity suppresses growth or intestinal colonization of Clostridium perfringens, Escherichia coli, Campylobacter coli, C. hyointestinalis, C. jejuni, Yersinia ssp., Treponema suis, Brachyspira hyodysenteriae, Lawsonia intracellularis or Salmonella.

18. The method of claim 15 , wherein the animal is chicken and the administering of the polypeptide having lysozyme activity suppresses growth or intestinal colonization of Clostridium perfringens.

19. The method of claim 1 , wherein the polypeptide having lysozyme activity is administered to the animal in an animal feed or animal feed additive, which comprises at least one fat-soluble vitamin, and/or at least one water soluble vitamin, and/or at least one trace mineral, and/or at least one macro mineral.

20. The method of claim 19 , wherein the animal feed or animal feed additive further comprises at least one other enzyme selected from the group consisting of phytase (EC 3.1.3.8 or 3.1.3.26); xylanase (EC 3.2.1.8); galactanase (EC 3.2.1.89); alpha-galactosidase (EC 3.2.1.22); protease (EC 3.4), phospholipase A1 (EC 3.1.1.32); phospholipase A2 (EC 3.1.1.4); lysophospholipase (EC 3.1.1.5); phospholipase C (3.1.4.3); phospholipase D (EC 3.1.4.4); and amylase.

21. The method of claim 19 , wherein the animal feed or animal feed additive further comprises maize, sorghum, wheat, barley, oats, soybean meal, fish meal, meat and bone meal; and/or whey.

Assignees

• Novozymes As
• Novozyems As

Inventors

• Schnorr Kirk Matthew
• Nielsen Jens Erik
• Klausen Mikkel

Classifications

• Y02A40/818

  Alternative feeds for fish, e.g. in aquacultures · CPC title

• A23K20/20

  Inorganic substances, e.g. oligoelements · CPC title

• C11D3/38636

  containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase · CPC title

• C12N15/52 Primary

  Genes encoding for enzymes or proenzymes · CPC title

• C12N1/06

  Lysis of microorganisms · CPC title